An automated microscale technique for the quantitative and parallel analysis of microfiltration operations

Jackson, Nigel B.; Liddell, John; Lye, Gary J.

doi:10.1016/j.memsci.2005.09.028

Cited by 51 publications

(47 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It can be seen that the 1 00 UEAV A cartridge performance is within 10% of the 10 00 UEAV cartridge, compared with 41% for the flat-sheet disc. Whilst the 25 mm diameter flat-sheet discs used in these experiments were smaller than the standard 47 mm discs, it was not anticipated that the smaller area would lead to an increase in the degree of variability in performance that have been reported with other small diameter discs (Chandler and Zydney, 2004;Jackson et al, 2006). This was indeed seen to be the case, as the variability in performance was relatively low and did not impact upon the performance prediction that could be made with the flat-sheet disc.…”

Section: Volume Throughput Predictions For Protein Solution Filtrationmentioning

confidence: 86%

See 1 more Smart Citation

Scale‐down prediction of industrial scale pleated membrane cartridge performance

Brown

Titchener‐Hooker

Lye

2010

Biotech & Bioengineering

View full text Add to dashboard Cite

Flat-sheet membrane discs represent the current standard format used for experimental prediction of the scale-up of normal flow filtration processes. Use of this format is problematic, however, since the scale-down results typically show a 40-55% difference in performance compared to large-scale cartridges depending upon the feedstock used. In this work, novel pleated scale-down devices (Am=1.51-15.1×10(-3) m2) have been designed and fabricated. It is shown that these can more accurately predict the performance of industrial scale single-use pleated membrane cartridges (Am=1.06 m2) commonly used within biopharmaceutical manufacture. The single-use scale-down cartridges retain the same pleat characteristics of the larger cartridges, but require a reduced feed volume by virtue of a substantially diminished number of active membrane pleats. In this study, a 1,000-fold reduction in feed volume requirement for the scale-down cartridge with the smallest membrane area was achieved. The scale-down cartridges were tested both with clean water and a pepsin protein solution, showing flux-time relationships within 10% of the large-scale cartridge in both cases. Protein transmission levels were also in close agreement between the different scale cartridges. The similarity in performance of the scale-down and the large-scale cartridges, coupled with the low feed requirement, make such devices an excellent method by which rapid scale-up can be achieved during early stage process development for biopharmaceutical products. This new approach is a significant improvement over using flat-sheet discs as the quantitative similarity in performance with the large-scale leads to reliable scale-up predictions while requiring especially small volumes of feed material.

show abstract

Section: Volume Throughput Predictions For Protein Solution Filtrationmentioning

confidence: 86%

“…Other studies (Chandler and Zydney, 2004;Jackson et al, 2006) have previously shown measurable differences in the performance of membrane taken from different locations within a manufactured roll. Thus it may be expected that reduced pleat levels lead to an increase in experimental variation.…”

Section: Effect Of Number and Distribution Of Membrane Pleats On Perfmentioning

confidence: 94%

Scale‐down prediction of industrial scale pleated membrane cartridge performance

Brown

Titchener‐Hooker

Lye

2010

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…Microwell-based methods are applicable to downstream studies for proteins and are now being published. For example, Jackson et al (2006) have examined an automated microwell-based approach to evaluating microfiltration of E. coli cells. Though a vacuum rather than pressure driven system typical of large-scale was used the predictions were useful in obtaining basic data such as specific cake resistance and the automation allowed a wide ranging study of variables.…”

Section: Microwell-based Studiesmentioning

confidence: 99%

Micro biochemical engineering to accelerate the design of industrial‐scale downstream processes for biopharmaceutical proteins

Titchener‐Hooker

Dunnill

Hoare

2008

Biotech & Bioengineering

View full text Add to dashboard Cite

The article examines how a small set of easily implemented micro biochemical engineering procedures combined with regime analysis and bioprocess models can be used to predict industrial scale performance of biopharmaceutical protein downstream processing. This approach has been worked on in many of our studies of individual operations over the last 10 years and allows preliminary evaluation to be conducted much earlier in the development pathway because of lower costs. It then permits the later large scale trials to be more highly focused. This means that the risk of delays during bioprocess development and of product launch are reduced. Here we draw the outcomes of this research together and illustrate its use in a set of typical operations; cell rupture, centrifugation, filtration, precipitation, expanded bed adsorption, chromatography and for common sources, E. coli, two yeasts and mammalian cells (GS-NSO). The general approach to establishing this method for other operations is summarized and new developments outlined. The technique is placed against the background of the scale-down methods that preceded it and complementary ones that are being examined in parallel. The article concludes with a discussion of the advantages and limitations of the micro biochemical engineering approach versus other methods.

show abstract

“…Flasks, called measuring flasks, for which aeration is accomplished by applying a stream of air instead of a sterile plug, are introduced by Anderlei and Büchs [5] and so far have been used in much research [14][15][16][17]. Many researchers have been working on shake flasks to describe their different characteristics through mathematical models.…”

Section: Introductionmentioning

confidence: 99%

Novel dynamic model for aerated shaking bioreactors

Tabrizi

Amoabediny

Moshiri

et al. 2011

Biotech and App Biochem

View full text Add to dashboard Cite

Abstract.A novel mathematical model of aerated shaking bioreactors is developed and its accuracy is evaluated with experimental data. Corynebacterium glutamicum (DM 1730) and Arxula adeninivorans (WT LS3) are used as model microorganisms. In order to get the experimental data, shake flasks of 250 mL and filling volume of 15 mL, together with online measurement tools for measuring oxygen transfer rate and tools for measuring dry biomass concentration, are used. This mathematical model may be regarded as a key tool for computational simulations or developing model-based control approaches for necessary automation and optimization purposes. Moreover, the error evaluation presented in this paper should be taken into account when using the model. The squared Pearson product-moment correlation coefficient (r 2 ) and average deviation of error are used as two statistical criteria to quantify the accuracy and consistency of the model.

show abstract

An automated microscale technique for the quantitative and parallel analysis of microfiltration operations

Cited by 51 publications

References 31 publications

Scale‐down prediction of industrial scale pleated membrane cartridge performance

Scale‐down prediction of industrial scale pleated membrane cartridge performance

Micro biochemical engineering to accelerate the design of industrial‐scale downstream processes for biopharmaceutical proteins

Novel dynamic model for aerated shaking bioreactors

Contact Info

Product

Resources

About