An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae

Han, Chao; Wang, Tao; Jiang, Hongqiang; Ma, Jianxiong; Tian, Peng; Zang, Jiacheng; Ma, Xinlong

doi:10.1038/srep35102

Cited by 18 publications

(8 citation statements)

References 47 publications

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“…In our animal model, no Modic changes were observed in the degenerated or regenerated IVDs, perhaps because Modic changes are usually associated with chronic degeneration of the IVD. 54 A long-term study would be needed to explore the effects of cell therapy on Modic changes.…”

Section: Discussionmentioning

confidence: 99%

Potential of Human Nucleus Pulposus-Like Cells Derived From Umbilical Cord to Treat Degenerative Disc Disease

et al. 2018

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Section: Discussionmentioning

confidence: 99%

Potential of Human Nucleus Pulposus-Like Cells Derived From Umbilical Cord to Treat Degenerative Disc Disease

et al. 2018

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“…Total protein was extracted from the cells and tissues in RIPA lysis buffer as described previously [22]. Briefly, forty micrograms of protein were loaded per lane and separated using 10% SDS-PAGE gel electrophoresis and then transferred to PVDF membranes.…”

Section: Methodsmentioning

confidence: 99%

Effect of Liuweibuqi Capsules in Pulmonary Alveolar Epithelial Cells and COPD Through JAK/STAT Pathway

Wang¹,

Ding²,

Tang³

et al. 2017

Cell Physiol Biochem

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Background/Aims: To evaluate the effect of Liuweibuqi capsules on chronic obstructive pulmonary disease (COPD) through the JAK/STAT pathway. Methods: Lung function was measured with a spirometer. Changes in lung histology were observed using H&E staining. Cigarette smoke extract combined with lipopolysaccharide (CSE+LPS) was used to establish the cellular COPD model. Cytokine levels were determined using ELISA, and changes in the JAK/STAT pathway were evaluated using western blotting. The CCK8 method and flow cytometry were used to measure cell viability and apoptosis, respectively. Results: Liuweibuqi capsules reduced the damage in the lung tissues and the loss of lung function in the COPD rats. Additionally, the levels of interleukin (IL)-1β, interferon γ (IFNγ), IL-6, tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β1 were higher, whereas IL-10 was lower in the model control (MC) and CSE+LPS groups than in the normal group. The phosphorylation levels of JAK1, JAK2, STAT1, STAT3 and STAT5 were higher and the levels of SOCS1 and SOCS3 were lower in the MC group and CSE+LPS group compared with the normal group. After Liuweibuqi capsule treatment, the expression of inflammatory cytokines and elements of the JAK/STAT pathway were lower. In addition, over-expression of STAT3 blocked the effects of the Liuweibuqi capsules on the release of inflammatory cytokines, cell viability and apoptosis. Conclusion: Our findings suggested that Liuweibuqi capsule might effectively ameliorate the progression of COPD via the JAK/STAT pathway.

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“…They are considered as probably a disc degeneration-related process, and there are a few concepts about the background of these changes [ 32 – 34 ]. Han et al described that high expression of IFN- γ was detected in the group with the embedment of nucleus pulposus into subchondral bone in an animal model [ 35 ]. In our study, the serum level of IFN- γ in patients with positive Modic changes is significantly smaller than that in patients without confirmed Modic changes.…”

Section: Discussionmentioning

confidence: 99%

IFN-γ Correlations with Pain Assessment, Radiological Findings, and Clinical Intercourse in Patient after Lumbar Microdiscectomy: Preliminary Study

et al. 2020

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Objectives. We investigated the influence of pain decrease after lumbar microdiscectomy on the interferon gamma (IFN-γ) serum level in patients with lumbar disc herniations. The study challenges the mechanism of sciatica pain and the role of IFN-γ in radicular pain development. Material and Methods. We performed clinical and immunoenzymatic assessment in a group of 27 patients with lumbar radicular pain due to disc herniations before and 3 months after surgery. Clinical status was assessed with the use of the Numeric Rating Scale (NRS), the Pain Rating Index and Pain Intensity Index of McGill Pain Questionnaire (SF-MPQ), the Oswestry Disability Index (ODI), and Beck Depression Inventory (BDI). The plasma concentrations of IFN-γ were ascertained by an immunoenzymatic method. Results. We observe significant correlations between the results of the pain in the back region assessment NRS back scale after the surgery with the level of IFN-γ before the procedure ( r s = 0.528 ; p = 0.008 ) and after the procedure ( r s = 0.455 ; p = 0.025 ). These are moderate and positive correlations—the decrease in pain is correlated with the lower IFN-γ level. Additionally, there are significant correlations between the results of the PRI scale and the IFN-γ level. The PRI score before surgery correlates positively with IFN-γ after surgery ( r s = 0.462 ; p = 0.023 ), and the PRI score after surgery correlates positively with IFN before surgery ( r s = 0.529 ; p = 0.005 ) and after surgery ( r s = 0.549 ; p = 0.003 ). All correlations are moderate in severity—severe pain before surgery correlates with a higher level of IFN-γ after surgery and also higher IFN-γ before surgery. There were significant differences in the IFN-γ level before ( Z = − 2.733 ; p = 0.006 ) and after ( Z = − 2.391 ; p = 0.017 ) surgery in the groups of patients with and without nerve compression. In the group of patients with nerve compression, the level of IFN-γ before and after surgery was lower. Conclusions. Less pain ratio after operation correlates with the level of IFN-γ. In the group of patients without significant nerve compression confirmed by MRI scans, the level of IFN-γ before and after surgery was higher than that in the group with nerve root compression.

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An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae

Cited by 18 publications

References 47 publications

Potential of Human Nucleus Pulposus-Like Cells Derived From Umbilical Cord to Treat Degenerative Disc Disease

Potential of Human Nucleus Pulposus-Like Cells Derived From Umbilical Cord to Treat Degenerative Disc Disease

Effect of Liuweibuqi Capsules in Pulmonary Alveolar Epithelial Cells and COPD Through JAK/STAT Pathway

IFN-γ Correlations with Pain Assessment, Radiological Findings, and Clinical Intercourse in Patient after Lumbar Microdiscectomy: Preliminary Study

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