An efficient strategy overcoming the bottleneck in Candida parapsilosis catalyzing stereoinversion from (R)‐1‐phenyl‐1, 2‐ethanediol to the corresponding counterpart

Abstract

BACKGROUND: Microbial stereoinversion has been widely used for the biosynthesis of numerous chiral compounds. However, little work has been done to improve the efficiency of microbial stereoinversion. This study investigated the bottleneck in the deracemization of 1-phenyl-1,2-ethanediol (PED), and then the efficiency and the sustainability of biocatalyst was improved significantly by using a strategy.

Reactive Separations for In Situ Product Removal of Enzymatic Reactions: A Review

“…The focus was mainly on the application of in situ extraction in fermentation processes, as can be found in former reviews [16,[136][137][138][139][140]. This early focus on extraction can be explained by benefits discussed earlier, like avoiding toxicity effects [4][5][6][7] or inhibition of the enzyme [7,8,136,[141][142][143][144]. Beside this, a second liquid phase can be used as a reservoir for substrates [145][146][147][148][149] or recycling of the homogeneous catalyst [150].…”