An effective method for harvesting of marine microalgae: pH induced flocculation

Pérez, Leticia; Salgueiro, Jose Luis; Maceiras, R.; Cancela, Ángeles; Sánchez, Ángel

doi:10.1016/j.biombioe.2016.12.010

Cited by 58 publications

(21 citation statements)

References 14 publications

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“…Three samples were collected daily from each culture and all measurements were carried out in triplicate. Additionally, at the end of each experimental period, the total production of each culture was measured (in g per 50 L), after harvest of the biomass by a process which was aided by raising the pH of the culture medium, in order to cause the algae to flocculate . The pH was raised by adding sodium hydroxide, a base, which induces more than 90% flocculation at pH 11, according to Safi et al .…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, at the end of each experimental period, the total production of each culture was measured (in g per 50 L), after harvest of the biomass by a process which was aided by raising the pH of the culture medium, in order to cause the algae to flocculate. 17,18 The pH was raised by adding sodium hydroxide, a base, which induces more than 90% flocculation at pH 11, according to Safi et al 19 After sedimentation, the supernatant medium was removed, the condensates were collected and their excess water was evaporated in an air circulation oven at 40 ∘ C until dry. Biomass (7-10%) was added in the final measurements in order to determine the total production in each bioreactor.…”

Section: Microalgal Biomass Concentrationmentioning

confidence: 99%

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Effect of nitrogen concentration, two‐stage and prolonged cultivation on growth rate, lipid and protein content of Chlorella vulgaris

Metsoviti

Katsoulas

Karapanagiotidis

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND The effects of the initial nitrogen concentration, as well as of two‐stage and prolonged cultivation on the growth rate, lipid and protein content of Chlorella vulgaris were examined. Additionally, a kinetic model of specific growth rate in the exponential growth phase (µexp) was developed as a function of initial nitrogen concentration. RESULTS As the initial nitrogen concentration in the culture medium increased, the total biomass production and µexp went through a maximum at 300 mg N L−1 and at 200 mg N L−1, respectively. The lipid content was found to be highly dependent on the initial nitrogen concentration and increased from 9.0% to 29.6% as the nitrogen concentration varied from 800 to 10 mg N L−1, respectively. Cultivation in two stages resulted in an increase of the lipid content of the biomass from 15.4% to 24.9%, while prolonged cultivation led to an increase of the lipid content from 15.4% to 23.0%. CONCLUSION Cultivation under optimum nitrogen concentration can lead to increased biomass production. In addition, optimization of lipid production can be carried out by a two‐stage cultivation mode or by prolonged cultivation. © 2018 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Section: Microalgal Biomass Concentrationmentioning

confidence: 99%

Effect of nitrogen concentration, two‐stage and prolonged cultivation on growth rate, lipid and protein content of Chlorella vulgaris

Metsoviti

Katsoulas

Karapanagiotidis

et al. 2019

J of Chemical Tech & Biotech

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show abstract

“…At the end of each experiment, the total productivity of each culture was measured (in g L −1 d −1 ) as dry weight (dw), after harvest of the biomass by a process which was aided by raising the pH of the culture medium, in order to cause the microalgae to flocculate [31,32]. The pH was raised by adding sodium hydroxide, which induces more than 90% flocculation at pH 11 [33].…”

Section: Measurementsmentioning

confidence: 99%

Effect of Light Intensity and Quality on Growth Rate and Composition of Chlorella vulgaris

Metsoviti

Papapolymerou

Karapanagiotidis

et al. 2019

Plants

161

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In this research, the effect of solar irradiance on Chlorella vulgaris cultivated in open bioreactors under greenhouse conditions was investigated, as well as of ratio of light intensity in the 420–520 nm range to light in the 580–680 nm range (I420–520/I580–680) and of artificial irradiation provided by red and white LED lamps in a closed flat plate laboratory bioreactor on the growth rate and composition. The increase in solar irradiance led to faster growth rates (μexp) of C. vulgaris under both environmental conditions studied in the greenhouse (in June up to 0.33 d−1 and in September up to 0.29 d−1) and higher lipid content in microalgal biomass (in June up to 25.6% and in September up to 24.7%). In the experiments conducted in the closed bioreactor, as the ratio I420–520/I580–680 increased, the specific growth rate and the biomass, protein and lipid productivities increased as well. Additionally, the increase in light intensity with red and white LED lamps resulted in faster growth rates (the μexp increased up to 0.36 d−1) and higher lipid content (up to 22.2%), while the protein, fiber, ash and moisture content remained relatively constant. Overall, the trend in biomass, lipid, and protein productivities as a function of light intensity was similar in the two systems (greenhouse and bioreactor).

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“…In their study, effect of acidic pH values and basic pH values on both species were investigated. It was found that, basic pH values were more effective than acidic pH and the highest biomass recovery was achieved at pH 11–12 . Vandamme et al explored harvesting of Chlorella vulgaris via pH induced flocculation.…”

Section: Resultsmentioning

confidence: 99%

Optimization of pH induced flocculation of marine and freshwater microalgae via central composite design

Akış

Özçimen

2019

Biotechnology Progress

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Microalgae harvesting via pH induced flocculation along with utilization of recovered medium after flocculation is one of the most economical methods for separating the microalgal biomass in order to reduce the dewatering cost. In this study, optimization of marine and freshwater microalgae flocculation by pH adjustment was investigated via central composite design methodology. One molar of KOH and NaOH solutions were used to increase the pH level of the microalgal culture. Increasing pH value of the medium provided the highest flocculation efficiency up to 92.63 and 86.18% with pH adjusted to 10.5 with KOH and NaOH solutions for marine microalgae Nannochloropsis oculata and freshwater microalgae Chlorella minutissima, respectively. Also, it was revealed that microalgae cells were still alive after flocculation process and their biochemical composition was not changed, and flocculated medium can be used again for the next microalgal production. According to the results, it can be said that this method is cheap and effective, simple to operate and provides the utilization of flocculated medium again.

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An effective method for harvesting of marine microalgae: pH induced flocculation

Cited by 58 publications

References 14 publications

Effect of nitrogen concentration, two‐stage and prolonged cultivation on growth rate, lipid and protein content of Chlorella vulgaris

Effect of nitrogen concentration, two‐stage and prolonged cultivation on growth rate, lipid and protein content of Chlorella vulgaris

Effect of Light Intensity and Quality on Growth Rate and Composition of Chlorella vulgaris

Optimization of pH induced flocculation of marine and freshwater microalgae via central composite design

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