An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisol

Wang, Yecan; Murakami, Hiroshi; Kasama, Toshihiro; Mitsuzawa, Shigenobu; Shinkawa, Satoru; Miyake, Ryo; Takai, Madoka

doi:10.1016/j.isci.2023.107820

Cited by 2 publications

(1 citation statement)

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“…Immuno-microfluidics is a new platform for POCT that integrates immunoassay and microfluidic technologies [ 20 ]. This new technology platform has brought about breakthroughs in the biomedical field [ 21 , 22 ], integrating the basic operating units of sample processing, separation, reaction, and detection into a single chip, resulting in rapid and efficient detection with low consumption of reagents and samples [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

A Highly Sensitive Dual-Drive Microfluidic Device for Multiplexed Detection of Respiratory Virus Antigens

Yang,

Li,

Lee

et al. 2024

Micromachines

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Conventional microfluidic systems that rely on capillary force have a fixed structure and limited sensitivity, which cannot meet the demands of clinical applications. Herein, we propose a dual-drive microfluidic device for sensitive and flexible detection of multiple pathogenic microorganisms antigens/antibodies. The device comprises a portable microfluidic analyzer and a dual-drive microfluidic chip. Along with capillary force, a second active driving force is provided by a removable self-driving valve in the waste chamber. The interval between these two driving forces can be adjusted to control the reaction time in the microchannel, optimizing the formation of antigen-antibody complexes and enhancing sensitivity. Moreover, the material used in the self-driving valve can be changed to adjust the active force strength needed for different tests. The device offers quantitative analysis for respiratory syncytial virus antigen and SARS-CoV-2 antigen using a 35 μL sample, delivering results within 5 min. The detection limits of the system were 1.121 ng/mL and 0.447 ng/mL for respiratory syncytial virus recombinant fusion protein and SARS-CoV-2 recombinant nucleoprotein, respectively. Although the dual-drive microfluidic device has been used for immunoassay for respiratory syncytial virus and SARS-CoV-2 in this study, it can be easily adapted to other immunoassay applications by changing the critical reagents.

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