An eco-friendly modified methodology for the resolution of binary pharmaceutical mixtures based on self-deconvolution of the UV spectrophotometric spectra in the Fourier domain: Application of Fourier self-deconvolution in UV spectroscopy

Elsonbaty, Ahmed; Attala, Khaled

doi:10.1016/j.saa.2021.120262

Cited by 21 publications

(11 citation statements)

References 13 publications

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“…2 ), and XIP was resolved and quantified by FSD method at 261.0 nm. The FSD approach tends to reduce excessively overlapping spectra, enhancing the resolution of overlapping spectra by eliminating the broadening impact caused by the spectrophotometer convolution [ 17 ].…”

Section: Resultsmentioning

confidence: 99%

“…The physical convolution brought on by instrumental distortions to spectrum signals was eliminated by the currently employed technique. What gives the current methodology its resolving capacity is the evolution of major spectral zones with zero-crossing or no contribution points between the spectra of mixed components [ 17 ]. Reversing the physical convoluting, distorting impacts from the spectrophotometer performed to the recorded spectrum is the underlying idea behind FSD [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

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Univariate versus multivariate spectrophotometric data analysis of triamterene and xipamide; a quantitative and qualitative greenly profiled comparative study

et al. 2023

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Triamterene (TRI) and xipamide (XIP) mixture is used as a binary medication of antihypertension which is considered as a major cause of premature death worldwide. The purpose of this research is the quantitative and qualitative analysis of this binary mixture by green univariate and multivariate spectrophotometric methods. Univariate methods were zero order absorption spectra method (D0) and Fourier self-deconvolution (FSD), as TRI was directly determined by D0 at 367.0 nm in the range (2.00–10.00 µg/mL), where XIP show no interference. While XIP was determined by FSD at 261.0 nm in the range (2.00–8.00 µg/mL), where TRI show zero crossing. Multivariate methods were Partial Least Squares, Principal Component Regression, Artificial Neural Networks, and Multivariate Curve Resolution-Alternating Least Squares. A training set of 25 mixtures with different quantities of the tested components was used to construct and evaluate them, 3 latent variables were displayed using an experimental design. A set of 18 synthetic mixtures with concentrations ranging from (3.00–7.00 µg/mL) for TRI and (2.00–6.00 µg/mL) for XIP, were used to construct the calibration models. A collection of seven synthetic mixtures with various quantities was applied to build the validation models. All the proposed approaches quantitative analyses were evaluated using recoveries as a percentage, root mean square error of prediction, and standard error of prediction. Strong multivariate statistical tools were presented by these models, and they were used to analyze the combined dosage form available on the Egyptian market. The proposed techniques were evaluated in accordance with ICH recommendations, where they are capable of overcoming challenges including spectral overlaps and collinearity. When the suggested approaches and the published one were statistically compared, there was no discernible difference between them. The green analytical method index and eco-scale tools were applied for assessment of the established models greenness. The suggested techniques can be used in product testing laboratories for standard pharmaceutical analysis of the substances being studied.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Univariate versus multivariate spectrophotometric data analysis of triamterene and xipamide; a quantitative and qualitative greenly profiled comparative study

et al. 2023

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“…Accuracy was studied by performing recovery experiments. [42][43][44][45][46] The accuracy of the proposed HPLC method was established at 80%, 100% and 120% level. The placebo was added to the standard solution and % recovery was calculated.…”

Section: Accuracymentioning

confidence: 99%

“…The precision of the proposed HPLC method was studied by performing repeatability study and intraday precision for both the drugs. [43][44][45][46] The repeatability study was performed by injecting 40 mcg/ml of Celecoxib and 4 mcg/ml for Amlodipine (n=6). The intraday precision was studied by injecting 20, 30 and 40 mcg/ml for Celecoxib and 2, 3 and 4 µg/mL for Amlodipine (n=3) and % RSD was calculated.…”

Section: Precisionmentioning

confidence: 99%

“…Limit of detection (LOD) and Limit of quatification (LOQ) were determined for Celecoxib and Amlodipine according to ICH guidelines Q2 (R1) 41,[44][45][46] . LOD is the smallest concentration of analyte which gives measurable response (signal to noise ratio for LOD is 3.3) while LOQ is the smallest concentration of analyte which can be quantified.…”

Section: Limit Of Detection and Limit Of Quantificationmentioning

confidence: 99%

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Response Surface Methodology Based Development and Quantification of Celecoxib and Amlodipine Using RP-HPLC.

