An approach based on consecutive high-speed counter-current chromatography for preparation of an active compound rutin from <i>Apocynum venetum</i> L

Meng

et al. 2022

Self Cite

Morus alba leaves are a natural product with great antidiabetic potential. However, the therapeutic efficacy of natural products is usually achieved through the interaction of active compounds with specific targets. Among them, active compounds with multi‐target therapeutic functions are more effective than single‐target enzymes. In this study, a bienzyme system was constructed by co‐immobilizing α‐amylase and α‐glucosidase onto Fe3O4 for affinity screening of dual‐target active components in the complex extract from M. alba leaves. As a result, a potential active compound was selectively screened by ligand fishing, separated by high‐speed countercurrent chromatography using a solvent system of ethyl acetate–n‐butanol–water (3:2:5, v/v), and identified as rutin. In addition, the result of molecular docking showed that rutin could interact with the active center of α‐amylase and α‐glucosidase through multiple hydrogen bonds, van der Waals forces, etc. to play an inhibitory role. These results demonstrate the effectiveness of the polydopamine magnetically immobilized bienzyme system for dual‐target affinity screening of active substances. This study not only reveals the chemical basis of the antidiabetic activity of M. alba leaves from a dual‐target perspective, but also promotes the progress of multitarget affinity screening.

Section: Resultsmentioning

confidence: 89%

“…Since rutin is an important natural compound with a wide range of biological activities (Li et al, 2021), it is not difficult to find evidence for rutin inhibiting α ‐amylase and α ‐glucosidase in previous studies. Most of the previous studies used acarbose as a positive control to investigate the effect of rutin on enzyme activity.…”

Section: Resultsmentioning

confidence: 99%

Dual‐target affinity analysis and separation of α‐amylase and α‐glucosidase inhibitors from Morus alba leaves using a magnetic bifunctional immobilized enzyme system

Guo

Meng

et al. 2022

Self Cite

“…The effluent was monitored at 254 nm. The conditions of consecutive separation were consistent with those of traditional HSCCC (Kang et al, 2016;Li et al, 2021;Yang, Wang, et al, 2020). The interval loading time of the crude sample was calculated using Equation (1):…”

Section: Separation Processmentioning

confidence: 99%

Ellagic acid from pomegranate peel: Consecutive countercurrent chromatographic separation and antioxidant effect

Yang

et al. 2023

Self Cite

Ellagic acid is one of the most representative natural antioxidants, and is rich in pomegranate peel. In this study, a consecutive countercurrent chromatographic (CCC) separation method was established to improve the preparative efficiency of ellagic acid from pomegranate peel. By optimizing the solvent system, sample size and flow rate, 280 mg of ellagic acid was obtained from 5 g of crude sample from pomegranate peel by CCC after six consecutive injections. Moreover, the values of EC50 for ellagic acid in scavenging ABTS·+ and DPPH· were 4.59 ± 0.07 and 10.54 ± 0.07 μg/ml, respectively, indicating a strong antioxidant activity. This study not only established a high‐throughput method for the preparation of ellagic acid, but also provided a successful example for the development of and research on other natural antioxidants.

“…The target compound can be repeatedly separated and enriched using a commercial counter-current chromatography (CCC) column with a volume of 300 ml (Li et al, 2021;Lv et al, 2015;Yang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, consecutive HSCCC separation is a good choice to prepare samples on a large scale (Bouju et al, 2015; Köhler & Winterhalter, 2005), because HSCCC separation has good stability. The target compound can be repeatedly separated and enriched using a commercial counter‐current chromatography (CCC) column with a volume of 300 ml (Li et al, 2021; Lv et al, 2015; Yang et al, 2020). Although 5‐HMF has been successfully separated by traditional HSCCC, its separation is still used as a case study to develop a consecutive HSCCC separation method, which can not only increase the yield of 5‐HMF, but also save time and solvent.…”

Section: Introductionmentioning

confidence: 99%

Preparation of 5‐hydroxymethylfurfural from Schisandra chinensis (Turcz.) Baill by high‐speed counter‐current chromatography: Comparison of conventional and consecutive separation

Tang

et al. 2022

Self Cite

Schisandra chinensis is a plant with high medicinal value, which contains many medicinal ingredients, including 5-hydroxymethylfurfural. In the present study, an efficient method based on high-speed counter-current chromatography was established for the preparation of 5-hydroxymethylfurfural from Schisandra chinensis. Petroleum ether-ethyl acetate-methanol-water (2:5:2:5, v/v) was selected as the solvent system for high-speed counter-current chromatography. In order to improve the yield of single separation, the sample size was continuously optimized and improved. The results showed that 1,250 mg was the most suitable sample size, and 41 mg of the target compound with 97% purity was obtained in a single run. To further improve the yield, consecutive high-speed counter-current chromatography was introduced and compared with the results of a high-speed counter-current chromatography single run. The results showed that although the purity was reduced to 92%, 430 mg of the target compound was obtained from 12.5 g of ethanol extract within 670 min after 10 consecutive injections. This indicated that consecutive separation not only increased the yield of the target compound, but also saved the separation time and greatly improved the separation efficiency of high-speed counter-current chromatography.