An automatic centrifugal system for rapid detection of bacteria based on immunomagnetic separation and recombinase aided amplification

Wang, Siyuan; Qi, Wuzhen; Wu, Shangyi; Yuan, Jing; Duan, Hong; Li, Yanbin; Lin, Jianhan

doi:10.1039/d2lc00650b

Cited by 14 publications

(3 citation statements)

References 36 publications

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“…An Android smartphone (K9s, Oppo, Shenzhen, China) equipped with a 64-megapixel camera was utilized to capture and analyze the fluorescence images using a custom-built Android application coded in Java ( Figure 4 b). The RGB values are derived from the region of interest (ROI) in the captured fluorescence images, with the average values calculated to ascertain the fluorescence intensities [ 39 ]. The detailed functionality of the application is presented in Figure S3 .…”

Section: Methodsmentioning

confidence: 99%

An Integrated Microfluidic Biosensing System Based on a Versatile Valve and Recombinase Polymerase Amplification for Rapid and Sensitive Detection of Salmonella typhimurium

Jin

Wang

et al. 2023

Biosensors

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Detecting foodborne pathogens on-site is crucial for ensuring food safety, necessitating the development of rapid, cost-effective, highly sensitive, and portable devices. This paper presents an integrated microfluidic biosensing system designed for the rapid and sensitive detection of Salmonella typhimurium (S. typhimurium). The biosensing system comprises a microfluidic chip with a versatile valve, a recombinase polymerase amplification (RPA) for nucleic acid detection, and a customized real-time fluorescence detection system. The versatile valve combines the functions of an active valve and a magnetic actuation mixer, enabling on-demand mixing and controlling fluid flow. Quantitative fluorescence is processed and detected through a custom-built smartphone application. The proposed integrated microfluidic biosensing system could detect Salmonella at concentrations as low as 1.0 × 102 copies/µL within 30 min, which was consistent with the results obtained from the real-time quantitative polymerase chain reaction (qPCR) tests. With its versatile valve, this integrated microfluidic biosensing system holds significant potential for on-site detection of foodborne pathogens.

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Section: Methodsmentioning

confidence: 99%

An Integrated Microfluidic Biosensing System Based on a Versatile Valve and Recombinase Polymerase Amplification for Rapid and Sensitive Detection of Salmonella typhimurium

Jin

Wang

et al. 2023

Biosensors

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“…34,35 For example, a variety of disk-like microfluidic chips have been proposed, in which the fluid mixing and transfer are controlled by the centrifugal force. [36][37][38][39][40][41] Some other systems utilize external pumps to control the fluid in the microchips. [42][43][44][45][46][47] These devices are able to perform the extraction, purification and amplification of nucleic acids, while they still need external setups, which certainly adds inconvenience for on-site testing.…”

Section: Introductionmentioning

confidence: 99%

A point-of-care testing platform for on-site identification of genetically modified crops

Wang,

Yang,

et al. 2024

Lab Chip

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“…They can be assembled in various functional designs, and have been developed for pathogen detection [ 1 , 2 , 3 ]. Among them, the centrifugal microfluidic platform is an ideal technology that has been developed in recent years for POCT DNA detection [ 4 , 5 ]. The centrifugal microfluidic chip focuses more on the ability to integrate nucleic acid extraction and analysis [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Integrated High-Throughput Centrifugal Microfluidic Chip Device for Pathogen Detection On-Site

Lu,

Yang,

Cui

et al. 2024

Biosensors

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An integrated and high-throughput device for pathogen detection is crucial in point-of-care testing (POCT), especially for early diagnosis of infectious diseases and preventing the spread of infection. We developed an on-site testing platform that utilizes a centrifugal microfluidic chip and automated device to achieve high-throughput detection. The low-power (<32 W), portable (220 mm × 220 mm × 170 mm, 4 kg) device can complete bacterial lysis, nucleic acid extraction and purification, loop-mediated isothermal amplification (LAMP) reaction, and real-time fluorescence detection. Magnetic beads for nucleic acid adsorption can be mixed by applying electromagnetic fields and centrifugal forces, and the efficiency of nucleic acid extraction is improved by 60% compared to the no-mixing group. The automated nucleic acid extraction process achieves equivalent nucleic acid extraction efficiency in only 40% of the time consumed using the kit protocol. By designing the valve system and disc layout, the maximum speed required for the centrifugal microfluidic chip is reduced to 1500 rpm, greatly reducing the equipment power consumption and size. In detecting E. coli, our platform achieves a limit of detection (LOD) of 102 CFU/mL in 60 min. In summary, our active centrifugal microfluidic platform provides a solution for the integration of complex biological assays on turntables, with great potential in the application of point-of-care diagnosis.

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An automatic centrifugal system for rapid detection of bacteria based on immunomagnetic separation and recombinase aided amplification

Abstract: This study reported an automatic centrifugal system for rapid quantification of foodborne pathogenic bacteria based on immunomagnetic separation (IMS) for target bacteria enrichment and recombinase aided amplification (RAA) for nucleic...

Cited by 14 publications

References 36 publications

An Integrated Microfluidic Biosensing System Based on a Versatile Valve and Recombinase Polymerase Amplification for Rapid and Sensitive Detection of Salmonella typhimurium

An Integrated Microfluidic Biosensing System Based on a Versatile Valve and Recombinase Polymerase Amplification for Rapid and Sensitive Detection of Salmonella typhimurium

A point-of-care testing platform for on-site identification of genetically modified crops

Integrated High-Throughput Centrifugal Microfluidic Chip Device for Pathogen Detection On-Site

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