An aptamer array for discriminating tetracycline antibiotics based on binding-enhanced intrinsic fluorescence

Zhao, Yichen; Gao, Biwen; Chen, Yijing; Liu, Juewen

doi:10.1039/d3an00154g

Cited by 12 publications

(13 citation statements)

References 46 publications

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“…However, a slightly weaker ECL response would be witnessed when detecting tetracycline compared with the signal for oxytetracycline. This is because tetracycline and oxytetracycline have similar structures, and the aptamer would have a certain affinity for them [36]. The significant disparity in response signals to tetracycline and oxytetracycline exhibited by the ECL sensor was substantial for subsequent principal component analysis (PCA), facilitating the discrimination of oxytetracycline (OTC) and tetracycline (TC).…”

Section: Characterization Analysis Of Ecl Aptasensormentioning

confidence: 99%

A perovskite‐based electrochemiluminescence aptasensor for tetracycline screening

Chen,

Zhong,

Yang

et al. 2024

Luminescence

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Tetracyclines are currently the most commonly used class of antibiotics, and their residue issue significantly impacts public health safety. In this study, a surface modification of perovskite with cetyltrimethylammonium bromide led to the generation of stable electrochemiluminescence (ECL) emitters in aqueous systems and improved the biocompatibility of perovskite. A perovskite quantum dot‐based ECL sensing strategy was developed. Utilizing the corresponding aptamer of the antibiotics, strain displacement reactions were triggered, disrupting the ECL quenching system composed of perovskite and Ag nanoclusters (Ag NCs) on the electrode surface, generating a signal to achieve quantitative detection of several common tetracycline antibiotics. The perovskite quantum dot provided a strong and stable initial signal, while the efficient catalytic activity of the silver cluster enhanced the recognition sensitivity. Tetracycline, chlortetracycline, and oxytetracycline were used as examples to demonstrate the differentiation and quantitative detection through this method. In addition, the aptasensor exhibited analytical performance with the linear range (0.1–10 μM OTC) and good recovery rates of 94.7% to 101.6% in real samples. This approach has the potential to become a sensitive and practical approach for assessing antibiotic residues.

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Section: Characterization Analysis Of Ecl Aptasensormentioning

confidence: 99%

A perovskite‐based electrochemiluminescence aptasensor for tetracycline screening

Chen,

Zhong,

Yang

et al. 2024

Luminescence

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“…[11] The presence of so many different aptamers for OTC could be related to the relatively large structure of OTC, allowing different ways of aptamer binding. The following studies aims to understand why the fluorescence enhancement of the [11] with permission. Copyright Royal Society of Chemistry 2023.…”

Section: The Ten Oxytetracycline Binding Aptamersmentioning

confidence: 99%

“…[9][10] We recently reported a number of DNA aptamers for tetracycline antibiotics that could all enhance the intrinsic fluorescence of the antibiotics. [11][12] This fluorescence property allowed us to explore a new method for their detection and for fundamental binding assays. [13][14][15][16] Only the fluorescence of tetracyclines could be enhanced by adding the aptamers.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the Binding Properties of Ten Aptamers Using the Intrinsic Fluorescence of Oxytetracycline

Zhao,

Gao,

Liu

2024

ChemistryOpen

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Tetracyclines are a class of commonly used four‐ringed antibiotics. A series of DNA aptamers were recently obtained using the capture‐SELEX (systematic evolution of ligands by exponential enrichment) method to bind to oxytetracycline, and one of the aptamers can bind to a few other tetracycline antibiotics as well. Upon binding to the aptamers, the intrinsic fluorescence of tetracycline antibiotics can be enhanced. At least 10 different DNA aptamers were isolated from the previous selection experiment. In this work, a systematic characterization of these ten aptamers was performed. Each of these aptamers shows a different degree of fluorescence enhancement ranging from around 1‐fold to over 20‐fold. Fluorescence enhancement was boosted in the presence of Mg2+. Isothermal titration calorimetry (ITC) studies were done and showed a great variety in dissociation constant (Kd) from 62 nM to 1.6 μM. Steady‐state fluorescence spectroscopy and fluorescence lifetime studies showed a correlation between fluorescence lifetime and degree of fluorescence enhancement. A few aptamers showed slow binding kinetics, although no correlation was found between the kinetics of fluorescence change and degree of fluorescence enhancement. This is the first study of ten different aptamers for the same target, providing fundamental insights into aptamer binding and bioanalytical applications.

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“…One such example is the OTC5 aptamer that can bind to tetracycline antibiotics. 22,25,26 OTC5 has a similar binding to a few tetracycline antibiotics. In this work, using fluorescence spectroscopy and isothermal titration calorimetry (ITC), we measured both the thermodynamic and kinetic properties of the full-length and split OTC5 aptamers.…”

Section: Introductionmentioning

confidence: 99%

Light-up split aptamers: binding thermodynamics and kinetics for sensing

Zhao,

Patel,

Sun

et al. 2023

Analyst

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An aptamer array for discriminating tetracycline antibiotics based on binding-enhanced intrinsic fluorescence

Abstract: Tetracyclines are a class of antibiotics with a similar four-ringed structure. Due to this structural similarity, they are not easily differentiated from each other. We recently selected aptamers using oxytetracycline...

Cited by 12 publications

References 46 publications

A perovskite‐based electrochemiluminescence aptasensor for tetracycline screening

A perovskite‐based electrochemiluminescence aptasensor for tetracycline screening

Characterization of the Binding Properties of Ten Aptamers Using the Intrinsic Fluorescence of Oxytetracycline

Light-up split aptamers: binding thermodynamics and kinetics for sensing

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