An automated do-it-yourself system for dynamic stem cell and organoid culture in standard multi-well plates

Tischler, Julia; Swank, Zoe; Hsiung, Hao-An; Vianello, Stefano; Maerkl, Sebastian J.

doi:10.1016/j.crmeth.2022.100244

Cited by 9 publications

(4 citation statements)

References 45 publications

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“…Importantly, the embedding matrix medium limits sample handling and preparation for correlative studies across scales and imaging modalities. New culturing methods for higher throughput 65 in a Matrigel-free environment 66 will further streamline the integration of multiple imaging methods. 3D cell cultures further entail poorer ultrastructural preservation following freezing.…”

Section: Discussionmentioning

confidence: 99%

Light and electron microscopy continuum-resolution imaging of 3D cell cultures

D’Imprima¹,

Montero²,

Gawrzak³

et al. 2023

Developmental Cell

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Section: Discussionmentioning

confidence: 99%

Light and electron microscopy continuum-resolution imaging of 3D cell cultures

D’Imprima¹,

Montero²,

Gawrzak³

et al. 2023

Developmental Cell

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“…In the field of do-it-yourself (DIY) automation are some publications from scientists who share their experiences and results of their own automation systems [ 47 – 51 ]. For scientists who are new to the field of laboratory automation, it is certainly not advisable to try to implement DIY systems in this early stage.…”

Section: Diy and Tailor-made Laboratory Automationmentioning

confidence: 99%

Can I benefit from laboratory automation? A decision aid for the successful introduction of laboratory automation

Rupp,

Ries,

Wienbruch

et al. 2023

Anal Bioanal Chem

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The large volumes of samples to be analysed every day would be impossible to manage without laboratory automation. As laboratory procedures have progressed, so have the tasks of laboratory personnel. With this feature article, we would like to provide (bio)chemical practitioners with little or no knowledge of laboratory automation with a guide to help them decide whether to implement laboratory automation and find a suitable system. Especially in small- and medium-sized laboratories, operating a laboratory system means having bioanalytical knowledge, but also being familiar with the technical aspects. However, time, budget and personnel limitations allow little opportunity for personnel to get into the depths of laboratory automation. This includes not only the operation, but also the decision to purchase an automation system. Hasty investments do not only result in slow or non-existent cost recovery, but also occupy valuable laboratory space. We have structured the article as a decision tree, so readers can selectively read chapters that apply to their individual situation. This flexible approach allows each reader to create a personal reading flow tailored to their specific needs. We tried to address a variety of perspectives on the topic, including people who are either supportive or sceptical of laboratory automation, personnel who want or need to automate specific processes, those who are unsure whether to automate and those who are interested in automation but do not know which areas to prioritize. We also help to make a decision whether to reactivate or discard already existing and unused laboratory equipment.

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“…Despite its widespread usage, to our knowledge, no instrumentation exists that fully automates sample handling and sorting, which greatly limits the ability to sequentially handle large numbers of samples. This contrasts with other workflows where automation has revolutionized basic and applied biological research practices [14][15][16][17][18][19][20]. For example, liquid handling equipment can assist in cell maintenance and treatment, enabling screening campaigns on the order of 10,000 to 100,000 samples per day [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

An open-source FACS automation system for high-throughput cell biology

Wiener,

Huynh,

Jeyakumar

et al. 2024

PLoS ONE

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Recent advances in gene editing are enabling the engineering of cells with an unprecedented level of scale. To capitalize on this opportunity, new methods are needed to accelerate the different steps required to manufacture and handle engineered cells. Here, we describe the development of an integrated software and hardware platform to automate Fluorescence-Activated Cell Sorting (FACS), a central step for the selection of cells displaying desired molecular attributes. Sorting large numbers of samples is laborious, and, to date, no automated system exists to sequentially manage FACS samples, likely owing to the need to tailor sorting conditions (“gating”) to each individual sample. Our platform is built around a commercial instrument and integrates the handling and transfer of samples to and from the instrument, autonomous control of the instrument’s software, and the algorithmic generation of sorting gates, resulting in walkaway functionality. Automation eliminates operator errors, standardizes gating conditions by eliminating operator-to-operator variations, and reduces hands-on labor by 93%. Moreover, our strategy for automating the operation of a commercial instrument control software in the absence of an Application Program Interface (API) exemplifies a universal solution for other instruments that lack an API. Our software and hardware designs are fully open-source and include step-by-step build documentation to contribute to a growing open ecosystem of tools for high-throughput cell biology.

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An automated do-it-yourself system for dynamic stem cell and organoid culture in standard multi-well plates

Cited by 9 publications

References 45 publications

Light and electron microscopy continuum-resolution imaging of 3D cell cultures

Light and electron microscopy continuum-resolution imaging of 3D cell cultures

Can I benefit from laboratory automation? A decision aid for the successful introduction of laboratory automation

An open-source FACS automation system for high-throughput cell biology

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