An Analysis of Volatile Components of the Liverworts Dumortiera hirsuta subsp. hirsuta and Dumortiera hirsuta subsp. nepalensis (Dumortieraceae) from Panama and Taxonomic Observations on the Species

Abstract

We report for the first time the chemical composition of volatile components (VOCs) of two subspecies, D. hirsuta subsp. hirsuta and D. hirsuta subsp. nepalensis, of the liverwort Dumortiera hirsuta from Panama by using headspace-solid phase microextraction-gas chromatography-mass spectrometry in order to assess distinguishing markers between the two species. Forty VOCs were identified in total for both subspecies. Of these, 34 are reported for the first time in D. hirsuta. Furthermore, both subspecies showed clear differences in the type and amount of VOCs. The major compounds in D. hirsuta subsp. hirsuta were α-gurjunene, β-selinene, α-guaiene, α-humulene and β-caryophyllene; while in D. hirsuta subsp. nepalensis were ledene, α-gurjunene, β-caryophyllene and α-guaiene, respectively. Two oxygenated sesquiterpenes, globulol and nerolidol, could be considered as possible distinguishing chemical markers between these two subspecies. We conclude that both morphotypes of D. hirsuta are chemically different.

Rapid Detection of Potential Natural Food Preservatives and Identification of Artemisia Species via High-Sensitivity Photoionization Mass Spectrometry

“…The PVOCs produced by Artemisia species are thought to be key to the ecological adaptation and evolution of Artemisia. 26 Moreover, PVOCs have the potential to be used as markers for accurately identifying certain plant subspecies that contain antibacterial substances 27 and to facilitate research on natural food preservatives. 28 Particularly, the development of phytogenic VTPs as natural food preservatives could be a promising area of research due to their potential antibacterial and antioxidant properties.…”

Chemical Profiling of Volatile Components of the Gametophyte and Sporophyte Stages of the Hornwort Leiosporoceros dussii (Leiosporocerotaceae) From Panama by HS-SPME-GC-MS

“…7 Solid phase microextraction (SPME) has been widely used for research and identification of volatile compounds present in plants, including bryophytes. 6,[8][9][10] SPME is a more sensitive and effective method, when compared with solvent extraction techniques for the analysis of VOCs in plants and fruits. 6,11 SPME is an analytical method that favors the isolation of volatile and semivolatile compounds present in small amounts in the sample, allowing for the identification of compounds otherwise undetectable by using other methods.…”

“…6,[8][9][10] SPME is a more sensitive and effective method, when compared with solvent extraction techniques for the analysis of VOCs in plants and fruits. 6,11 SPME is an analytical method that favors the isolation of volatile and semivolatile compounds present in small amounts in the sample, allowing for the identification of compounds otherwise undetectable by using other methods. In this paper, isolation and identification of chemical compounds from the gametophyte and sporophyte stages of L. dussii from Panama by headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) are reported for the first time.…”

“…The study of the volatile composition of bryophytes has facilitated the differentiation between species, and resolved important taxonomic questions. 5,6 Volatile organic compounds (VOCs) are remarkable secondary metabolites for protection of plants against environmental pathogens and predators. 7 Solid phase microextraction (SPME) has been widely used for research and identification of volatile compounds present in plants, including bryophytes.…”

Analysis of the Volatile Components of Pouteria sapota (Sapote Mamey) Fruit by HS-SPME-GC-MS

“…Headspace solid phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS), has been widely used for the study and identification of volatile organic compounds (VOCs) present in a wide array of plants [6][7][8][9] and fruits [10][11][12][13][14]. Solid phase microextraction (SPME) is a simple, effective, costsaving and solvent-free methodology for the analysis of VOCs and aromas in fruits [15].…”