An automated method for the simultaneous determination of pravastatin, 3-hydroxy isomeric metabolite, pravalactone and fenofibric acid in human plasma by sensitive liquid chromatography combined with diode array and tandem mass spectrometry detection

Mertens, B.; Cahay, Bernard; Klinkenberg, Régis; Streel, Bruno

doi:10.1016/j.chroma.2008.01.060

Cited by 26 publications

(29 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, two LC/MS/MS methods have been developed for determination of pravastatin in human plasma [85,94]. Both methods utilized ESI but in different modes.…”

Section: Liquid Chromatography Coupled To Tandem Mass Spectrometrymentioning

confidence: 99%

“…A sensitive and accurate procedure based on solid-phase extraction coupled at-line to a LC/MS/MS for determination of pravastatin and its two metabolites in human plasma has been presented [85]. Optimized and validated LLE-LC/MS/MS method for determination of pitavastatin and its lacton form in human plasma as well as in urine is described [88].…”

Section: Pharmacokinetic Studiesmentioning

confidence: 99%

“…However, Mertens and co-workers [85] have used two different internal standards for quantification of fenofibric acid, pravastatin and its metabolites in human plasma by automated SPE-LC/DAD/MS/MS technique. To avoid the need for plasma dilution and two time-consuming analytical runs, the use of two internal standards was necessary as the concentration of fenofibric acid was too high and MS signal appeared saturated.…”

Section: Liquid Chromatography Coupled To Tandem Mass Spectrometrymentioning

confidence: 99%

“…To reduce the time of sample preparation, Mertens and co-workers [85] have used an automated SPE on disposable extraction cartridges to isolate pravastatin and its metabolites together with fenofibric acid, another lipid-regulating agent, from the human plasma and to prepare cleaner samples before injection and analysis in the LC/DAD/MS/MS system. Different kinds of disposable extraction cartridges containing bonded silicas of different polarities (ethyl, endcapped ethyl, octyl, endcapped octyl, octadecyl, endcapped octadecyl and cyanopropyl) were tested.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Both methods utilized ESI but in different modes. In the method developed by Martens and co-workers [85] the mass spectrometer was operated in the positive mode. The MS/MS detection was set up in MRM mode.…”

Section: Liquid Chromatography Coupled To Tandem Mass Spectrometrymentioning

confidence: 99%

See 4 more Smart Citations

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

Nigović¹,

Mornar²,

Sertić³

2012

Chromatography - The Most Versatile Method of Chemical Analysis

View full text Add to dashboard Cite

“…Recently, two LC/MS/MS methods have been developed for determination of pravastatin in human plasma [85,94]. Both methods utilized ESI but in different modes.…”

Section: Liquid Chromatography Coupled To Tandem Mass Spectrometrymentioning

confidence: 99%

Section: Pharmacokinetic Studiesmentioning

confidence: 99%

Section: Liquid Chromatography Coupled To Tandem Mass Spectrometrymentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Section: Liquid Chromatography Coupled To Tandem Mass Spectrometrymentioning

confidence: 99%

See 3 more Smart Citations

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

Nigović¹,

Mornar²,

Sertić³

2012

Chromatography - The Most Versatile Method of Chemical Analysis

View full text Add to dashboard Cite

Determination of fenofibric acid in human plasma by ultra performance liquid chromatography–electrospray ionization mass spectrometry: application to a bioequivalence study

Dasandi¹,

Shah²,

Shivprakash³

2009

Biomedical Chromatography

View full text Add to dashboard Cite

A rapid, specific and sensitive ultra-performance liquid chromatography tandem mass spectrometry method was developed for the determination of fenofibric acid in human plasma. The method involves simple, one-step liquid-liquid extraction procedure coupled with an Acquity UPLC(TM) BEH C(18) column (50 x 2.1 mm, i.d., 1.7 microm) with isocratic elution at a flow-rate of 0.2 mL/min and mefenamic acid was used as the internal standard. The Quattro Premier XE mass spectrometry was operated under the multiple reaction-monitoring mode using the electrospray ionization technique. Using 250 microL plasma, the methods were validated over the concentration rang 0.05-7.129 microg/mL, with a lower limit of quantification of 0.05 microg/mL. The intra- and inter-day precision and accuracy were within 9.3%. The recovery was 66.7% and 52.6% for fenofibric acid, and mefenamic acid, respectively. Total run time was 1.8 min only for each sample, which makes it possible to analyze more than 350 samples per day.

show abstract

Development of a sensitive liquid chromatography/tandem mass spectrometry method for the determination of fenofibric acid in rat plasma

Wang

Jizhao²,

Meng³

et al. 2011

Biomedical Chromatography

View full text Add to dashboard Cite

A rapid and sensitive LC-MS/MS method for the quantification of fenofibric acid in rat plasma was developed and validated. Plasma samples were prepared by liquid-liquid extraction with a mixture of N-hexane-dichloromethane-isopropanol (100:50:5, v/v/v). Isocratic chromatographic separation was performed on a reversed-phase Discovery C(18) column (2.1 × 50 mm, 5 µm). The mobile phase was methanol-water-formic (75:25:0.25, v/v/v). Detection of fenofibric acid and the internal standard (IS) diclofenac acid was achieved by ESI MS/MS in the negative ion mode using m/z 317 → m/z 213 and m/z 294 → m/z 250 transitions, respectively. The method was linear from 0.005 to 1.250 µg/mL when 100 μL plasma was analyzed. The lower limit of quantification was 0.005 µg/mL. The intra- and inter-day precision values were below 8.2%, and accuracy ranged from -0.9 to 2.1% in all quality control samples. The recovery was 90.3-94.7% and 83.3% for fenofibric acid and IS, respectively. Total run time for each sample analysis was 2.5 min. The validated method was successfully applied to a pharmacokinetic study in six rats after oral administration of fenofibrate, the ester prodrug of fenofibric acid (equivalent to fenofibric acid 5 mg/kg). The method permits laboratory scientists with access to the appropriate instrumentation to perform rapid fenofibric acid determination.

show abstract

An automated method for the simultaneous determination of pravastatin, 3-hydroxy isomeric metabolite, pravalactone and fenofibric acid in human plasma by sensitive liquid chromatography combined with diode array and tandem mass spectrometry detection

Cited by 26 publications

References 27 publications

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

Determination of fenofibric acid in human plasma by ultra performance liquid chromatography–electrospray ionization mass spectrometry: application to a bioequivalence study

Development of a sensitive liquid chromatography/tandem mass spectrometry method for the determination of fenofibric acid in rat plasma

Contact Info

Product

Resources

About