An Assessment of the Value of Serum Cholinesterase as a Liver Function Test and in the Diagnosis of Jaundice

Burnett, W.

doi:10.1136/gut.1.4.294

Cited by 18 publications

(11 citation statements)

References 14 publications

(11 reference statements)

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“…AFP, the only clinically used marker for HCC diagnosis, was only detected in HCC serum. Cholinesterase, formed in liver, is widely measured as a liver function test and the activity was reported to decrease in liver cirrhosis or hepatocellular carcinoma (27)'which is in accordance with our results. Vitronectin, which is a multifunctional plasma glycoprotein that participates in the regulation of coagulation, fibrinolysis, and the complement cascade (28), was also found in decreased concentration in serum of HCC patients.…”

Section: Resultssupporting

confidence: 91%

Development of Glycoprotein Capture-Based Label-Free Method for the High-throughput Screening of Differential Glycoproteins in Hepatocellular Carcinoma

Chen

Tan

Wang

et al. 2011

Molecular & Cellular Proteomics

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A robust, reproducible, and high throughput method was developed for the relative quantitative analysis of glycoprotein abundances in human serum. Instead of quantifying glycoproteins by glycopeptides in conventional quantitative glycoproteomics, glycoproteins were quantified by nonglycosylated peptides derived from the glycoprotein digest, which consists of the capture of glycoproteins in serum samples and the release of nonglycopeptides by trypsin digestion of captured glycoproteins followed by two-dimensional liquid chromatography-tandem MS analysis of released peptides. Protein quantification was achieved by comparing the spectrum counts of identified nonglycosylated peptides of glycoproteins between different samples. This method was demonstrated to have almost the same specificity and sensitivity in glycoproteins quantification as capture at glycopeptides level. The differential abundance of proteins present at as low as nanogram per milliliter levels was quantified with high confidence. The established method was applied to the analysis of human serum samples from healthy people and patients with hepatocellular carcinoma (HCC) to screen differential glycoproteins in HCC. Thirty eight glycoproteins were found with substantial concentration changes between normal and HCC serum samples, including ␣-fetoprotein, the only clinically used marker for HCC diagnosis. The abundance changes of three glycoproteins, i.e. galectin-3 binding protein, insulin-like growth factor binding protein 3, and thrombospondin 1, which were associated with the development of HCC, were further confirmed by enzyme-linked immunosorbent assay. In conclusion, the developed method was an effective approach to quantitatively analyze glycoproteins in human serum and could be further applied in the biomarker discovery for HCC and other cancers. Molecular

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Section: Resultssupporting

confidence: 91%

Development of Glycoprotein Capture-Based Label-Free Method for the High-throughput Screening of Differential Glycoproteins in Hepatocellular Carcinoma

Chen

Tan

Wang

et al. 2011

Molecular & Cellular Proteomics

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“…The SGOT to SGPT ratio in our study was 0.84 which may be exhibited mild chronic liver disease. Serum cholinesterase is useful both as a liver function test and in the diagnosis of jaundice provided consideration is given to the other factors which affect the level of activity of the enzyme [24]. Even though, all blood parameters were in normal ranges at present study but it should be recommended that the monitoring of BuChE, Hb, Hct, BUN, Cr, SGOT and SGPT should be continued for a longer period of time in order to observe the biological changes of chronic diseases in occupational workers who directly expose to the VOC, such as the gasoline station workers.The BuChE may be considered as a potential biomarker of adverse health effects of red blood cell, kidney and liver function and skin irritation from VOC exposure.…”

Section: Discussionmentioning

confidence: 99%

The Correlative Study of Serum Pseudo-cholinesterase, Biological Parameters and Symptoms Among Occupational Workers

2013

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Cholinesterase is one of many important enzymes needed for acetylcholine hydrolysis. This study aimed to evaluate the serum pseudo-cholinesterase (BuChE) level and its relation to biological parameters (hematological and biochemical parameters) and symptoms among occupational workers, gasoline station worker in Bangkok, Thailand. Our results revealed that the average BuChE level was in normal range, but it had strong relation to hemoglobin (Hb), hematocrit (Hct), blood urea nitrogen (BUN), creatinine (Cr) and serum glutamic pyruvic transaminase (SGPT) (Linear regression analysis, p \ 0.05, p \ 0.05, p \ 0.01, p = 0

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“…As part of the standard routine blood profile captured in the supine position under resting and fasting conditions, creatinine, blood urea nitrogen, serum albumin, aspartate and alanine amino transferase levels were assessed. PCHE activity, a serum marker of hepatic metabolizing capacity, 13,14 was obtained in a subgroup of 21 patients using photometry (normal range for patients of at least 16 years of age: 5860-13,000 U/L). The study was in accordance with the institutional guidelines.…”

Section: Methodsmentioning

confidence: 99%

From Heart Failure to Highly Unsaturated Fatty Acid Deficiency and Vice Versa: Bidirectional Heart and Liver Interactions

Alter

Glück

Figiel

et al. 2016

Canadian Journal of Cardiology

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An Assessment of the Value of Serum Cholinesterase as a Liver Function Test and in the Diagnosis of Jaundice

Cited by 18 publications

References 14 publications

Development of Glycoprotein Capture-Based Label-Free Method for the High-throughput Screening of Differential Glycoproteins in Hepatocellular Carcinoma

Development of Glycoprotein Capture-Based Label-Free Method for the High-throughput Screening of Differential Glycoproteins in Hepatocellular Carcinoma

The Correlative Study of Serum Pseudo-cholinesterase, Biological Parameters and Symptoms Among Occupational Workers

From Heart Failure to Highly Unsaturated Fatty Acid Deficiency and Vice Versa: Bidirectional Heart and Liver Interactions

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