An efficient and comprehensive plant glycerolipids analysis approach based on high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometer

Abstract

In past two decades, numerous lipidomics approaches based on mass spectrometry with or without liquid chromatography separation have been established for identification and quantification of lipids in plants. In this study, we developed an efficient and comprehensive lipidomics approach based on UPLC with an Acquity UPLCTM BEH C18 column coupled to TripleTOF using ESI in positive ion mode and MS/MSALL scan for data collection. Lipid extract was prepared to 2 mg/ml solution according to dry tissue weight and mixed with 13 kinds of internal standards including PA, PC, PE, and PG. Each analysis required single injection of 5–10 μl lipid solvent and completed in 32 min. A target method dataset was generated using the LipidView software for prediction of the accurate mass of target lipid species. The dataset was uploaded into the PeakView to create processing datasets to search target lipid species, which achieved batch data processing of multiple samples for lipid species‐specific identification and quantification. As proof of concept, we profiled the lipids of different tissues of rapeseed. Thirteen lipid classes including 218 glycerolipids were identified including 46 TAGs, 15 DAGs, 20 PCs, 24 PEs, 13 PGs, 14 PIs, 26 PSs, 12 PAs, 16 MGDGs, 16 DGDGs, 6 LysoPCs, 5 LysoPEs, and 5 LysoPGs. Together, our approach permits the analysis of glycerolipids in plant tissues with simplicity in sample analysis and data processing using UPLC‐TripleTOF.

Genome- and transcriptome-wide association studies provide insights into the genetic basis of natural variation of seed oil content in Brassica napus

“…Seed oil biosynthesis starts approximately 20 days after flowering (DAF), and oil accumulates actively at 40 DAF in B. napus (Lu et al, 2019b). Thus, we sequenced seed transcriptomes at two developmental stages (309 accessions at 20 DAF and 274 accessions at 40 DAF; Supplemental Table 4).…”

Lipidomic and transcriptomic analysis reveals reallocation of carbon flux from cuticular wax into plastid membrane lipids in a glossy “Newhall” navel orange mutant

“…Accurate molecular weight and fragmentation ion information were acquired using LipidView 2.0 to generate an in-house dataset. The identification of glycerolipid classes and species was based on retention time, accurate m/z, and fragmentation ion patterns according to a previous report 50 .…”

Root Involvement in Plant Responses to Adverse Environmental Conditions

“…Lipidomics analyzes membrane lipid composition and has been used to decipher the role of lipids on tolerance to abiotic stress conditions [56]. Electrospray ionization tandem mass spectrometry (ESI-MS/MS) has been traditionally used for lipidomics although other mass spectrometry instruments such as HILIC-ESI-IT-TOF-MS/MS or UPLC-TripleTOF make lipid analyses more efficient and accurate [57]. Although this is a promising approach to study plant stress, its use in roots is limited.…”