Shah

Patel

Shah

2022

Int J Life Sci Pharm Res

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A novel RP-HPLC method for estimation of Amlodipine Besylate and Celecoxib using an experimental design approach employing response surface technique was developed and validated using a C18 column and its application in marketed formulation. A multivariate optimization of experimental conditions was carried out using experimental design employing organic content in the mobile phase, pH, and flow rate of the mobile phase as three independent variables. The aim of the study was to apply response surface methodology and to study the effect of the independent variables on separation and estimation of both drugs by RP-HPLC method using faced central composite (FCC) experimental design which is novel in this area of research. Optimization of retention time of the last eluting peak and peak symmetry was performed using Derringer’s desirability function in which potassium dihydrogen phosphate buffer with pH 4. 45, 25 mM, 0.5% Triethylamine and acetonitrile in an isocratic proportion of 70:30% v/v and 1.2 ml/min flow rate was found to be the predicted optimum condition. DoE approach based RP-HPLC method development using response surface methodology led to efficient separation with a lower retention time of eluted peaks below 7 min indicating novelty of research. A linear response was observed over the concentration range of 40-240 μg/mL for Celecoxib and 1-6 μg/ mL for Amlodipine Besylate. Limit of detection (LOD) and limit of quantitation (LOQ) for Celecoxib were found to be 0.02 μg/mL and 0.08μg/mL, and for Amlodipine Besylate were 0.0053 μg/mL and 0.0162 μg/mL, respectively. The method was successfully validated in accordance with ICH guideline acceptance criteria for linearity, accuracy, precision, specificity, robustness.

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Greenness‐by‐design approach for developing novel UV spectrophotometric methodologies resolving a quaternary overlapping mixture

Abdelshakour

Eissa

Attala

et al. 2023

Archiv der Pharmazie

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Greenness-by-design (GbD) is an approach that integrates green chemistry principles into the method development stage of analytical processes, aiming to reduce their environmental impact. In this work, we applied GbD to a novel univariate double divisor corrected amplitude (DDCA) method that can resolve a quaternary pharmaceutical mixture in a fixed-dose polypill product. We also used a genetic algorithm as a chemometric modeling technique to select the informative variables for the analysis of the overlapping mixture. This resulted in more accurate and efficient predictive models. We used a computational approach to study the effect of solvents on the spectral resolution of the mixture and to minimize the spectral interferences caused by the solvent, thus achieving spectral resolution with minimal analytical effort and ecological footprint. The validated methods showed wide linear concentration ranges for the four components (1-30 µg/mL for losartan, 2.5-30 µg/mL for atorvastatin and aspirin, and 2.5-35 µg/mL for atenolol) and achieved high scores on the hexagon and spider charts, demonstrating their eco-friendliness.

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An eco-friendly modified methodology for the resolution of binary pharmaceutical mixtures based on self-deconvolution of the UV spectrophotometric spectra in the Fourier domain: Application of Fourier self-deconvolution in UV spectroscopy

Cited by 21 publications

References 13 publications

Univariate versus multivariate spectrophotometric data analysis of triamterene and xipamide; a quantitative and qualitative greenly profiled comparative study

Univariate versus multivariate spectrophotometric data analysis of triamterene and xipamide; a quantitative and qualitative greenly profiled comparative study

Response Surface Methodology Based Development and Quantification of Celecoxib and Amlodipine Using RP-HPLC.

Greenness‐by‐design approach for developing novel UV spectrophotometric methodologies resolving a quaternary overlapping mixture

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