An aqueous ratiometric fluorescence probe for Zn(II)

Sclafani, Joseph A.; Maranto, M. T.; Sisk, Thomas M.; Arman, Scott A. Van

doi:10.1016/0040-4039(96)00257-2

Cited by 67 publications

(28 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…44 Conjugating these zinc-specific receptors onto fluorescent chromophores such as fluorescein, 32–35,41,42,45–55 coumarin, 56,57 quinoline, 31,58–63 and 4-nitrobenzo-oxadiazole 64–66 has produced a variety of fluorescence turn-on 31,32,47,49,52,63,65–85 and ratiometric sensors. 49,56,61,86–110 Despite their attractive features, fluorescent zinc sensors have some significant drawbacks, especially intrinsic signal contamination by autofluorescence and scattered light, which increases background and diminishes signal fidelity. Although approaches such as NIR emission 111,112 have been pursued, reduction of the background was an unsolved problem.…”

Section: Introductionmentioning

confidence: 99%

Phosphorescent Sensor for Biological Mobile Zinc

You

Lee²,

Kim³

et al. 2011

J. Am. Chem. Soc.

226

128

View full text Add to dashboard Cite

A new phosphorescent zinc sensor (ZIrF) was constructed based on an Ir(III) complex bearing two 2-(2,4-difluorophenyl)pyridine (dfppy) cyclometalating ligands and a neutral 1,10-phenanthroline (phen) ligand. A zinc-specific di(2-picolyl)amino (DPA) receptor was introduced at the 4-position of the phen ligand via a methylene linker. The cationic Ir(III) complex exhibited dual phosphorescence bands in CH3CN solutions originating from blue and yellow emission of the dfppy and phen ligands, respectively. Zinc coordination selectively enhanced the latter, affording a phosphorescence ratiometric response. Electrochemical techniques, quantum chemical calculations, and steady-state and femtosecond spectroscopy were employed to establish a photophysical mechanism for this phosphorescence response. The studies revealed that zinc coordination perturbs nonemissive processes of photoinduced electron transfer (PeT) and intraligand charge transfer (ILCT) transition occurring between DPA and phen. ZIrF can detect zinc ions in a reversible and selective manner in buffered solution (pH 7.0, 25 mM PIPES) with Kd = 11 nM and pKa = 4.16. Enhanced signal-to-noise ratios were achieved by time-gated acquisition of long-lived phosphorescence signals. The sensor was applied to image biological free zinc ions in live A549 cells by confocal laser scanning microscopy. A fluorescence lifetime imaging microscope (FLIM) detected an increase in photoluminescence lifetime for zinc-treated A549 cells as compared to controls. ZIrF is the first successful phosphorescent sensor that detects zinc ions in biological samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Phosphorescent Sensor for Biological Mobile Zinc

You

Lee²,

Kim³

et al. 2011

J. Am. Chem. Soc.

226

128

View full text Add to dashboard Cite

show abstract

“…The chelating bichromophores that bear electron-donating atoms in the interlinking chain undergo a conformational change upon complexation with metal ions, and to such a change responds the excimer emission sensitively [13][14][15][16][17][18][19][20][21][22]. The origin of this emission characteristic is based on monomer-excimer interconversion, and is different from that reported more commonly for monochromophoric sensors in which a change in fluorescence is caused by direct coordination on the conjugated system or by photo-induced electron transfer (PET) between the coordination site and emission unit [23][24][25][26][27][28].…”

mentioning

confidence: 99%

Cd2+-sensiting bichromophore: Excimer emission from an EDTA-methylnaphthalene derivative

Machi

Santacruz‐Ortega

Sánchez

et al. 2007

Inorganic Chemistry Communications

View full text Add to dashboard Cite

“…A specific interaction between the tether and an analyte of interest could be created to afford a ratiometric indicator, in which the interaction between the analyte and the tether receptor changes the emission ratio of excimer/exciplex and monomer. 124–129 …”

Section: Singlet State Ligand-centered Photophysical Processesmentioning

confidence: 99%

Zn(ii)-coordination modulated ligand photophysical processes – the development of fluorescent indicators for imaging biological Zn(ii) ions

et al. 2014

View full text Add to dashboard Cite

Molecular photophysics and metal coordination chemistry are the two fundamental pillars that support the development of fluorescent cation indicators. In this article, we describe how Zn(II)-coordination alters various ligand-centered photophysical processes that are pertinent to developing Zn(II) indicators. The main aim is to show how small organic Zn(II) indicators work under the constraints of specific requirements, including Zn(II) detection range, photophysical requirements such as excitation energy and emission color, temporal and spatial resolutions in a heterogeneous intracellular environment, and fluorescence response selectivity between similar cations such as Zn(II) and Cd(II). In the last section, the biological questions that fluorescent Zn(II) indicators help to answer are described, which have been motivating and challenging this field of research.

show abstract

An aqueous ratiometric fluorescence probe for Zn(II)

Cited by 67 publications

References 22 publications

Phosphorescent Sensor for Biological Mobile Zinc

Phosphorescent Sensor for Biological Mobile Zinc

Cd2+-sensiting bichromophore: Excimer emission from an EDTA-methylnaphthalene derivative

Zn(ii)-coordination modulated ligand photophysical processes – the development of fluorescent indicators for imaging biological Zn(ii) ions

Contact Info

Product

Resources

About