An approach to a cold chain free oral cholera vaccine: in vitro and in vivo characterization of Vibrio cholerae gastro-resistant microparticles

Delivery strategies to enhance oral vaccination against enteric infections

“…Strategies to increase the temperature stability of vaccine components are required in parallel with innovative delivery strategies. In this context, the process of vitrification [22] to enhance stability is an attractive strategy, possibly combined with approaches such as gastric resistant microcapsules [23]. As with other oral delivery strategies it will be essential that the priorities of thermostabilisation and gastrointestinal delivery challenges are addressed in an integrated manner.…”

Current and New Approaches for Mucosal Vaccine Delivery

“…A single oral dose of alginateencapsulated BCG elicited effective long-lasting mucosal and systemic immune responses [147]. Cold-chain-free OCV could be developed by encapsulating heat-inactivated bacterial cells with alginate [148]. Oral vaccines against Edwardsiella, Brucella, and Aeromonas infections were also developed by encapsulation with alginate [149À152].…”

Particulate delivery systems for vaccination against bioterrorism agents and emerging infectious pathogens

“…Upon oral immunization in mice, the PLA/PEG formulation elicited the highest antigen-specific IgG, IgA, and IgM titers and a superior survival rate post-challenge. In an alternative approach, an oral cholera vaccine was produced by encapsulating inactivated V. cholerae into microparticles composed of the enteric excipient Eudragit ® and mucoadhesive agents alginate or Carbopol ® 120, 121 . Following oral vaccination, the formulation incorporating alginate elicited higher vibriocidal titers than a soluble vaccine, demonstrating the promise of mucoadhesive particles for oral vaccination against cholera.…”