An efficient regeneration system of barley cultivars from leaf base segments

Li, H. P.; Huang, Tao; Wang, C.; Yuan, Ling

doi:10.1007/s10535-009-0133-9

Cited by 10 publications

(7 citation statements)

References 13 publications

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“…Under light conditions, a purple pigmentation was observed in the leaf tip of buds and shoots. The development of somatic embryos has been demonstrated for many monocots like Pennisetum glaucum (Vasil and Vasil 1980), Zea mays (Lu et al 1983), Paspalum scrobiculatum (Vikrant and Rashid 2003), Paspalum simplex (Molinari et al 2003), Hordeum vulgare (Li et al 2009) and Cenchrus ciliaris (Yadav et al 2009), and mostly primary embryos were formed indirectly via calluses. In B. brizantha, somatic embryo development was similar to the well differentiated apomictic seed embryo as observed by Lenis-Manzano et al (2010).…”

Section: Resultsmentioning

confidence: 99%

“…However, Echinochloa colona (Samantaray et al 1997), maize (Ahmadabadi et al 2007), and barley (Li et al 2009) have been efficiently regenerated from young leaf bases. Moreover, two genotypes of Viburnum dentatum, a popular shrub for landscape use, regenerated shoots when leaf tissues were cultured on woody plant medium (WPM) supplemented with BA or thidiazuron (Dai et al 2011).…”

Section: Somatic Embryogenesis Induction From Basal Segmentsmentioning

confidence: 99%

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Somatic embryogenesis and organogenesis in apomictic and sexual Brachiaria brizantha

Cabral

Carneiro

Lacerda

et al. 2011

Plant Cell Tiss Organ Cult

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Brachiaria brizantha (syn. Urochloa brizantha) is an important tropical forage grass widely cultivated in Brazil. In order to optimize tissue culture conditions for B. brizantha, in vitro culture of mature seeds, basal segments and leaf segments from in vitro plants of an apomictic and a sexual genotype of B. brizantha was performed. When cultured on different media, leaf segments yielded nonembryogenic calluses which formed several roots. Friable calluses from mature seeds and basal segments explants incubated on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine yielded 80% compact and nodular embryogenic structures. Calluses with such compact embryogenic structures were highly regenerable upon transfer to medium supplemented with kinetin and naphthalene acetic acid. They produced isolated somatic embryos, multiple fused scutelli or isolated scutellum with polyembryos that germinated into isolated or multiple shoots. Green and morphologically normal plants were obtained for the two genotypes. Changing the media from pH 5.8 to pH 4.0 increased the number of explants that formed calluses as well as the number of shoots per explant. When embryogenic calluses from mature seeds were successively sub-cultured for 4 months, aiming at repetitive somatic embryogenesis, all the regenerated plants were albinos. The embryogenic nature of the compact structure was confirmed by scanning electron microscopy.

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Section: Resultsmentioning

confidence: 99%

Section: Somatic Embryogenesis Induction From Basal Segmentsmentioning

confidence: 99%

Somatic embryogenesis and organogenesis in apomictic and sexual Brachiaria brizantha

Cabral

Carneiro

Lacerda

et al. 2011

Plant Cell Tiss Organ Cult

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“…nudum). Successful formation of embryogenic calli from leaf base segments of seven semi-winter, commercial cultivars from China and following regeneration was recently reported (Li et al 2009). …”

Section: Barley Cultivarsmentioning

confidence: 99%

Genetic transformation of barley: limiting factors

Vyroubalová¹,

Šmehilová²,

Galuszka³

et al. 2011

Biologia plant.

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This review summarizes main difficulties involved in barley (Hordeum vulgare L.) transformation. The most commonly used procedures for genetic transformation in barley are Agrobacterium tumefaciens and particle bombardment mediated methods. While different barley cultivars are used for genetic engineering with varying sensitivity, recent improvements in regeneration and transformation techniques are described and summarized. Furthermore, some of the transformation complicating factors, in particular somaclonal variation and transgene insertion sites, are discussed in more detail.

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“…The majority of methods for their micropropagation are callus-mediated and genotype-dependent. Studies have shown in vitro regeneration from shoot apices [4][5][6], meristematic segments of young seedlings [7][8][9] and leaf bases [10]. Some authors have reported the regeneration of adventitious shoots from enlarged shoot apical meristems without a callus phase [3][4][5][6][7] or with only a minimal callus phase [9].…”

Section: Introductionmentioning

confidence: 99%

The effect of genotype on a barley scutella culture. Histological aspects

et al. 2013

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Cereals are known to be recalcitrant to the induction of morphogenesis in vitro and the majority of the methods used are callus-mediated and species/genotype-dependent. In the present investigation, a method of morphogenesis induction from immature scutella of selected barley cultivars was used, and particular attention was paid to histology in the initial stages of the regeneration process in order to confirm whether it occurs directly or indirectly (via callus formation). The length of the period from inoculating scutella on the medium to obtaining plantlets depended on the cultivar and the individual scutellum of the barley and varied between 2.5–4 months. The regeneration efficiency and viability of barley scutella was revealed to be highly genotype dependent. The average number of regenerated plants per regenerating scutellum was highest in the case of cv Granal (3.7). A histological analysis of the cultured explants showed both non-morphogenic and morphogenic callus formation. Two types of indirect morphogenetic response were observed: organogenesis (shoot bud formation) and somatic embryogenesis. This is the first report concerning an analysis of in vitro regeneration from immature scutella of barley cultivars (Stratus, Ryton, Granal and Binal).

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An efficient regeneration system of barley cultivars from leaf base segments

Cited by 10 publications

References 13 publications

Somatic embryogenesis and organogenesis in apomictic and sexual Brachiaria brizantha

Somatic embryogenesis and organogenesis in apomictic and sexual Brachiaria brizantha

Genetic transformation of barley: limiting factors

The effect of genotype on a barley scutella culture. Histological aspects

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