An efficient mixed enzymes-assisted mechanical bio-extraction of polysaccharides from Dendrobium officinale and determination of monosaccharides by HPLC-Q-TOF/MS

Shi, Min-Zhen; Ying, Shen; Jin, Huang-Fei; Cao, Jun

doi:10.1016/j.ijbiomac.2022.11.275

Cited by 10 publications

(1 citation statement)

References 30 publications

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“…Gao et al [ 10 ] extracted E. sibiricumbulb polysaccharides with amylase, and the yield reached 59.71 ± 2.72% after optimization of extraction conditions, which was much higher than that of hot water extraction (37.25 ± 0.17%). Shi et al [ 11 ] extracted Dendrobium polysaccharides with the aid of a combination of cellulase and pectinase, resulting in a much higher extraction rate. Wang et al [ 12 ] extracted pumpkin seed polysaccharides with cellulase and compared the yield with hot water extraction and ultrasonic extraction and concluded that the enzyme extraction (3.22% ± 0.04%) gave a higher yield than hot water extraction (2.18%) and ultrasonic extraction (2.29%).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Enzyme−Assisted Aqueous Extraction of Polysaccharide from Acanthopanax senticosus and Comparison of Physicochemical Properties and Bioactivities of Polysaccharides with Different Molecular Weights

Wang,

Su,

et al. 2023

Molecules

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To obtain the optimal process for the enzyme−assisted aqueous extraction of polysaccharides from Acanthopanax senticosus, and study the physicochemical properties of polysaccharides of different molecular weights, the extraction of Acanthopanax polysaccharides was optimized using the BBD response surface test. The polysaccharides with different molecular weights were obtained by ethanol−graded precipitation at 40%, 60%, and 80%, which were presented as ASPS40, ASPS60, and ASPS80. The polysaccharides were analyzed by HPGPC, ion chromatography, FT−IR, UV, SEM, TGA, XRD, Congo red, and I2−KI tests. The antioxidant assay was used to evaluate their antioxidant properties in vitro. The findings demonstrated that the recovery rate of Acanthopanax polysaccharide was 10.53 ± 0.682%, which is about 2.5 times greater compared to the conventional method of hot water extraction. Based on FT−IR, TGA, polysaccharides with different molecular weights did not differ in their structure or thermal stability. The XRD suggests that the internal structure of ASPSs is amorphous. Congo red and I2−KI showed that all three polysaccharides had triple helix structures with longer branched chains and more side chains. Furthermore, the antioxidant results showed the antioxidant activity of polysaccharides is not only related to the molecular weight size but also can be related to its composition and structure. These studies developed a green, and scalable method to produce polysaccharides from Acanthopanax senticosus and evaluated the properties of Acanthopanax polysaccharides of different molecular weights.

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Section: Introductionmentioning

confidence: 99%

Optimization of Enzyme−Assisted Aqueous Extraction of Polysaccharide from Acanthopanax senticosus and Comparison of Physicochemical Properties and Bioactivities of Polysaccharides with Different Molecular Weights

Wang,

Su,

et al. 2023

Molecules

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The Pre‐ and Post‐Column Derivatization on Monosaccharide Composition Analysis, a Review

Chen,

Wang,

et al. 2024

Chemistry & Biodiversity

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Polysaccharides, as common metabolic products in organisms, play a crucial role in the growth and development of living organisms. For humans, polysaccharides represent a class of compounds with diverse applications, particularly in the medical field. Therefore, the exploration of the monosaccharide composition and structural characteristics of polysaccharides holds significant importance in understanding their biological functions. This review provides a comprehensive overview of extraction methods and hydrolysis strategies for polysaccharides. It systematically analyzes strategies and technologies for determining polysaccharide composition and discusses common derivatization reagents employed in further polysaccharide studies. Derivatization is considered a fundamental strategy for determining monosaccharides, as it not only enhances the detectability of analytes but also increases detection sensitivity, especially in liquid chromatography (LC), capillary electrophoresis (CE), and gas chromatography (GC) techniques. The critical comparison of pre‐ and post‐column derivatization is elaborated in this paper, aiming to serve as a reference for selecting appropriate modes based on the structural characteristics, biological activities of polysaccharides, and reaction systems.

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On-line concentration and separation of multiple derivatized monosaccharides from edible fruit with cyclodextrin-encapsulated sweeping by micellar electrokinetic chromatography

Jin,

Shi,

Shen

et al. 2024

Journal of Chromatography A

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An efficient mixed enzymes-assisted mechanical bio-extraction of polysaccharides from Dendrobium officinale and determination of monosaccharides by HPLC-Q-TOF/MS

Cited by 10 publications

References 30 publications

Optimization of Enzyme−Assisted Aqueous Extraction of Polysaccharide from Acanthopanax senticosus and Comparison of Physicochemical Properties and Bioactivities of Polysaccharides with Different Molecular Weights

Optimization of Enzyme−Assisted Aqueous Extraction of Polysaccharide from Acanthopanax senticosus and Comparison of Physicochemical Properties and Bioactivities of Polysaccharides with Different Molecular Weights

The Pre‐ and Post‐Column Derivatization on Monosaccharide Composition Analysis, a Review

On-line concentration and separation of multiple derivatized monosaccharides from edible fruit with cyclodextrin-encapsulated sweeping by micellar electrokinetic chromatography

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