An array-based electrochemical magneto-immunosensor for early neonatal sepsis diagnostic: Fast and accurate determination of C-reactive protein in whole blood and plasma samples

Molinero-Fernández, Águeda; Moreno‐Guzmán, María; López, Miguel Ángel; Escarpa, Alberto

doi:10.1016/j.microc.2020.104913

Cited by 9 publications

(1 citation statement)

References 37 publications

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“…The classic case is commercial blood glucose meters for glucose determination [24]. Particularly, electrochemical immunosensors use antibodies or antigens as biomolecular recognition element components placed on the electrode surface, the formation of stable immunological complexes to generate an electrical signal, and the acquisition and output of electrochemical signals to complete the target detection (proteins, bacteria, viruses, or small molecules) [25,26]. The constructed electrochemical immunosensor has the advantages of both immunosensor and electrochemical techniques.…”

Section: Introductionmentioning

confidence: 99%

The Applications of Electrochemical Immunosensors in the Detection of Disease Biomarkers: A Review

et al. 2023

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Disease-related biomarkers may serve as indicators of human disease. The clinical diagnosis of diseases may largely benefit from timely and accurate detection of biomarkers, which has been the subject of extensive investigations. Due to the specificity of antibody and antigen recognition, electrochemical immunosensors can accurately detect multiple disease biomarkers, including proteins, antigens, and enzymes. This review deals with the fundamentals and types of electrochemical immunosensors. The electrochemical immunosensors are developed using three different catalysts: redox couples, typical biological enzymes, and nanomimetic enzymes. This review also focuses on the applications of those immunosensors in the detection of cancer, Alzheimer’s disease, novel coronavirus pneumonia and other diseases. Finally, the future trends in electrochemical immunosensors are addressed in terms of achieving lower detection limits, improving electrode modification capabilities and developing composite functional materials.

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Section: Introductionmentioning

confidence: 99%

The Applications of Electrochemical Immunosensors in the Detection of Disease Biomarkers: A Review

et al. 2023

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Affinity-based electrochemical sensors for biomolecular detection in whole blood

Wilkirson

Singampalli

et al. 2023

Anal Bioanal Chem

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The detection and/or quantification of biomarkers in blood is important for the early detection, diagnosis, and treatment of a variety of diseases and medical conditions. Among the different types of sensors for detecting molecular biomarkers, such as proteins, nucleic acids, and small-molecule drugs, affinity-based electrochemical sensors offer the advantages of high analytical sensitivity and specificity, fast detection times, simple operation, and portability. However, biomolecular detection in whole blood is challenging due to its highly complex matrix, necessitating sample purification (i.e., centrifugation), which involves the use of bulky, expensive equipment and tedious sample-handling procedures. To address these challenges, various strategies have been employed, such as purifying the blood sample directly on the sensor, employing micro-/nanoparticles to enhance the detection signal, and coating the electrode surface with blocking agents to reduce nonspecific binding, to improve the analytical performance of affinity-based electrochemical sensors without requiring sample pre-processing steps or laboratory equipment. In this article, we present an overview of affinity-based electrochemical sensor technologies that employ these strategies for biomolecular detection in whole blood. Graphical abstract

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Micro- and nanosensors for detecting blood pathogens and biomarkers at different points of sepsis care

et al. 2022

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Severe infections can cause a dysregulated response leading to organ dysfunction known as sepsis. Sepsis can be lethal if not identified and treated right away. This requires measuring biomarkers and pathogens rapidly at the different points where sepsis care is provided. Current commercial approaches for sepsis diagnosis are not fast, sensitive, and/or specific enough for meeting this medical challenge. In this article, we review recent advances in the development of diagnostic tools for sepsis management based on micro- and nanostructured materials. We start with a brief introduction to the most popular biomarkers for sepsis diagnosis (lactate, procalcitonin, cytokines, C-reactive protein, and other emerging protein and non-protein biomarkers including miRNAs and cell-based assays) and methods for detecting bacteremia. We then highlight the role of nano- and microstructured materials in developing biosensors for detecting them taking into consideration the particular needs of every point of sepsis care (e.g., ultrafast detection of multiple protein biomarkers for diagnosing in triage , emergency room, ward, and intensive care unit; quantitative detection to de-escalate treatment; ultrasensitive and culture-independent detection of blood pathogens for personalized antimicrobial therapies; robust, portable, and web-connected biomarker tests outside the hospital). We conclude with an overview of the most utilized nano- and microstructured materials used thus far for solving issues related to sepsis diagnosis and point to new challenges for future development. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-022-05171-2.

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An array-based electrochemical magneto-immunosensor for early neonatal sepsis diagnostic: Fast and accurate determination of C-reactive protein in whole blood and plasma samples

Cited by 9 publications

References 37 publications

The Applications of Electrochemical Immunosensors in the Detection of Disease Biomarkers: A Review

The Applications of Electrochemical Immunosensors in the Detection of Disease Biomarkers: A Review

Affinity-based electrochemical sensors for biomolecular detection in whole blood

Micro- and nanosensors for detecting blood pathogens and biomarkers at different points of sepsis care

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