An Amperometric Glucose Biosensor Based on Glucose Oxidase Immobilized in Electropolymerized Poly(o-aminophenol) and Carbon Nanotubes Composite Film on a Gold Electrode

Abstract

An amperometric glucose biosensor is developed that is based on immobilization of glucose oxidase (GOD) in a composite film of poly(o-aminophenol) (POAP) and carbon nanotubes (CNT), which are electrochemically copolymerized at a gold (Au) electrode. Because of the high surface per volume ratio and excellent electrical conductivity of CNT, the biosensor based on an Au/POAP/CNT/GOD electrode has lower detection limit (0.01 mM), larger maximum response current (0.24 mA cm -2 ) and higher sensitivity (11.4 mA M -1 cm -2 ) than the values of the biosensor based on an Au/POAP/GOD electrode. Additionally, the biosensor shows fast response time, large response current, and good antiinterferent ability for ascorbic acid, uric acid and acetaminophen. Good reproducibility and stability of the biosensor are also observed.

Poly-0-Aminophenol Obtained at High Potentials by Cyclic Voltammetry on Sn0(2): F Electrodes: Application in Quantitative Determination of Ascorbic Acid

“…One of such conditions is the substrate selected for the obtention of a conductive polymer. Among the commonest substrates utilized for this purpose are glassy carbon [1][2][3][4][5][6][7][8][9] , platinum 2,4,5,7,8,[10][11][12][13][14][15][16][17] , gold 4,7,[18][19][20][21][22][23][24][25][26][27] , carbon paste 28,29 , transparent indium tin oxide 18,19,30 , Cu 18 , Ag 31 , SnO 2 32 , etc. However, it is always advisable to employ other electrodic substrates for the fabrication of modified electrodes.…”

Electrosynthesis and Spectroscopic Characterization of Poly(o-Aminophenol) Film Electrodes

“…Thin nonconducting POAP films synthesized in neutral media are also employed as protective layers for biosensor applications. In general, POAP films are simultaneously synthesized with different electroactive materials such as, hemoglobin, quinhydrone, carbon nanotubes, prussian blue, and other polymers such as poly(o-phenylenediamine) and polypyrrole, which act as electron mediators [18,23,[28][29][30][31][32][33][34][35][36][37], to build different biosensors. POAP is also widely used as Figure 11: IR spectra of (a) electrochemically prepared POAP, (b) 3-aminophenoxazone [10].…”

Electrochemical sensors based on carbon nanotubes and their use in biomedical research.Part 2: sensors manufactured by dispersion of carbon nanotubes by means of polyethyleneimine, organic dyes, cyclodextrins, chitosan, proteins, room-temperature ionic liquids, gels, and thiols. Sensors manufactured by dispersion of carbon nanotubes by electropolymerization process. sensors manufactured by dispersion of carbon nanotubes by layer-by-layer deposition

“…В отличие от золотого электрода с плёнкой, не содержавшей углеродных нанотрубок, сенсор продемонстрировал высокие метрологические характеристики -предел обнаружения глюкозы соответствовал концентрации 1×10 -5 моль/л, а чувствительность определения равнялась 1,14×10 -2 А×л/(моль×см 2 ). Помимо этого, наблюдалось высокое быстродействие и стабильность функционирования сенсора, а также несущественное влияние на результаты измерений присутствовавших в пробе ацетаминофена, аскорбиновой и мочевой кислоты [36].…”

“…электрода. Помимо этого, нанесение нанотрубок на поверхность электрода может осуществляться и посредством электрополимеризации [32][33][34][35][36][37][38]. Вместе с тем, нанесение углеродных нанотрубок на рабочую поверхность электрода потенциально может сопровождаться формированием плёнки с низкой механической прочностью.…”

“…Получаемые сенсоры позволяют определять большое число химических соединений, являющихся характерными для биомедицинских исследований, в частности дофамин [2-4, 11, 15, 19, 26, 43], допак [2,4], адреналин [4,15,35], норадреналин [4,17,20], аскорбиновая [2,7,19] и мочевая [16,20] кислоты, пероксид водорода [2,4,5,22,25,[27][28][29][36][37][38], NADH [21,44], глюкоза [2,21,22,28,29,[36][37][38][39]44], фруктоза [44], холестерин [27], цитохром с [30], амитрол [4,5], диурон [5], монурон [5], хлортолурон [5], офлоксацин [12], эстрадиол [13], тимин …”