An analytical method for prohexadione in Chinese cabbage and apple

Choi, Jeong‐Heui; Yoon, Hyung Joon; Do, Jung-Ah; Park, Yong-Chun; Kim, Jae‐Hun; Choi, Dukhyun

doi:10.1002/bmc.1472

Cited by 6 publications

(6 citation statements)

References 37 publications

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“…Daminozide, another 2OG analog and PGR similar to prohexadione, was sprayed on apple trees until 1989, before it was withdrawn due to concerns of its effect on human health. Prohexadione has been classified as a reduced risk pesticide by the Environmental Protection Agency due to its low toxicity and limited persistence in the environment due to photo and microbial degradations [9] , [27] . Although it meets the reduced risk criteria for pesticides, our results described in this article indicates that it is essential to set a stringent ‘Maximum Residue Limits’ for prohexadione to promote its safe use for food production.…”

Section: Discussionmentioning

confidence: 99%

Prohexadione, a plant growth regulator, inhibits histone lysine demethylases and modulates epigenetics

et al. 2014

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BackgroundEpigenetic modifications, particularly DNA methylation and posttranslational histone modifications regulate heritable changes in transcription without changes in the DNA sequence. Despite a number of studies showing clear links between environmental factors and DNA methylation, little is known about the effect of environmental factors on the recently identified histone lysine methylation. Since their identification numerous studies have establish critical role played by these enzymes in mammalian development.ObjectivesIdentification of the Jumonji (Jmj) domain containing histone lysine demethylase have added a new dimension to epigenetic control of gene expression by dynamic regulation of histone methylation marks. The objective of our study was to evaluate the effect of prohexadione and trinexapac, widely used plant growth regulators of the acylcyclohexanediones class, on the enzymatic activity of histone lysine demethylases and histone modifications during the neural stem/progenitor cell differentiation.MethodsHere we show that prohexadione, but not trinexapac, directly inhibits non-heme iron (II), 2-oxoglutarate-dependent histone lysine demethylase such as Jmjd2a. We used molecular modeling to show binding of prohexadione to Jmjd2a. We also performed in vitro demethylation assays to show the inhibitory effect of prohexadione on Jmjd2a. Further we tested this molecule in cell culture model of mouse hippocampal neural stem/progenitor cells to demonstrate its effect toward neuronal proliferation and differentiation.ResultsMolecular modeling studies suggest that prohexadione binds to the 2-oxoglutarate binding site of Jmjd2a demethylase. Treatment of primary neural stem/progenitor cells with prohexadione showed a concentration dependent reduction in their proliferation. Further, the prohexadione treated neurospheres were induced toward neurogenic lineage upon differentiation.ConclusionsOur results describe an important chemico-biological interaction of prohexadione, in light of critical roles played by histone lysine demethylases in human health and diseases.

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Section: Discussionmentioning

confidence: 99%

Prohexadione, a plant growth regulator, inhibits histone lysine demethylases and modulates epigenetics

et al. 2014

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“…It was found in four out of seven years with frequencies of detection ranging from 71% to 100% and mean concentrations 14.7-271 ng/L. Its use is approved in EU (Table S9) and is applied in fruits, vegetables and flowers [85][86][87][88][89]. The area covered by the WWTP of Athens is mainly urban and not much cultivation is presented.…”

Section: Plant Growth Regulatorsmentioning

confidence: 99%

Assessment of Environmental Pollution and Human Exposure to Pesticides by Wastewater Analysis in a Seven-Year Study in Athens, Greece

Rousis

Denardou

Alygizakis

et al. 2021

Toxics

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Pesticides have been used in large amounts around the world for decades and are responsible for environmental pollution and various adverse effects on human health. Analysis of untreated wastewater can deliver useful information on pesticides’ use in a particular area and allow the assessment of human exposure to certain substances. A wide-scope screening method, based on liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry, was applied, using both target and suspect screening methodologies. Daily composite influent wastewater samples were collected for seven or eight consecutive days in Athens between 2014 and 2020 and analyzed for 756 pesticides, their environmental transformation products and their human metabolites. Forty pesticides were quantified at mean concentrations up to 4.9 µg/L (tralkoxydim). The most abundant class was fungicides followed by herbicides, insect repellents, insecticides and plant growth regulators. In addition, pesticide transformation products and/or metabolites were detected with high frequency, indicating that research should be focused on them. Human exposure was evaluated using the wastewater-based epidemiology (WBE) approach and 3-ethyl-carbamoyl benzoic acid and cis-1,2,3,6-tetrahydrophthalimide were proposed as potential WBE biomarkers. Wastewater analysis revealed the presence of unapproved pesticides and indicated that there is an urgent need to include more transformation products in target databases.

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“…For example, daminozide, as a prohexadione-like analogue of 2-oxoglutarate and PGR, has been banned due to its effects on human health. The Environmental Protection Agency classifies prohexadione as a low-risk pesticide due to its low toxicity and limited resistance to the environment due to photo-and microbial degradation (Paulson et al, 2005;Choi et al, 2011). Although it meets the criteria of reduced environmental risk for pesticides, for prohexadione, in our opinion, it is necessary to establish clear "maximum residues" to ensure its safe use in food production.…”

Section: Features Of Action Of the Acylcyclohexadione Retardantsmentioning

confidence: 99%

Mode of physiological activity of acylcyclohexadione retardants

2020

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The key to high productivity of plants, especially cereals, is to control lodging of cereals. Among the derivatives of acylcyclohexadiones (ACHD), highly effective retardants have been invented, which are widely used in modern crop production to control the lodging of cereals, mainly winter wheat, spring and winter wheat, winter, spring and hybrids of barley. The mechanisms of influence of ACHD derivatives – trinexapac-ethyl and prohexadione-Ca on plants and features of their application in technologies of cultivation of cultivated plants are considered. By using a composition of ACHD derivatives with a number of agrochemicals, enhancement of retardant activity is achieved. Given the identified varietal variability of the effectiveness of the use of ACHD derivatives in cereals, it is advisable to conduct registration and post-registration studies primarily on varieties that occupy the largest sown areas in the country. The peculiarities of ACHD derivatives’ influence on stem components as the main one, along with the flag leaf, carbon pool for grain filling in the ear and possible reasons for the lack of productivity increase of grain crops due to ACHD derivatives are also considered. This class of substances continues to be widely studied in the world and remains among the most promising for the development of new agrochemicals and drugs.

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An analytical method for prohexadione in Chinese cabbage and apple

Cited by 6 publications

References 37 publications

Prohexadione, a plant growth regulator, inhibits histone lysine demethylases and modulates epigenetics

Prohexadione, a plant growth regulator, inhibits histone lysine demethylases and modulates epigenetics

Assessment of Environmental Pollution and Human Exposure to Pesticides by Wastewater Analysis in a Seven-Year Study in Athens, Greece

Mode of physiological activity of acylcyclohexadione retardants

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