An antimicrobial peptide Ar-AMP from amaranth (Amaranthus retroflexus L.) seeds

Липкин, А.В.; Anisimova, V. E.; Nikonorova, A. K.; Бабаков, А. В.; Krause, Eberhardt; Bienert, Mikhael; Grishin, Eugene V.; Egorov, Tsezi A.

doi:10.1016/j.phytochem.2005.07.015

Cited by 83 publications

(73 citation statements)

References 14 publications

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“…The trend for antifungal activity was 100% methanol leaf > 100 methanol seed > 80% methanol leaf > 80% methanol seed. The currents results support the earlier findings which demonstrate the presence of antimicrobial activity in seeds of Amarathaceae [30,31]. …”

Section: Antimicrobial Activitysupporting

confidence: 92%

Phytochemical Profiling with Antioxidant and Antimicrobial Screening of <i>Amaranthus viridis</i> L. Leaf and Seed Extracts

Ahmed¹,

Hanif²,

Iftkhar³

2013

OJMM

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Methanol extracts of the dried leaves and seeds of Amaranthus viridis were collected and used for phytochemicals and antibacterial analysis. By detecting the MIC and zone inhibition, the antibacterial activity was determined against different bacterial and fungal strains. The extract yields from the leaves and seeds ranged 5.5 -6.1 and 2.42% -3.72% w/w, respectively. Phytochemical investigation of this plant determines that tanins (6.07% -5.96%), saponins (53% -32%), alkaloids (13.14% -11.42%), protiens (16.76% -24.51%) and glycosides (63.2% -32.3%) were rich in leaves. The extracts also contained appreciable levels of total phenolic contents (2.81 -3.61 GAE, g/100 g), total flavanoid contents (18.4 -5.42 QE, g/100 g) and DPPH free radical scavenging activity, showing IC 50 (83.45 -75.95 µg/mL) along with reducing power was calculated. The MIC of extracts ranged 178 -645 µg/mL. The results of this study suggest the possibility of using the methanolic extracts in treating the diseases caused by the test organisms.

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Section: Antimicrobial Activitysupporting

confidence: 92%

Phytochemical Profiling with Antioxidant and Antimicrobial Screening of <i>Amaranthus viridis</i> L. Leaf and Seed Extracts

Ahmed¹,

Hanif²,

Iftkhar³

2013

OJMM

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“…To obtain acid extracts, the powdered material (2 g) of all plants was extracted (ratio 1:10) with 10% (v/v) acetic acid for 1 h on a magnetic stirrer at room temperature (Lipkin et al, 2005). The homogenate was filtered through double-layered muslin cloth and clarified by centrifugation at 7000 x g for 30 min at 4 °C.…”

Section: Preparation Of Plant Extractsmentioning

confidence: 99%

Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp

Dellavalle

Cabrera

Alem

et al. 2011

Chilean J. Agric. Res.

154

101

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The aim of the study was to evaluate the antifungal activity of extracts of 10 plant species used in traditional Uruguayan medicine against the phytopathogenic fungus Alternaria spp. The plants were selected on the basis of their reported ethnobotanical uses. Aqueous, saline buffer and acid extracts of different plant species were screened in vitro for their antifungal activity against Alternaria spp. For the antifungal evaluation we used a microspectrophotometric assay. Minimal inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the extracts were determined. Three solvents were assayed on different tissues of the plants and among the 29 evaluated extracts, 31% of the extracts inhibited growth, similar to the effects of a chemical fungicide. Acid extracts of the plants were more effective than the aqueous or buffer extracts against Alternaria spp. The MIC values of the extracts were determined ranging between 1.25 and 25 µg mL -1 . The MFC values of the extracts ranged between 1.25 µg mL -1(Rosmarinus officinalis L.) and 10 µg mL -1 (Cynara scolymus L.). MICs and MFCs values obtained from leaves (Salvia officinalis L. and R. officinalis) and seeds extracts (Salvia sclarea L.) were quite comparable to values obtained with the conventional fungicide captan (2.5 µg mL -1 ). The extracts of Salvia sclarea, S. officinalis and R. officinalis could be considered as potential sources of antifungal compounds for treating diseases in plants. These extracts showed maximum activity, even at very low concentrations, and the same fungicide effects as chemical fungicide. We conclude from this that these extracts exhibit amazing fungicidal properties that support their traditional use as antiseptics.

