An ancient C-type lectin in Chlamys farreri (CfLec-2) that mediate pathogen recognition and cellular adhesion

Yang, Jialong; Qiu, Limei; Wei, Xiumei; Wang, Leilei; Wang, Lingling; Zhou, Zhi; Zhang, Huan; Liu, Lin; Song, Linsheng

doi:10.1016/j.dci.2010.07.004

Cited by 64 publications

(49 citation statements)

References 57 publications

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“…A growing number of CTLs have recently been discovered from marine invertebrates, especially crustaceans and molluscs, including those from F. chinensis [16,21,46,47], L. vannamei [15,19,45,48], Penaeus monodon [49,50], C. farreri [18,23,24,26], A. irradians [22,25] and Crassostrea gigas [51], Crassostrea virginica [52], and Haliotis discus discus [17]. Relatively little information is available on CTLs in echinoderms, and a handful of sea cucumber CTLs have been reported, such as MBL-C (Cucumaria japonica) [53], SJL-I (S. japonicus) [35,36], MBL-AJ and AJCTL (A. japonicus) [37,38].…”

Section: Discussionmentioning

confidence: 99%

“…The PAMPs-binding ability of rAjCTL-1 was examined by enzyme-linked immunosorbent assay (ELISA) [24]. The 96-well microtiter plate was coated with lipopolysaccharide (LPS), peptidoglycan (PGN) and b-glucan (Glu) at 4 C overnight (20 mg/well, in 50 mM carbonate-bicarbonate buffer), blocked with 3% BSA in PBS at 37 C for 1 h, and then washed with PBST three times.…”

Section: Pamps Binding Assaymentioning

confidence: 99%

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Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

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a b s t r a c t C-type lectin is one important pattern recognition receptor (PRR) that plays crucial roles in multiple immune responses. A C-type lectin from sea cucumber Apostichopus japonicus (AjCTL-1) was characterized in the present study. The amino acid sequence of AjCTL-1 shared high similarities with other Ctype lectins from invertebrates and vertebrates. The C-type lectin domain (CTLD) of AjCTL-1 contained a Ca 2þ -binding site 2 and four conserved cysteine residues. AjCTL-1 mRNA expression patterns in tissues and after bacterial challenge were then analysed. Quantitative PCR revealed that AjCTL-1 mRNA was widely expressed in the tested tissues of healthy sea cucumber. The highest expression level occurred in gonad followed by body wall, coelomocytes, tentacle, intestinum and longitudinal muscle, and the lowest expression level was in respiratory tree. AjCTL-1 mRNA expression in coelomocytes was significantly induced by gram-negative Listonella anguillarum and gram-positive Micrococcus luteus, with different upregulation patterns post-challenge. Recombinant AjCTL-1 exhibited the ability to bind peptidoglycan directly, agglutinate M. luteus, Staphylococcus aureus and Escherichia coli, in a Ca 2þ -dependant manner, and enhance the phagocytosis of coelomocytes against E. coli in vitro. The results indicated that AjCTL-1 could act as a PRR in Apostichopus japonicus and had critical roles in non-self recognition and bacterial clearance against invading microbes.

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Section: Discussionmentioning

confidence: 99%

Section: Pamps Binding Assaymentioning

confidence: 99%

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

Self Cite

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“…Similar result was also observed in present study, SgCTL-1 could be induced by stimulation of LPS and glucan, indicating SgCTL-1 was involved in the recognition toward gramnegative bacteria and fungi. Interestingly, the expression of SgCTL-1 could be more significantly up-regulated by PGN, which was different with other mollusk C-type lectins [5,54], suggesting the main function of SgCTL-1 might be recognizing gram-positive bacteria and triggering relative immune responses. Although the mRNA expression of other galectins, such as galectins from scallop and amphioxus was significantly up-regulated after challenge of both gram-positive and gram-negative bacteria, they were almost stable during the fungi stimulation [40,55].…”

Section: Discussionmentioning

confidence: 96%

“…As two important PRRs, C-type lectin and galectin could recognize mannose/galactose and galactoside, respectively, and they played different roles in innate immunity [5,6]. To investigate the potential function SgCTL-1 and SgGal-1 performed in immune recognition, the expression pattern of the two lectin genes were determined after razor clams were stimulated by three PAMPs from microorganisms.…”

Section: Discussionmentioning

confidence: 99%

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Identification and transcriptional analysis of two types of lectins (SgCTL-1 and SgGal-1) from mollusk Solen grandis

Wei

Yang

Liu

et al. 2012

Fish & Shellfish Immunology

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Identification and characterization of two novel C‐type lectins from the larvae of housefly, Musca domestica L.

Zhou

Fang

Zheng

et al. 2018

Arch Insect Biochem Physiol

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Lectins and antimicrobial peptides (AMPs) are widely distributed in various insects and play crucial roles in primary host defense against pathogenic microorganisms. Two AMPs (cecropin and attacin) have been identified and characterized in the larvae of housefly. In this study, two novel C-type lectins (CTLs) were obtained from Musca domestica, while their agglutinating and antiviral properties were evaluated. Real-time PCR analysis showed that the mRNA levels of four immune genes (MdCTL1, MdCTL2, Cecropin, and Attacin) from M. domestica were significantly upregulated after injection with killed Gram-negative Escherichia coli. Moreover, purified MdCTL1-2 proteins can agglutinate E. coli and Staphylococcus aureus in the presence of calcium ions, suggesting their immune function is Ca dependent. Sequence analysis indicated that typical WND and QPD motifs were found in the Ca -binding site 2 of carbohydrate recognition domain from MdCTL1-2, which was consistent with their agglutinating activities. Subsequently, antiviral experiments indicated that MdCTL1-2 proteins could significantly reduce the infection rate of Spodoptera frugiperda 9 cells by the baculovirus Autographa californica multicapsid nucleopolyhedrovirus, indicating they might play important roles in insect innate immunity against microbial pathogens. In addition, MdCTL1-2 proteins could effectively inhibit the replication of influenza H N virus, which was similar to the effect of ribavirin. These results suggested that two novel CTLs could be considered a promising drug candidate for the treatment of influenza. Moreover, it is believed that the discovery of the CTLs with antiviral effects in M. domestica will improve our understanding of the molecular mechanism of insect immune response against viruses.

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An ancient C-type lectin in Chlamys farreri (CfLec-2) that mediate pathogen recognition and cellular adhesion

Cited by 64 publications

References 57 publications

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Identification and transcriptional analysis of two types of lectins (SgCTL-1 and SgGal-1) from mollusk Solen grandis

Identification and characterization of two novel C‐type lectins from the larvae of housefly, Musca domestica L.

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