An antibiotic-free medium for the xenic cultivation of Entamoeba gingivalis

Gannon, John T.; Linke, H. A. B.

doi:10.1016/0020-7519(91)90097-q

Cited by 4 publications

(2 citation statements)

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“…Construction of the Primers to Amplify Protozoan SrRNA-Genes Since the standard strains of E. gingivalis ATCC 30927 and 30928 can be obtained only as xenic cultures which contain several species of bacteria (8,9,(12)(13)(14)(15), the SrRNA gene of E. gingivalis was supposed to be amplified by PCR only with protozoan (or at least eukaryote)-specific primers. Hence, the SrRNA sequences of 16 eukaryotic genera and 7 prokaryotic genera (25) were obtained from GenBank and aligned manually with that of E. histolytica reported by Que and Reed (27).…”

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confidence: 99%

“…E. gingivalis is normally found in the human oral cavity (6,7,19,20,24), and sometimes elsewhere, for example in sputum (6) or in genital tracts of women using intrauterine devices (5,8). Although some authors suggested that E. gingivalis might be involved in periodontal diseases (7,19,20), the ecology and the pathogenicity of the protozoan are still uncertain; mainly because it can not be cultivated as a pure culture (9,(12)(13)(14)(15).…”

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Nucleotide Sequence of the SrRNA Gene of Entamoeba gingivalis: Applications for Construction of a Species‐Specific DNA Probe and Phylogenetic Analysis

Yamamoto

Kikuta

Hashimoto

et al. 1995

Microbiology and Immunology

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The small subunit ribosomal RNA (SrRNA) gene of Entamoeba gingivalis was amplified by PCR and the product of 1.9-kbp sequence was cloned into a plasmid vector pUC18. Four clones were isolated and sequenced. The insert DNAs were 1918-to 1921-bp long and A+ T rich (65.5 %). The four SrRNA sequences of E. gingivalis were found to be aligned with those of nine related protozoans while searching for E. gingivalis-specific sequences. A sequence of 28 oligonucleotides was chosen, chemically synthesized, and labeled with digoxigenin for use as a DNA probe. The probe thus constructed was shown to hybridize only with either the SrRNA-coding DNAs or the cells of the two E. gingivalis strains and not with those of other protozoans or oral fungi tested. A representative SrRNA-sequence was analyzed and a phylogenetic tree was constructed by the neighbor-joining (NJ) method. Among the protists examined, E. gingivalis was placed next to Entamoeba histolytica as expected from the traditional taxonomy.

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