An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular docking

Chelliah, Ramachandran; Saravanakumar, Kandasamy; Daliri, Eric Banan‐Mwine; Kim, Joong‐Hark; Lee, Jung‐Kun; Jo, Hyeon-Yeong; Madar, Inamul Hasan; Kim, Se‐Hun; Ramakrishnan, S.; Oh, Deog‐Hwan; Barathikannan, Kaliyan; Ofosu, Fred Kwame; Hwang, Su-Bin; Park, Eun-Ji; Elahi, Fazle; Wang, Myeong‐Hyeon; Oh, Deog‐Hwan

doi:10.1016/j.dib.2020.105745

Cited by 3 publications

(5 citation statements)

References 10 publications

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“…Further, the minimum inhibitory concentration was determined based on the growth curve method [ 31 ]. Further, the minimum inhibitory concentration (MIC) of different plant extracts was determined based on growth inhibitory assay [ 33 , 34 ] against indicator pathogens such as E. coli , S. aureus , S. typi , S. dysenteriae, V. cholera and S. flexneri ( Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

In Vitro Probiotic Evaluation of Saccharomyces boulardii with Antimicrobial Spectrum in a Caenorhabditis elegans Model

Chelliah

Kim

Daliri

et al. 2021

Foods

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In the present study, we screened for potential probiotic yeast that could survive under extreme frozen conditions. The antimicrobial and heat-stable properties of the isolated yeast strains Saccharomyces boulardii (S. boulardii) (KT000032, KT000033, KT000034, KT000035, KT000036, and KT000037) was analyzed and compared with commercial probiotic strains. The results revealed that the tested S. boulardii KT000032 strain showed higher resistance to gastric enzymes (bile salts, pepsin, and pancreatic enzyme) at low pH, with broad antibiotic resistance. In addition, the strain also showed efficient auto-aggregation and co-aggregation abilities and efficient hydrophobicity in the in-vitro and in-vivo C. elegens gut model. Further, the KT000032 strain showed higher antimicrobial efficiency against 13 different enteropathogens and exhibited commensal relationships with five commercial probiotic strains. Besides, the bioactive compounds produced in the cell-free supernatant of probiotic yeast showed thermo-tolerance (95 °C for two hours). Furthermore, the thermo-stable property of the strains will facilitate their incorporation into ready-to-eat food products under extreme food processing conditions.

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Section: Resultsmentioning

confidence: 99%

In Vitro Probiotic Evaluation of Saccharomyces boulardii with Antimicrobial Spectrum in a Caenorhabditis elegans Model

Chelliah

Kim

Daliri

et al. 2021

Foods

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“…The spread plate method was used to inoculate each microorganism on nutrient agar plates. The plates were incubated at 37 °C for 24 h, and the diameter of the zone of inhibition was measured and compared to evaluate the antibacterial potential of the different extracts [ 51 ].…”

Section: Methodsmentioning

confidence: 99%

“…The growth kinetics studies were conducted by adding different concentrations of protein to pre-diluted overnight cultures (0.1 OD) to a final volume of 1 mL in a sterile cuvette and incubated at 37 °C for 4–6 h with continuous shaking. The OD at 600 nm was measured every 60 min by spectrophotometer (Eppendorf) and values were recorded [ 51 ].…”

Section: Methodsmentioning

confidence: 99%

“…Bacterial cells were set at 30 ± 2 °C for 1 h with 2% glutaraldehyde and paraformaldehyde. After many washes with 0.1 M cacodylate buffer, cells were dehydrated with ethanol [ 51 ]. Then, bacterial cells were injected with increasingly concentrated Eponate 812 and then polymerized at 60 ± 2 °C for 2 days.…”

Section: Methodsmentioning

confidence: 99%

“…Sequential identification of peptides by LC-ESI-TOF-MS/MS (mass spectrometry liquid chromatography-electrospray ionization-quantitative time-of-flight tandem mass spectrometry) were analyzed at the National Instrumentation Center for Environmental Management of Seoul National University in Seoul, Korea, according to an earlier method by Chelliah et al [ 51 ]. The peptides were inspected using QS 3.0 software (Applied Biosystems, Seoul, Korea).…”

Section: Methodsmentioning

confidence: 99%

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In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in Brassica juncea and Moringa oleifera

et al. 2021

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The study aimed to investigate the antibacterial activity of Mustard (Brassica juncea) and Moringa (Moringa oleifera) leaf extracts and coagulant protein for their potential application in water treatment. Bacterial cell aggregation and growth kinetics studies were employed for thirteen bacterial strains with different concentrations of leaf extracts and coagulant protein. Moringa oleifera leaf extract (MOS) and coagulant protein showed cell aggregation against ten bacterial strains, whereas leaf extract alone showed growth inhibition of five bacterial strains for up to 6 h and five bacterial strains for up to 3 h. Brassica juncea leaf extract (BJS) showed growth inhibition for up to 6 h, and three bacterial strains showed inhibition for up to 3 h. The highest inhibition concentration with 2.5 mg/mL was 19 mm, and furthermore, the minimum inhibitory concentration (MIC) (0.5 mg/mL) and MBC (1.5 mg/mL) were determined to have a higher antibacterial effect for <3 KDa peptides. Based on LCMS analysis, napin was identified in both MOS and BJS; furthermore, the mode of action of napin peptide was determined on lipoprotein X complex (LpxC) and four-chained structured binding protein of bacterial type II topoisomerase (4PLB). The docking analysis has exhibited moderate to potent inhibition with a range of dock score −912.9 Kcal/mol. Thus, it possesses antibacterial-coagulant potential bioactive peptides present in the Moringa oleifera purified protein (MOP) and Brassica juncea purified protein (BJP) that could act as an effective antimicrobial agent to replace currently available antibiotics. The result implies that MOP and Brassica juncea purified coagulant (BJP) proteins may perform a wide degree of antibacterial functions against different pathogens.

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Review and perspective on bioactive peptides: A roadmap for research, development, and future opportunities

2022

Journal of Agriculture and Food Research

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An effective datasets describing antimicrobial peptide produced from Pediococcus acidilactici - purification and mode of action determined by molecular docking

Cited by 3 publications

References 10 publications

In Vitro Probiotic Evaluation of Saccharomyces boulardii with Antimicrobial Spectrum in a Caenorhabditis elegans Model

In Vitro Probiotic Evaluation of Saccharomyces boulardii with Antimicrobial Spectrum in a Caenorhabditis elegans Model

In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in Brassica juncea and Moringa oleifera

Review and perspective on bioactive peptides: A roadmap for research, development, and future opportunities

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