An efficient regeneration system via somatic embryogenesis in mango ginger (Curcuma amada Roxb.)

Raju, Chellappan Soundar; Kathiravan, K.; Aslam, Abubakker; Shajahan, Appakan

doi:10.1007/s11240-012-0244-4

Cited by 36 publications

(17 citation statements)

References 39 publications

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“…However, monocotyledonous plants are generally known to be recalcitrant towards somatic embryogenesis. In the genus Curcuma, there are very few reports to date on protocols for efficient and reliable regeneration system via somatic embryogenesis (Raju et al, 2013;He & Gang, 2014). Thorough understanding of the genetic mechanisms underlying various aspects of somatic embryogenesis could help overcome the barriers to the process and thus lead to successful implementation of the technology in in vitro propagation of Curcuma species, including C. alismatifolia.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Expression Analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) Gene in Curcuma alismatifolia Gagnep.

Sucharitakul¹,

Rakmit²,

Boonsorn³

et al. 2014

JAS

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Somatic embryogenesis provides a useful tool to facilitate efficient mass propagation in plants. The SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) gene serves a fundamentally important role in somatic embryogenesis of many plant species. The isolation of a SERK gene homolog, namely CaSERK, from Curcuma alismatifolia Gagnep. cv. Blue Tung, was reported. Prediction of coding sequence showed that it encoded a protein of 628 amino acids showing high similarity to previously characterized SERK sequences and containing all the features shared by members of the SERK family, including five leucine-rich repeats and the distinctive proline-rich SPP domain. Investigation of CaSERK expression revealed that its transcripts were found throughout the whole somatic embryogenesis process with highest abundance in embryogenic callus. These results indicate that CaSERK might have somatic embryogenesis-associated functions in this economically important ornamental ginger. Detection of CaSERK transcript accumulation in flower and coma bract tissues is suggestive of its additional roles in other developmental signaling pathways.

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Section: Introductionmentioning

confidence: 99%

Isolation and Expression Analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) Gene in Curcuma alismatifolia Gagnep.

Sucharitakul¹,

Rakmit²,

Boonsorn³

et al. 2014

JAS

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“…A change in strength directly influenced the formation and maturation of somatic embryos (Komatsuda et al 1992). In C. amada, somatic embryogenesis was better on half-strength MS medium (Soundar Raju et al 2013). This is probably because, prevalence of optimal concentration of inorganic nutrients in the culture medium results in a favorable optimal osmotic potential for dedifferentiation of embryogenic callus.…”

Section: Role Of Osmoticummentioning

confidence: 96%

“…Tissue culture studies in C. amada have shown mostly adventitious plantlet formation from rhizome and leaf sheath explants (Prakash et al 2004;Das et al 2010;Banerjee et al 2012). This attempt aims to elaborate on somatic embryogenesis in C. amada as studied in our laboratory (Soundar Raju et al 2013, 2014.…”

Section: Propagationmentioning

confidence: 99%

“…Prakash et al (2004) obtained semi-friable organogenic callus from leaf sheath explants of C. amada on MS medium containing 2,4-D alone. Soundar Raju et al (2013) suggested that the MS medium supplemented with 2,4-D in combination with BA is a prerequisite for white friable embryogenic callus formation in C. amada. 2.0 mg/L 2,4-D and 0.5 mg/L BA was found to be the most efficient (26.43 %) for the induction of embryogenic calli from leaf sheath explants ( Fig.…”

Section: Medium For Callus Inductionmentioning

confidence: 99%

“…Soundar Raju et al (2013) found that the embryogenic callus subcultured in medium containing BA gave best results on somatic embryogenesis in C. amada.…”

Section: Induction Of Somatic Embryogenesismentioning

confidence: 99%

See 2 more Smart Citations

Direct and Indirect Somatic Embryogenesis in Mango Ginger (Curcuma amada Roxb.)

Shajahan

Raju

Thilip

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

Self Cite

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Somatic embryogenesis is a developmental restructuring of somatic cells in the embryogenic pathway and forms a basis for the concept of totipotency in plant cells. Understanding the process of transition from vegetative to embryogenic cells and factors involved in the somatic embryogenesis and subsequent plant development represents a challenge for plant tissue culture studies in any selected species. Although several reviews have been published dealing somatic embryogenesis in several plant species, there is no recent information on somatic embryogenesis in mango ginger (Curcuma amada). Two different types of somatic embryogenetic pathways are available in mango ginger. They are indirect somatic embryogenesis (IDSE) and direct somatic embryogenesis (DSE). Indirect somatic embryogenesis occurs in friable embryogenic callus from leaf sheath explants that undergo an extreme proliferation before the development of somatic embryos, whereas in the direct somatic embryogenesis, two-step system of culture was followed. Initially, leaf sheath explants were subjected to a pretreatment under dark condition before they were transferred to the subculture medium for embryo development. Histological and scanning microscopic studies showed that the formation of translucent globular embryogenic cell suspension is an important visible stage during early somatic embryogenesis through indirect method. In the direct pathway, upon pretreatment, epidermal and subepidermal cells of the leaf sheath explants formed globular and elongated structures. The presence of clear protoderm in the globular embryos and procambial structures are crucial visible stages.

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