An Audit and Comparison of pH, Measured Concentration, and Particulate Matter in Mannitol and Hypertonic Saline Solutions

Carr, Christopher J.; Scoville, Jonathan; Ruble, James; Condie, Chad; Davis, George K.; Floyd, Candace L.; Kelly, Logan; Monson, Kenneth L.; Reichert, Ethan C.; Sarigul, Buse; Hawryluk, Gregory

doi:10.3389/fneur.2021.667842

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…A refractometer (Autoutlet, China) with a precision of ±1 in practical salinity scale was used to measure salinity [33]. No-salt ASN-III media was used to calibrate the refractometer.…”

Section: Salinity Assessmentmentioning

confidence: 99%

Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application on Saline Wastewater Treatment and Biomass Production

Tanoeiro,

Fehrenbach,

Murray

et al. 2024

Preprint

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This study investigated the adaptability of Dunaliella salina to different salinity levels, with emphasis on growth, pigment concentration, and desalination potential. It was found that among the 21 salinity levels, salinity 75 produced consistently favorable results in cell count (13.08 x 103 ± 1.41 x 103 cells/mL), dry biomass (2.46 ± 0.06 g/L), pigment content (chlorophyll a = 97.5 ± 0.1 µg/L, chlorophyll b = 123.6 ± 0.3 µg/L), and desalination (9.32 ± 0.47 reduction). Therefore, salinity 75 was selected for the final trial (scale-up), which revealed unanticipatedly high cell counts (58.96 x 103 ± 535.22 cells/mL), with dry biomass weight being statistically different (higher) than the expected (4.21 ± 0.02 g/L) (p<0.0001), most likely due to high cell count and energy reserve storage for high salinity adaption in the form of bio-compounds. Pigment growth continued (chlorophyll a = 95.4 ± 2.2 µg/L, chlorophyll b = 128.1 ± 5.1 µg/L), indicating pigment production under salt stress. Notably, desalination did not occur in this stage, possibly due to the necessity for a bigger initial inoculate, prolonged exposure or bioaccumulation becoming the prevailing mechanism over desalination. Nevertheless, the trial highlights D. salina's strong adaptation to various salinity levels. This suggests a promising future in halophyte research, particularly in understanding the mechanisms that prevent salt accumulation in cells and how to overcome this barrier. Additionally, these results suggest that microalgae could be a viable resource in saline-rich environments unsuitable for conventional agriculture, promoting industrial adaptation to adverse conditions.

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“…A refractometer (Autoutlet, China) with a precision of ±1 in practical salinity scale was used to measure salinity [33]. No-salt ASN-III media was used to calibrate the refractometer.…”

Section: Salinity Assessmentmentioning

confidence: 99%

Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application on Saline Wastewater Treatment and Biomass Production

Tanoeiro,

Fehrenbach,

Murray

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…A refractometer (Autoutlet, China) was used to measure salinity [23]. No-salt ASN-III media was used to calibrate the refractometer.…”

Section: Salinity Assessmentmentioning

confidence: 99%

Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application on Saline Wastewater Treatment and Biomass Production

Tanoeiro,

Fehrenbach,

Murray

et al. 2024

Preprint

View full text Add to dashboard Cite

This study investigated the adaptability of Dunaliella salina to different salinity levels, with emphasis on growth, pigment concentration, and desalination potential. It was found that among the 21 salinity levels (0-20%), 75‰ salinity produced consistently favorable results in cell count (13.08 x 103 ± 1.41 x 103 cells/mL), dry biomass (2.46 ± 0.06 g/L), pigment content (chlorophyll a = 9.75 ± 0.01 µg/L, chlorophyll b = 12.36 ± 0.03 µg/L), and desalination (9.32 ± 0.47 % reduction). Therefore, 75‰ salinity level was selected for the final trial (scale-up), which revealed unanticipatedly high cell counts (58.96 x 103 ± 535.22 cells/mL), with dry biomass weight being statistically extremely different (higher) than the expected (4.21 ± 0.02 g/L) (p&lt;0.0001), most likely due to high cell count and energy reserve storage for high salinity adaption in the form of bio-compounds. Pigment growth continued (chlorophyll a = 9.54 ± 0.2 µg/L, chlorophyll b = 12.81 ± 0.51 µg/L), indicating pigment production under salt stress. Notably, desalination did not occur in this stage, possibly due to the necessity for a bigger initial inoculate, prolonged exposure or bioaccumulation becoming the prevailing mechanism over desalination. Despite this, the microalgae thrived in high salinity conditions. These findings point to further study into the mechanics of salt build-up within cells, which might lead to successful desalination technologies. Dunaliella salina adaptation to high salinity settings suggests its possible usage in natural pools or soils that are normally inappropriate for traditional production, giving a chance for enterprises to function in rough extreme salinity conditions.

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Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application in Saline Water Treatment and Biomass Production

Tanoeiro,

Fehrenbach,

Murray

et al. 2024

Aquaculture Journal

View full text Add to dashboard Cite

This study investigated the adaptability of Dunaliella salina to different salinity levels, with an emphasis on growth, pigment concentration, and desalination potential. It was found that among the 21 salinity levels, Salinity 75 produced consistently favorable results in cell count (13.08 × 103 ± 1.41 × 103 cells/mL), dry biomass (2.46 ± 0.06 g/L), pigment content (chlorophyll a = 97,500,000 ± 100,000 pg/L, chlorophyll b = 123,600,000 ± 300,000 pg/L), and desalination (9.32 ± 0.47 reduction). Therefore, Salinity 75 was selected for the final trial (scale-up), which revealed unanticipatedly high cell counts (58.96 × 103 ± 535.22 cells/mL), with the dry biomass weight being statistically different (higher) than expected (4.21 ± 0.02 g/L) (p < 0.0001), most likely due to the high cell count and energy reserve storage for high-salinity adaption in the form of bio-compounds. Pigment growth continued (chlorophyll a = 95,400,000 ± 2,200,000 pg/L, chlorophyll b = 128,100,000 ± 5,100,000 pg/L), indicating pigment production under salt stress. Notably, desalination did not occur in this stage, possibly due to the necessity for a bigger initial inoculate, prolonged exposure or bioaccumulation becoming the prevailing mechanism over desalination. Nevertheless, the trial highlights D. salina’s strong adaptation to various salinity levels. This suggests a promising future in halophyte research, particularly in understanding the mechanisms that prevent salt accumulation in cells and how to overcome this barrier. Additionally, these results suggest that microalgae could be a viable resource in saline-rich environments unsuitable for conventional agriculture, promoting industrial adaptation to adverse conditions.

show abstract

An Audit and Comparison of pH, Measured Concentration, and Particulate Matter in Mannitol and Hypertonic Saline Solutions

Cited by 3 publications

References 46 publications

Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application on Saline Wastewater Treatment and Biomass Production

Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application on Saline Wastewater Treatment and Biomass Production

Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application on Saline Wastewater Treatment and Biomass Production

Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application in Saline Water Treatment and Biomass Production

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