An efficient ribitol-specific dehydrogenase from Enterobacter aerogenes

Cloning and characterization of a new ribitol dehydrogenase fromProvidencia alcalifaciensRIMD 1656011

“…Other RDHs such as those isolated from Z. mobilis subsp. mobilis ZM4, E. agglomerans strain 221e, E. aerogenes and R. sphaeroides Si4 showed optimum temperatures of 65, 50–60, 45 and 45 °C respectively.…”

“…The highest activity was achieved when the enzyme was incubated with Ba 2+ . Regarding RDHs from other organisms, the activity of RDH from Z. mobilis was markedly enhanced by Mn 2+ , while the activity of RDH from E. aerogenes was found to be dependent on the following metal ions: Ca 2+ , Ba 2+ , Mg 2+ , Mn 2+ and Co 2+ . RDHs from different organisms have different metal ion dependences as shown in Table .…”

“…The recombinant enzyme was purified by affinity chromatography. The molecular mass results of recombinant P. alcalifaciens RDH suggested that P. alcalifaciens RDH oligomerizes to form a tetramer with four identical subunits, compared with previously reported RDHs with a predicted molecular mass ranging between 25 and 28 kDa and a quaternary structure varying between tetramer and dimer, while a tetramer quaternary structure was reported for RDHs isolated from E. agglomerans , G. oxydans , E. aerogenes and K. aerogenes . Table shows a comparison of the kinetic and biochemical properties between RDH from P. alcalifaciens and RDHs from other microorganisms.…”

Allitol: production, properties and applications

“…RDHs have been isolated from various sources, such as Aerobacter aerogenes (Fossitt & Wood, 1966), Enterobacter agglomerans (Muniruzzaman et al, 1995b), Gluconobacter oxydans (Adachi et al, 2001), Klebsiella aerogenes (Taylor et al, 1974), Rhodobacter sphaeroides (Kahle et al, 1992), Klebsiella oxytoca (Han et al, 2104), Z. mobilis (Moon et al, 2010), E. aerogenes (Singh et al, 2015) and Providencia alcalifaciens (Hassanin et al, 2015) (Table 2). In addition, RDH catalyses production of D-ribulose from ribitol.…”

“…The Michaelis-Menten constants (K m ) of RDHs from K. aerogenes, Rhodobacter sphaeroides, E. agglomerans, G. oxydans, Z. mobilis, E. aerogenes and P. alcalifaciens with ribitol were 8, 6.3, 32.2, 1.2, 11.8, 10.3 and 13.9 mM, respectively (Kahle et al, 1992;Muniruzzaman et al, 1995b;Adachi et al, 2001;Moon et al, 2010;Han et al, 2014;Hassanin et al, 2015;Singh et al, 2015). No previous study has examined the kinetic parameters of reduction in D-psicose into allitol by RDH.…”