An E3 Ubiquitin Ligase Scaffolding Protein Is Proviral during Chikungunya Virus Infection in Aedes aegypti

Dubey, Sunil Kumar; Mehta, Divya; Chaudhary, Sakshi; Hasan, Abdul; Sunil, Sujatha

doi:10.1128/spectrum.00595-22

Cited by 9 publications

(10 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, dsRNA silencing of this gene and infection with chikungunya in an Ae. aegypti cell line results in a significant decrease in chikungunya RNA levels (Dubey et al 2022). When notch (AAEL008069), another nearby gene, is silenced, in vitro viral load of DENV-2 (dengue serotype 2) is significantly increased six days post-infection compared to control mosquitoes (Xiao et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Strong positive selection in Aedes aegypti and the rapid evolution of insecticide resistance

Love

Sikder

Vivero

et al. 2022

Preprint

View full text Add to dashboard Cite

Aedes aegypti vectors the pathogens that cause dengue, yellow fever, Zika virus, and chikungunya, and is a serious threat to public health in tropical regions. Decades of work has illuminated many aspects of Ae. aegypti's biology and global population structure, and has identified insecticide resistance genes; however, the size and repetitive nature of the Ae. aegypti genome have limited our ability to detect positive selection in this mosquito. Combining new whole genome sequences from Colombia with publicly available data from Africa and the Americas, we identify multiple strong candidate selective sweeps in Ae. aegypti, many of which overlap genes linked to or implicated in insecticide resistance. We examine the voltage-gated sodium channel gene in three American cohorts, and find evidence for successive selective sweeps in Colombia. The most recent sweep encompasses an intermediate-frequency haplotype containing four candidate insecticide resistance mutations that are in near-perfect linkage disequilibrium with one another in the Colombian sample. We hypothesize that this haplotype may continue to rapidly increase in frequency and perhaps spread geographically in the coming years. These results extend our knowledge of how insecticide resistance has evolved in this species, and add to a growing body of evidence suggesting Ae. aegypti has an extensive genomic capacity to rapidly adapt to insecticide-based vector control.

show abstract

Section: Resultsmentioning

confidence: 99%

Strong positive selection in Aedes aegypti and the rapid evolution of insecticide resistance

Love

Sikder

Vivero

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition, dsRNA silencing of this gene and infection with chikungunya in an Ae. aegypti cell line results in a significant decrease in chikungunya RNA levels ( Dubey et al 2022 ). When notch (AAEL008069), another nearby gene, is silenced, in vitro viral load of DENV-2 (dengue serotype 2) is significantly increased 6 days postinfection compared to control mosquitoes ( Xiao et al 2014 ).…”

Section: Resultsmentioning

confidence: 99%

Strong Positive Selection inAedes aegyptiand the Rapid Evolution of Insecticide Resistance

Love

Sikder

Vivero

et al. 2023

Molecular Biology and Evolution

View full text Add to dashboard Cite

Aedes aegypti vectors the pathogens that cause dengue, yellow fever, Zika virus, and chikungunya, and is a serious threat to public health in tropical regions. Decades of work has illuminated many aspects of Ae. aegypti’s biology and global population structure, and has identified insecticide resistance genes; however, the size and repetitive nature of the Ae. aegypti genome have limited our ability to detect positive selection in this mosquito. Combining new whole genome sequences from Colombia with publicly available data from Africa and the Americas, we identify multiple strong candidate selective sweeps in Ae. aegypti, many of which overlap genes linked to or implicated in insecticide resistance. We examine the voltage-gated sodium channel gene in three American cohorts, and find evidence for successive selective sweeps in Colombia. The most recent sweep encompasses an intermediate-frequency haplotype containing four candidate insecticide resistance mutations that are in near-perfect linkage disequilibrium with one another in the Colombian sample. We hypothesize that this haplotype may continue to rapidly increase in frequency and perhaps spread geographically in the coming years. These results extend our knowledge of how insecticide resistance has evolved in this species, and add to a growing body of evidence suggesting Ae. aegypti has an extensive genomic capacity to rapidly adapt to insecticide-based vector control.

show abstract

“…All of the infected samples showed a C t value of <25 and were further processed. Samples showing a RIN value >8 were used for library preparation [16]. Post-sequencing, we obtained a total of 16,177 read counts that were further analysed for differential expression.…”

Section: Transcriptional Analysis Of Aag2 Cells Reveals the Induction...mentioning

confidence: 99%

“…RNA isolation and qRT-PCR were performed per our previous protocol [16]. Briefly, RNA was isolated using the TRIzol method.…”

Section: Rna Isolation and Quantitative Real-time Pcr (Qrt -Pcr)mentioning

confidence: 99%

“…Mosquito control policies are one of the most effective strategies to control the spread of CHIKV and other arboviruses, and much effort is directed toward understanding the vector physiology to devise effective control measures [12]. Additionally, modern high-throughput platforms have been employed regularly in recent times to understand vector-pathogen interactions and to obtain a bird's eye view of the global changes that the vector undergoes when encountered by a pathogen [8,[13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oxidative stress governs mosquito innate immune signalling to reduce chikungunya virus infection in Aedes-derived cells

Mehta,

Chaudhary,

Sunil

2024

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

Arboviruses such as chikungunya, dengue and zika viruses cause debilitating diseases in humans. The principal vector species that transmits these viruses is the Aedes mosquito. Lack of substantial knowledge of the vector species hinders the advancement of strategies for controlling the spread of arboviruses. To supplement our information on mosquitoes’ responses to virus infection, we utilized Aedes aegypti-derived Aag2 cells to study changes at the transcriptional level during infection with chikungunya virus (CHIKV). We observed that genes belonging to the redox pathway were significantly differentially regulated. Upon quantifying reactive oxygen species (ROS) in the cells during viral infection, we further discovered that ROS levels are considerably higher during the early hours of infection; however, as the infection progresses, an increase in antioxidant gene expression suppresses the oxidative stress in cells. Our study also suggests that ROS is a critical regulator of viral replication in cells and inhibits intracellular and extracellular viral replication by promoting the Rel2-mediated Imd immune signalling pathway. In conclusion, our study provides evidence for a regulatory role of oxidative stress in infected Aedes-derived cells.

show abstract

An E3 Ubiquitin Ligase Scaffolding Protein Is Proviral during Chikungunya Virus Infection in Aedes aegypti

Abstract: Arboviruses cause several diseases in humans and livestock. Vector control is the main strategy for controlling diseases transmitted by mosquitoes.

Cited by 9 publications

References 83 publications

Strong positive selection in Aedes aegypti and the rapid evolution of insecticide resistance

Strong positive selection in Aedes aegypti and the rapid evolution of insecticide resistance

Strong Positive Selection inAedes aegyptiand the Rapid Evolution of Insecticide Resistance

Oxidative stress governs mosquito innate immune signalling to reduce chikungunya virus infection in Aedes-derived cells

Contact Info

Product

Resources

About