An arginine-dependent step in the maturation of type 2 adenovirus

Rouse, Harriet; Schlesinger, R. Walter

doi:10.1016/0042-6822(67)90128-6

Cited by 78 publications

(21 citation statements)

References 28 publications

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“…Noteworthy, arginine had been demonstrated as absolutely necessary for adenovirus production (Bonifas, 1967;Clark Rouse and Schlesinger, 1967;Heilman and Rouse, 1980;Kumel and Hammer, 1979;Wigand and Kumel, 1978). Clark Rouse and Schlesinger, (1967) had proposed earlier that arginine played a specific role in a late synthetic step for virion maturation. Without arginine, there was no virion production, but if arginine was added at 28-32 h after infection, the virion production was restored.…”

Section: Discussionmentioning

confidence: 98%

293SF Metabolic Flux Analysis during Cell Growth and Infection with an Adenoviral Vector

Nadeau

Jacob²,

Perrier

et al. 2000

Biotechnol. Prog.

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Metabolic flux quantification of cell culture is becoming a crucial means to improve cell growth as well as protein and vector productions. The technique allows rapid determination of cell culture status, thus providing a tool for further feeding improvements. Herein, we report on key results of a metabolic investigation using 293 cells adapted to suspension and serum-free medium (293SF) during growth and infection with an adenoviral vector encoding the green fluorescence protein (GFP). The model developed contains 35 fluxes, which include the main fluxes of glycolysis, glutaminolysis, and amino acids pathways. It requires specific consumption and production rate measurements of amino acids, glucose, lactate, NH(3), and O(2), as well as DNA and total proteins biosynthesis rate measurements. Also, it was found that extracellular protein concentration measurement is important for flux calculation accuracy. With this model, we are able to describe the 293SF cell metabolism, grown under different culture conditions in a 3-L controlled bioreactor for batch and fed-batch with low glucose. The metabolism is also investigated during infection under two different feeding strategies: a fed-batch starting at the end of the growth phase and extending during infection without medium change and a fed-batch after infection following medium renewal. Differences in metabolism are observed between growth and infection, as well as between the different feeding strategies, thus providing a better understanding of the general metabolism.

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Section: Discussionmentioning

confidence: 98%

293SF Metabolic Flux Analysis during Cell Growth and Infection with an Adenoviral Vector

Nadeau

Jacob²,

Perrier

et al. 2000

Biotechnol. Prog.

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“…The growth of adenoviruses is dependent on arginine more than on any other single amino acid (12,13,18). Synthesis of herpes virus, SV40, polyoma and reovirus also appear to require arginine for production of infectious progeny (for refs.…”

Section: Introductionmentioning

confidence: 98%

On the mechanism of arginine requirement for adenovirus synthesis

Plaat

Weber

1979

Archives of Virology

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The effects of arginine deprivation on the synthesis and processing of viral proteins and the assembly of incomplete and complete virions were studied during infection with human adenovirus type 2. Arginine deprivation greatly reduced the synthesis of all viral proteins, particularly the precursor to core protein VII. The inhibition was completely reversible by the addition of arginine to the medium. Arginine deprivation between 7 and 20 hours post-infection inhibited the processing of PVII to VII, suggesting that PVII is not cleaved autocatalytically. The assembly of incomplete virions was sensitive to arginine deprivation only prior to 20 hours, while the assembly of complete virions was dependent on the continuous presence of arginine. This observation supports the hypothesis that incomplete virions are precursors of complete virions. The experiments on the PVII-specific endoprotease activity showed that arginine deprivation caused only slight reduction in the in vitro activity, although no activity was observed in vivo. The present results lead to the hypothesis that arginine deficiency inhibits the synthesis of a functional protein essential for virion maturation, other than the synthesis or processing of PVII.

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“…In addition, the presence of arginine is also required for a late function involving virus assembly (Archard & Williamson,197I). In contrast, polyoma, adeno, pseudorabies and herpes simplex viruses, viruses which mature in the cell nucleus, synthesized both virus DNA and protein in arg-cells (Becker et al I967;Rouse & Schlesinger, 1967;Russel & Becker, I968;Olshevsky & Becker, 197o;Courtney, McCombs & Benyesh-Melnick, I97I;Mark & Kaplan, I97I;Winters & Consigli, I97I). In cells infected with these viruses, a late virus function(s) was affected in the absence of arginine, resulting in either the failure of transport of virus proteins synthesized in the cytoplasm into the nucleus or, once inside the nucleus, a lack of assembly of virus proteins and DNA into infectious particles.…”

Section: Introductionmentioning

confidence: 99%

“…Cells infected with a variety of viruses containing DNA such as adenovirus (Rouse & Schlesinger, 1967;Russell & Becker, I968), simian virus 40 (SV4o) (Goldblum,Ravid & Becker,i968), polyoma (Winters & Consigli, I97I), vaccinia (Archard & Williamson,197 I), pseudorabies (Mark & Kaplan,197 I) and herpes simplex viruses (Tankersley, 1964;Becker, Olshevsky & Levitt, 1967;Inglis, 1968;Courtney, McCombs & Benyesh-Melnick, I97O) failed to yield infectious virus when maintained in culture medium lacking arginine. In the case of vaccinia virus, which replicates and matures in the cell cytoplasm, the inhibition of virus yield in arginine-deprived (arg-) cells resulted from a lack of both virus specific RNA (early virus function) and virus DNA synthesis.…”

Section: Introductionmentioning

confidence: 99%

Replication of Simian Virus 40 in Permissive Cells: assembly of Virus Components

Tan

Sokol

1974

Journal of General Virology

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SUMMARYIncubation in medium lacking arginine of monkey kidney cells infected with simian virus 40 resulted in a marked inhibition of production of both infectious virus and empty capsids. Virus DNA synthesis and formation of a virus DNAprotein complex were unaffected. All the virus structural polypeptides were made, although in reduced amounts (42 %) when compared with infected cells incubated in medium containing arginine. Protein synthesis was inhibited in uninfected cells deprived of arginine. All the newly synthesized virus proteins were found in the nuclei of arginine-deprived cells. Addition of arginine to arginine-deprived cells resulted in the assembly of pre-synthesized virus components into infectious virus and empty capsids, as well as the production of virus from virus proteins and DNA synthesized after the addition of arginine. Studies with inhibitors of protein and DNA synthesis suggested that two late virus functions were affected in the absence of arginine: the assembly of capsids and the encapsidation of DNA by capsids to form infectious virus.

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An arginine-dependent step in the maturation of type 2 adenovirus

Cited by 78 publications

References 28 publications

293SF Metabolic Flux Analysis during Cell Growth and Infection with an Adenoviral Vector

293SF Metabolic Flux Analysis during Cell Growth and Infection with an Adenoviral Vector

On the mechanism of arginine requirement for adenovirus synthesis

Replication of Simian Virus 40 in Permissive Cells: assembly of Virus Components

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