An easy way to realize SPR aptasensor: A multimode plastic optical fiber platform for cancer biomarkers detection

Cennamo, Nunzio; Pesavento, Maria; Lunelli, Lorenzo; Vanzetti, Lia; Pederzolli, Cecilia; Zeni, Luigi; Pasquardini, Laura

doi:10.1016/j.talanta.2015.03.025

Cited by 105 publications

(67 citation statements)

References 37 publications

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“…Another SPR-based biosensor is reported that can detect VEGF in a nanomolar range. Here DNA aptamers are used as the biorecognition molecules (Cennamo et al, 2015). An LSPR based sensor is developed by Zhao et al (2014) for the detection of a cervical cancer marker, squamous cell carcinoma antigen (SCCa) using an AgNPs array where monoclonal anti-SCCa antibodies are immobilized.…”

Section: Spr Based Detection Techniquesmentioning

confidence: 99%

Recent advances in biosensor development for the detection of cancer biomarkers

Jayanthi

Das

Saxena

2017

Biosensors and Bioelectronics

421

169

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Section: Spr Based Detection Techniquesmentioning

confidence: 99%

Recent advances in biosensor development for the detection of cancer biomarkers

Jayanthi

Das

Saxena

2017

Biosensors and Bioelectronics

421

169

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“…The sensing platform presented here uses a side‐polished multimode plastic optical fibre (POF) as the light guiding structure, which has particular advantages due to its low cost, easy manipulation, excellent flexibility, great numerical aperture and large diameter, along with the fact that plastic is able to withstand smaller bend radii than glass . Gold is chosen as the plasmonic responsible layer in the sensor platform because of the extremely well understood chemical interaction with organic compounds and its good resistance to oxidation and corrosion in different environments . In addition, the gold‐coated in‐POF sensor is integrated into a new thermo‐stabilized microfluidic platform.…”

Section: Introductionmentioning

confidence: 99%

SPR-based plastic optical fibre biosensor for the detection of C-reactive protein in serum

et al. 2016

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A plastic optical fibre biosensor based on surface plasmon resonance for the detection of C-reactive protein (CRP) in serum is proposed. The biosensor was integrated into a home-made thermo-stabilized microfluidic system that allows avoiding any thermal and/or mechanical fluctuation and maintaining the best stable conditions during the measurements. A working range of 0.006-70 mg L and a limit of detection of 0.009 mg L were achieved. These results are among the best compared to other SPR-based biosensors for CRP detection, especially considering that they were achieved in a real and complex medium, i.e. serum. In addition, since the sensor performances satisfy those requested in physiologically-relevant clinical applications, the whole biosensing platform could well address high sensitive, easy to realize, real-time, label-free, portable and low cost diagnosis of CRP for future lab-on-a-chip applications. 3D sketch (left) of the thermo-stabilized home-made flow cell developed to house the SPR-based plastic optical fibre biosensor. Exemplary response curve (shift of the SPR wavelength versus time) of the proposed biosensor (right) for the detection of C-reactive protein in serum.

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“…The transmitted power increased linearly when the sensor is exposed to sucrose solutions of increasing refractive index, although a complex procedure and expensive instrumentation are required for the sensor production. Cennamo et al have reported several biological and chemical sensors based on SPR (surface plasmon resonance) in a D-shaped POF platform [11,12,13] with 1 cm length sensing region and with typical optical resolution of around 6 × 10 −4 RIU [14]. …”

Section: Introductionmentioning

confidence: 99%

Refractive Index Sensing with D-Shaped Plastic Optical Fibers for Chemical and Biochemical Applications

Sequeira

Duarte

Bilro

et al. 2016

Sensors

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We report the optimization of the length of a D-shaped plastic optical fiber (POF) sensor for refractive index (RI) sensing from a numerical and experimental point of view. The sensing principle is based on total internal reflection (TIR). POFs with 1 mm in diameter were embedded in grooves, realized in planar supports with different lengths, and polished to remove the cladding and part of the core. All D-shaped POF sensors were tested using aqueous medium with different refractive indices (from 1.332 to 1.471) through intensity-based configuration. Results showed two different responses. Considering the refractive index (RI) range (1.33–1.39), the sensitivity and the resolution of the sensor were strongly dependent on the sensing region length. The highest sensitivity (resolution of 6.48 × 10−3 refractive index units, RIU) was obtained with 6 cm sensing length. In the RI range (1.41–1.47), the length of the sensing region was not a critical aspect to obtain the best resolution. These results enable the application of this optical platform for chemical and biochemical evanescent field sensing. The sensor production procedure is very simple, fast, and low-cost.

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An easy way to realize SPR aptasensor: A multimode plastic optical fiber platform for cancer biomarkers detection

Cited by 105 publications

References 37 publications

Recent advances in biosensor development for the detection of cancer biomarkers

Recent advances in biosensor development for the detection of cancer biomarkers

SPR-based plastic optical fibre biosensor for the detection of C-reactive protein in serum

Refractive Index Sensing with D-Shaped Plastic Optical Fibers for Chemical and Biochemical Applications

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