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“…Hevein-like peptides with antifungical activities at micromolar or lower concentrations have been purified from Eucommia ulmoides bark, ginkgo leafs, elderberry fruits and seeds of amaranth, morning glory, or wheat (Koo et al 1998;Van Damme et al 1999;Huang et al 2000;Huang et al 2002;Lipkin et al 2005;Odintsova et al 2009). These peptides appear to be expressed constitutively, suggesting a possible preventive role against fungal attacks.…”

Section: Hevein-like Peptidesmentioning

confidence: 99%

New Antimicrobial Agents of Plant Origin

Sampedro

Valdivia

2013

Antimicrobial Compounds

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Plants are constantly under attack by microbial pathogens. As part of their defensive arsenal, they use antimicrobial peptides such as thionins, defensins, lipid transfer proteins, hevein-like peptides, knottins, cyclotides, b-barrelins, and others. In addition, they produce a diversity of antimicrobial metabolites. Those where the evidence for a role in plant defense is stronger include benzoxazinoids, camalexin, and glucosinolates among the alkaloids; flavonoids and stilbenes among the phenylpropanoids; and also terpenoids such as saponins. Our understanding of these plant antimicrobial agents has increased significantly in recent years with new information on their distribution, synthesis, regulation, in vivo function, and mechanism of action. Plant antimicrobial agents have a large potential for biotechnological applications. Engineered plants with increased disease resistance have been achieved using almost every family of antimicrobial peptides. There have been also been successes in using metabolic engineering to increase the production of antimicrobial compounds, as in the case of stilbenes and glucosinolates. Commercial applications using both approaches are likely to appear soon. The use of plant antimicrobials in human medicine is probably further in the future, although there are promising antifungal agents like defensin peptides, and saponins. With more than a quarter million species and a particularly diverse specialized metabolism, the richness of plant antimicrobials has barely been explored. Plant Antimicrobial DefensesWild plants are quite successful at keeping bacterial and fungal pathogens at bay. In addition to physical barriers, they have an immune system capable of detecting and responding to the presence of pathogens. The first layer of defense is based on

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An antimicrobial peptide Ar-AMP from amaranth (Amaranthus retroflexus L.) seeds

Cited by 83 publications

References 14 publications

Phytochemical Profiling with Antioxidant and Antimicrobial Screening of <i>Amaranthus viridis</i> L. Leaf and Seed Extracts

Phytochemical Profiling with Antioxidant and Antimicrobial Screening of <i>Amaranthus viridis</i> L. Leaf and Seed Extracts

Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp

New Antimicrobial Agents of Plant Origin

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An antimicrobial peptide Ar-AMP from amaranth (Amaranthus retroflexus L.) seeds

Cited by 83 publications

References 14 publications

Phytochemical Profiling with Antioxidant and Antimicrobial Screening of &lt;i&gt;Amaranthus viridis&lt;/i&gt; L. Leaf and Seed Extracts

Phytochemical Profiling with Antioxidant and Antimicrobial Screening of &lt;i&gt;Amaranthus viridis&lt;/i&gt; L. Leaf and Seed Extracts

Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp

New Antimicrobial Agents of Plant Origin

Contact Info

Product

Resources

About

Phytochemical Profiling with Antioxidant and Antimicrobial Screening of <i>Amaranthus viridis</i> L. Leaf and Seed Extracts

Phytochemical Profiling with Antioxidant and Antimicrobial Screening of <i>Amaranthus viridis</i> L. Leaf and Seed Extracts