An Effective Way to Carry Out Mass in Vitro Propagation of Potentilla Alba L.

Abstract

Despite the plant's extensive area of distribution, Potentilla alba L. natural resources are scarce and cannot meet the modern needs of the pharmaceutical industry. Because of the mass preparation of medical raw materials by using P. alba, it entered into the list of rare and endangered species plants of the Red Data Book of the Republic of Belarus. This plant is not represented in the wild flora of Western Siberia, but there is a great need for developing a method for the mass propagation of P. alba using in vitro culture in order to obtain a high-quality planting material. At the explant stage, the technique of the P. alba introduction into in vitro culture is developed. This paper reveals the morphogenetic features of the development of P. alba explants of different types and the regenerative capacity of the tissue culture. At the micropropagation stage, the optimum culture media and the growth conditions for the regenerated plants are selected. At the stage of test-tube plants rooting and transferring them into ex vitro conditions, the most effective means of adaptation to non-sterile conditions in hydroponics are proposed.

Microclonal propagation of Dasiphora fruticosa (Rosaceae)

“…Our study with D. fruticosa also revealed high propagation co efficient for the representative of the Rosaceae family with different growth form -a shrub. Herbaceous plants are often characterized with a high propagation coefficient, but for P. alba grown on a hormone-free medium it was equal to 1 (Tikhomirova et al 2016). It is likely, that different species of Rosaceae family have different breeding potential.…”

“…Studies on vegetative propagation of the Kuril tea were conducted previously, including propagation for industrial production (Korzun & Vavilova 2015, Urusov & Lobanova 2018. Some species of Rosaceae family such as Potentilla alba and intergeneric hybrids (Fragaria vesca × Potentilla fruticosa, Fragaria × Potentilla palustris) were propagated microclonally in vitro (Silva & Jones 1996, Sutan et al 2010, Tikhomirova et al 2016. Meanwhile, microclonal propagation me thod for valuable specimens of uterine plants offers great op por tunities for mass plant propagation, due to a high bree ding rate (up to 10 5 -10 6 specimens per year from 1 plant), possibility of all year round cultivation, little space for sterile microplant cultivation, elimination of plants from vi ruses, and long-term storage of microplants in tubes at low temperatures, allowing to establish a bank of genotypes of valuable species and forms (Kataeva & Butenko 1983).…”

New Perspectives for the Use of Potentilla alba Rhizomes to Treat Thyroid Gland Impairments

“…P. alba was also mentioned in the herbal book Tabernaemontanus dating from 1588, with similar indications for use [17]. Since the 18th century, in Eastern Europe and particularly in Belarusian Polesie, there has been a common practice of using P. alba decoctions as a substitute for Camellia sinensis [18].…”

Features of the Root and Rhizome Anatomical Structure of Potentilla Alba L. As a Diagnostic Sign of the Raw Materials

“…Using this method, the area of P. alba plantations can be quickly and effectively increased. Therefore, Tikhomirova and others developed a method of microclonal reproduction of P. alba [4]. However, the most common method of obtaining medicinal raw materials is the gathering of wild plants, which has a debilitating effect on natural biodiversity.…”