An Efficient Bioemulsifier-Producing Bacillus subtilis UCP 0146 Isolated from Mangrove Sediments

Maia, Patrícia C. V. S.; Santos, Vanessa P.; Fereira, Adriana S.; Luna, Marcos Antônio Cavalcanti; Silva, Thayse A. L.; Andrade, Rosileide F. S.; Campos‐Takaki, Galba Maria de

doi:10.3390/colloids2040058

Cited by 13 publications

(22 citation statements)

References 42 publications

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“…The result obtained in relation to the yield of isolated biomolecule corroborates the data found in the literature. These describe yields for bioemulsifiers of microbiological origin between 0.1 and 3.15 g/L [25,30,[37][38][39]. The yield of the fungal bioemulsifier of this study was superior to the bacterial of Lactobacillus plantarum, obtaining only 0.7 g/L of the isolate [39].…”

Section: Discussionmentioning

confidence: 99%

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Eco-Friendly Bioemulsifier Production by Mucor circinelloides UCP0001 Isolated from Mangrove Sediments Using Renewable Substrates for Environmental Applications

et al. 2020

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The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. In the present work, an economically attractive medium composed of corn steep liquor and waste cooking oil was formulated to maximize the production of bioemulsifier by Mucor circinelloides UCP0001. A central rotational composite design was applied to statistical validation of the production. The emulsifying properties, stability under extreme conditions, its toxicity character, and the characterization of the bioemulsifier were determined. The best condition for biomolecule synthesis occurred in the assay 2 containing 4% of corn steep liquor and 3% waste soybean oil and exhibited 100% emulsification index for canola oil and petroleum, as well as excellent emulsifying activity for canola oil and burned engine oil. The nutritional factors studied showed statistical relevance, since all linear, quadratic effects and their interactions were significant. The bioemulsifier showed 2.69 g/L yield and the chemical character of the molecule structure was identified by FT-IR (Fourier Transform Infrared) spectroscopy. The bioemulsifier showed no toxicity to Artemia salina and Chlorella vulgaris. Stable emulsions were obtained under extreme conditions of temperature, pH, and salinity. These findings contribute to understanding of the relationship between production, physical properties, chemical composition, and stability of bioemulsifier for their potential applications in biotechnology, such as bioremediation of hydrocarbon-contaminated soil and water.

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Section: Discussionmentioning

confidence: 99%

“…[19]. A recent study selected several cultivation aspects to investigate bioemulsifier production by Bacillus subtilis grown in cassava wastewater, and found that only inoculum size was statistically significant in biomolecule production [25].…”

Section: Discussionmentioning

confidence: 99%

Eco-Friendly Bioemulsifier Production by Mucor circinelloides UCP0001 Isolated from Mangrove Sediments Using Renewable Substrates for Environmental Applications

et al. 2020

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“…Cell-free metabolic liquid was submitted separately at different temperatures (0, 5, 30, 37, 50, and 100°C), adjusted to different pH values (2, 4, 6, 8, 10, and 12) or NaCl (2, 4, 6, 8, 10, and 12%). en, EI 24 was determined using burned motor oil as a hydrophobic substrate following the methodology previously described in Section 2.4.4 [25,26].…”

Section: Determination Of the Stability Of The Bioemulsifiermentioning

confidence: 99%

“…e effect of the bioemulsifier produced by S. maltophilia UCP 1601 on the viscosity of hydrophobic compounds (WSO and burned motor oil) was investigated using the methodology described by Andrade Silva et al [27] and Maia et al [26]. e viscosity of the hydrophobic compounds was determined in test tubes before and after adding 2 ml of the cell-free metabolic liquid (crude bioemulsifier) of the selected condition of the FFD, and the mixture was vigorously homogenized in vortex for 1 min.…”

Section: Effect Of the Bioemulsifier On The Viscosity Of Hydrophobicmentioning

confidence: 99%

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Bioconversion of Agroindustrial Waste in the Production of Bioemulsifier by Stenotrophomonas maltophilia UCP 1601 and Application in Bioremediation Process

Nogueira

Rodríguez

Andradade

et al. 2020

International Journal of Chemical Engineering

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This study investigated the potential of the bacterium Stenotrophomonas maltophilia UCP 1601 to produce a new biomolecule with emulsifying properties by determining the hemolytic activity, obtaining a halo of 9 mm in blood agar. Fermentations were carried out in saline mineral medium supplemented with 10% waste soybean oil (WSO) and different concentrations of glucose, peptone, ZnCl2, and MgSO4, according to a 24 full-factorial design. The results showed that the best results were obtained in condition 6 (medium composed of 4% glucose, 1% peptone, 2.72% ZnCl2, and 2.46% MgSO4), with excellent high emulsification index of 82.74%, using burned motor oil. The emulsifying property of the biomolecule produced was confirmed by the emulsification index of 78.57, 54.07, and 58.62%, using soybean, corn, and diesel oils, respectively, and the stability at different values of pH, temperature, and NaCl concentrations. The yield of the produced bioemulsifier was 2.8 g/L, presenting an anionic character and polymeric nature (37.6% lipids, 28.2% proteins, and 14.7% carbohydrates), confirmed by FTIR. The new bioemulsifier demonstrated promising potential for bioremediation of hydrophobic contaminants in the environment, since it had the ability to reduce the viscosity of WSO and burned motor oil, as well as excellent dispersion capacity of the burned motor oil in water (69.94 cm2 of oil displacement area), and removing 71.7% of this petroleum derivative from sandy soil.

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Production of surfactin by Bacillus subtilis LAMI005 and evaluation of its potential as tensoactive and emulsifier

et al. 2021

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In this study, the kinetic of lipopeptide biosurfactant production by Bacillus subtilis LAMI005 and its tensoactive and emulsifier properties were evaluated. The influence of the concentrations of nitrogen ((NH4)2SO4) and carbon source (glucose and fructose) on biosurfactant production, using a mineral medium, were evaluated. A production of about 930 mg · L−1 of surfactin was observed for mineral medium supplied with total reducing sugars (16.55 g · L−1) and (NH4)2SO4 (1 g · L−1) as carbon and nitrogen sources, respectively. The crude biosurfactant exhibited its potential as tensoactive, presenting the capability to reach surface tension of about 28 mN ⋅ m−1 for water and exhibiting low CMC (~14–20 mg · L−1). The analysis of emulsifying activity showed a greater stability of emulsion formed in motor oil (3.5 U), soybean oil (2.0 U), and biodiesel (1.5 U). The emulsion formulated on motor oil remained stable for more than 200 h, which was explained by its average drop diameter (~0–10 μm). The surface‐active properties of surfactin did not seem to be affected by different NaCl concentrations, which may promote its applicability in high salt environments, such as oil spills in marine areas.

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An Efficient Bioemulsifier-Producing Bacillus subtilis UCP 0146 Isolated from Mangrove Sediments

Cited by 13 publications

References 42 publications

Eco-Friendly Bioemulsifier Production by Mucor circinelloides UCP0001 Isolated from Mangrove Sediments Using Renewable Substrates for Environmental Applications

Eco-Friendly Bioemulsifier Production by Mucor circinelloides UCP0001 Isolated from Mangrove Sediments Using Renewable Substrates for Environmental Applications

Bioconversion of Agroindustrial Waste in the Production of Bioemulsifier by Stenotrophomonas maltophilia UCP 1601 and Application in Bioremediation Process

Production of surfactin by Bacillus subtilis LAMI005 and evaluation of its potential as tensoactive and emulsifier

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