An electrochemical aptasensor for the milk allergen β-lactoglobulin detection based on a target-induced nicking site reconstruction strategy

Abstract

Food allergy is an immune system reaction to a particular food, in which milk is the most common one. β-lactoglobulin (β-Lg) is the main ingredient of milk protein and the...

Fluorescence detection of milk allergen β-lactoglobulin based on aptamers and WS2 nanosheets

“…The dissolved solution was heated to 40 °C and held 30 min; then centrifuged at 15,000 rpm for 15 min. 39 The supernatant was acidified to pH 4.6 with 2 M HCl. After 20 min, the supernatant was centrifuged at 8000 rpm for 20 min.…”

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

“…Recently, the Chen group has developed an aptasensor for β-LB detection based on a tri-functional hairpin (HP) [ 119 ]. In particular, HP included an appropriate aptamer part, a nicking site with two complementary sequences named site-1 and site-2 and a DNA sequence (named as T1) for amplification.…”

Electrochemical Methods for the Analysis of Milk

“…Other electrode chemical modifications can be obtained by deposition of metal nanoparticles, ,,− conducting polymers, ,,, and biological active modifiers such as enzymes ,, and aptamers ,,,, on the electrode surface to create a biosensor. Deposition of metal nanoparticles such as nanorods, nanowires, or nanosheets facilitate particular reactions and increase the electron transfer rate between the analyte and electrode. ,− Besides, metal nanoparticles such as gold nanoparticles can be an excellent scaffold for specific attachments for DNA or proteins (e.g., antibody, enzyme, etc.)…”

“…Deposition of metal nanoparticles such as nanorods, nanowires, or nanosheets facilitate particular reactions and increase the electron transfer rate between the analyte and electrode. ,− Besides, metal nanoparticles such as gold nanoparticles can be an excellent scaffold for specific attachments for DNA or proteins (e.g., antibody, enzyme, etc.) in biosensors . Conducting polymers are sensor building materials due to their unique physical and chemical properties, stability, structural versatility, and semiconductivity. , Polypyrroles, polythiophenes, and polyanilines are the most recurrent conducting polymers made from biofunctional precursor monomers resulting in linear and branched polymer nanostructures .…”

“…), − pesticides (e.g., organochlorine paraoxan), fungicides (e.g., carbendazim), insecticides residues (e.g., parathion), heavy metals (lead, cadmium, etc. ), , and milk allergens (β-lactoglobulin). , Another concern is the availability of suitable techniques to detect and quantify these chemicals in a fast, sensitive, and accurate manner at all stages of milk production, from cattle feeding to milk collection, storage, distribution, and sale.…”