An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.)

Abstract

AbstractŠedivá J., Zahumenická P., Fernández Cusimamani E. (2017): An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.). Hort. Sci. (Prague), 44: 186-194. This study investigated in vitro production of diploid (AS2) and tetraploid (AS4) cytotypes of snowdrop anemone. The effect of plant growth regulators (PGRs) on in vitro shoot multiplication and rooting was investigated. The effect of activated charcoal (AC) on root induction was also studied. Ploidy level affected growth characteristics during multiplication and rooting. Shoot induction in AS4 was higher on medium supplemented with cytokinin (3.2-3.6), while the AS2 clone formed the most shoots on PGR-free medium (3.6). The highest rooting percentage was achieved on PGRfree medium in both genotypes (AS2 clone, 100% and AS4 clone, 93.3%). The addition of AC to the PGR media largely increased root induction and root length. Rooted plantlets were successfully acclimatised in the greenhouse with 100% survival. Thus, the described micropropagation protocol represents a rapid and effective in vitro propagation method for utilisation in horticulture and conservation programmes of snowdrop anemone.

Micropropagation as a Tool for the Conservation of Autochthonous Sorbus Species of Czechia

“…Regarding the effect of auxin treatment on rooting ability, very interesting results were achieved: in S. gemella , S. × kitaibeliana and S. omissa , better results were obtained in rooting without auxin treatment, excluding S. × abscondita , where the highest rooting percentage was observed in the 2% IBA treatment ( Figure 2 G). A reduction in rooting in the presence of auxin in an agar medium compared to the control (no auxin) has been reported for Anemone L. [ 83 ]. The balance of endogenous growth hormones is a key factor in successful organogenesis in plant tissue cultures.…”