An approach toward miRNA detection<i>via</i>different thermo-responsive aggregation/disaggregation of CdTe quantum dots

Borghei, Yasaman Sadat; Hosseini, Morteza

doi:10.1039/c8ra04257h

Cited by 17 publications

(11 citation statements)

References 45 publications

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“…As the density of conjugated DNAs may greatly affect the sensitivity in many hybridization‐based biosensing applications such as microRNA detection (20), hybridization tests were therefore conducted to verify the functionality of poly G‐mediated pDNA‐AuNP. If the resulting structure from the synthesis of AuNP on extra strand poly G 12 is mDNA‐AuNPs conjugates, then hybridization causes the formation of composed dimers (Scheme 1a), while the spherical‐shaped aggregates were observed for the pDNA‐AuNPs conjugates with linker DNA sequences (Scheme 1b).…”

Section: Resultsmentioning

confidence: 99%

Building Polyvalent DNA‐Functionalized Anisotropic AuNPs using Poly‐Guanine‐Mediated In‐Situ Synthesis for LSPR‐Based Assays: Case Study on OncomiR‐155

Borghei

Hosseinkhani

2022

Photochem & Photobiology

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DNA-functionalized gold nanoparticles (DNA-AuNPs) hold great promise for numerous biomedical applications, especially the building of well-defined nanosystems. Previously reported methods for the preparation of DNA-AuNPs all rely on the use of DNA-bearing free thiol or disulfide groups at their 3 0 /5 0 ends. But here we report a novel polyvalent DNA-AuNPs conjugation approach by in-situ fast synthesis of AuNPs at the polyguanine (G 12 ) strands. As confirmed by both TEM images and gel electrophoresis analysis, many poly G strand can form an individual anisotropic AuNP and so each AuNP functionalized with a dense layer of DNA, resulting in the formation of polyvalent (p)DNA-AuNPs. The general applicability of this novel approach was further verified in hybridization test and UV-Vis spectroscopy results show that pDNA-AuNPs conjugation is more attractive in biomedical diagnosis and specific sequence detection like microRNA-155 by using an extra-strand poly G with "sticky end" that are complementary to the target sequence.

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Section: Resultsmentioning

confidence: 99%

Building Polyvalent DNA‐Functionalized Anisotropic AuNPs using Poly‐Guanine‐Mediated In‐Situ Synthesis for LSPR‐Based Assays: Case Study on OncomiR‐155

Borghei

Hosseinkhani

2022

Photochem & Photobiology

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“…Then, the coated paper was placed, in a solution containing the patient’s throat swab sample, similar to a reported approach. 18 …”

Section: Methodsmentioning

confidence: 99%

“…To perform the sensing test, first, 5 μL of four ASO@AuNPs were immobilized on the detection zone by simple physical adsorption on paper, carried out by pouring a mixture (20 μL) of four ASO@AuNPs on the bare Whatman No.1 paper platform. Then, the coated paper was placed, in a solution containing the patient’s throat swab sample, similar to a reported approach …”

Section: Methodsmentioning

confidence: 99%

Exploitation of N-Gene of SARS-CoV-2 to Develop a New Rapid Assay by ASOs@AuNPs

2022

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A naked-eye (equipment-free), label-free (cost-effective), and RNA extraction-free (to speed up) method for SARS-CoV-2 (as a case study of RNA viruses) detection is developed. Here, the DNA is being used as a template for in situ formation of anisotropic gold nanoparticles (AuNPs) without any chemical modification or DNA labeling. In this study, synthesized AuNPs for the direct detection of N-gene (nucleocapsid phosphoprotein) of SARS-CoV-2 are exploited. To this aim, antisense oligonucleotides (ASOs) with an extra poly guanine tail (G12) were designed. Thus, in the presence of its viral target RNA gene and ASOs@AuNPs-RNA hybridization, there was a red shift in its localized surface plasmon resonance (LSPR), and the intensity of the LSPR peak at 690 nm of throat swab samples was compared to the threshold cycle (Ct) of a reverse-transcriptase real-time polymerase chain reaction (RT-qPCR) (as a gold standard). Results suggested that the plasmonic biosensor can detect a very low amount of SARS-CoV-2 with a detection limit close to RT-qPCR. Simplicity of the new conjugation method with hybridization and annealing without amplification and denaturation steps enabled it to perform in a microfluidic paper-based analytical device.

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“…They are also characterized by their high quantum function, large molar extinction coefficients, narrow/wide absorption, balanced photoluminescence (PL), near infrared (NIR) UV spectrum and high efficiencies compared with other fluorescence probes [17]. These features have made them interesting for their possible application in industry such as in light emitting diodes (LEDs), solar cells, lasers, absorption filters, piezoelectric converters, and biological sensors [18][19][20][21][22][23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

One-pot biosynthesis of CdS quantum dots through in vitro regeneration of hairy roots of Rhaphanus sativus L. And their apoptosis effect on MCF-7 and AGS cancerous human cell lines

Gholami

Dadmehr

Jelodar

et al. 2020

Mater. Res. Express

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Development of green based synthesis of nanoparticles has been regarded as a novel and safe alternative method compared to conventional methods. Semiconductor cadmium sulfide quantum dots (CdS QDs) possess unique biological and medical applications includes labeling cells, diagnosing of diseases and imaging intercellular events. The present paper reports the biosynthesis of CdS QDs through aqueous extracts of the regenerated hairy roots of Rhaphanus sativus L. as the organic source for both reducing and stabilizing of Cd and S precursor ions. The characterization of synthesized QDs showed maximum absorbance peak of 460 nm and fluorescence spectrum of cadmium sulfide at 530 nm. The results of Transmission Electron Microscope (TEM) and EDS analysis demonstrated that the particles were morphologically spherical with size distribution between 2-7 nm and confirmed presence of CdS QDs. Fourier transform infrared spectroscopy (FT-IR) also showed the active presence of aromatic, amino, and carboxyl groups on the surface of quantum dots. Cytotoxicity effect of the synthesized CdS QDs on two cell lines include MCF-7 breast cancer and AGS gastric cancer were assayed through MTT assay. The results showed significant inhibitory effects of synthesized QDs on treated cells in a dose dependent manner. It was also concluded that CdS QDs had more apoptosis effect on MCF-7 cells rather than AGS cell lines. The obtained results clearly illustrated that the synthesis of CdS quantum dots with standard features would be possible through costeffective, reliable, environmentally friendly and less toxic alternative method compared to chemical and physical processes and the MTT toxicity assay also illustrated the significant apoptotic effects of synthesized CdS QDs on carcinogenesis.

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An approach toward miRNA detectionviadifferent thermo-responsive aggregation/disaggregation of CdTe quantum dots

Abstract: A novel and “light shift” spectral method for the detection of miRNA based on different thermal-responsive aggregation/disaggregation of CdTe quantum dots was investigated.

Cited by 17 publications

References 45 publications

Building Polyvalent DNA‐Functionalized Anisotropic AuNPs using Poly‐Guanine‐Mediated In‐Situ Synthesis for LSPR‐Based Assays: Case Study on OncomiR‐155

Building Polyvalent DNA‐Functionalized Anisotropic AuNPs using Poly‐Guanine‐Mediated In‐Situ Synthesis for LSPR‐Based Assays: Case Study on OncomiR‐155

Exploitation of N-Gene of SARS-CoV-2 to Develop a New Rapid Assay by ASOs@AuNPs

One-pot biosynthesis of CdS quantum dots through in vitro regeneration of hairy roots of Rhaphanus sativus L. And their apoptosis effect on MCF-7 and AGS cancerous human cell lines

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