An electrochemical aptasensor based on cocoon-like DNA nanostructure signal amplification for the detection of Escherichia coli O157:H7

Abstract

We developed an electrochemical aptasensor based on cocoon-like DNA nanostructures as signal tags for highly sensitive and selective detection of Escherichia coli O157:H7. The stable cocoon-like DNA nanostructures synthesized by...

Advances in Electrochemical Aptamer Biosensors for the Detection of Food‐borne Pathogenic Bacteria

“…Similarly, Bai et al [35] developed a sensor for E. coli O157:H7 (e. g. Figure 3), applying rolling cycle amplification (RCA) to produce cocoon-like DNA nanostructures (CDNs) containing hemin tags to be used as signal-labelled probes to amplify electrical signals. This synthetic DNA nanostructure allows for the programmability of the nucleic acid sequence.…”

Recent Advances and a Roadmap to Aptamer-Based Sensors for Bloodstream Infections

“…Another sensor was developed for detection of E. coli O157:H7 based on cocoonlike DNA nanostructures. 209 These nanostructures were loaded with hemin as electrochemical signal tags to amplify the signals (section C of Figure 8). Further an electrochemical aptasensor was developed using electrochemically reduced graphene oxide-chitosan (rGO−CHI) composite as a conductive substrate to detect whole-cell Salmonella enterica serovar Typhimurium 230 (section D of Figure 8).…”

“…An electrochemical aptasensor using a screen-printed electrode modified with reduced graphene oxide (rGO) and gold nanourchins (AuNUs) was developed for Staphylococcus enterotoxin B ( SEB ) detection (section B of Figure ). Another sensor was developed for detection of E. coli O157:H7 based on cocoon-like DNA nanostructures . These nanostructures were loaded with hemin as electrochemical signal tags to amplify the signals (section C of Figure ).…”

A DNA tetrahedral nanomaterial-based dual-signal ratiometric electrochemical aptasensor for the detection of ochratoxin A in corn kernel samples

“…For instance, tetrahedral and other DNA nanostructures are often assembled on the electrode surface through one-step thermal denaturation. 32,33 Among them, the DNA nanotetrahedron (NTH) with a spatial structure has a stable and rigid structure that can precisely locate the orientation of the probe and obliterate the non-specific binding of DNA and target on the substrate. 34,35 Ultimately, 3D DNA NTH facilitates the efficient recognition of the target to improve the precision and accuracy of the detection method.…”