An Electrochemical Biosensor for SARS-CoV-2 Detection Via its Papain-Like Cysteine Protease and the Protease Inhibitor Screening

Molecularly imprinted miniature electrochemical biosensor for SARS-CoV-2 spike protein based on Au nanoparticles and reduced graphene oxide modified acupuncture needle

“…Reverse transcription-polymerase chain reaction (RT-PCR) [3] and the detection of IgG/IgM in whole blood or serum [4] are the two main methods used for the diagnosis of SARS-CoV-2. However, RT-PCR has inevitable limitations such as high cost, time-consumption and trained personnel requirement [5] , while the detection of IgG/IgM has little significance for clinical diagnosis due to the positive specific antibodies in vaccinated individuals [6] . Recently, the detection of SARS-CoV-2 specific antigens such as nucleoprotein [7] , [8] and spike protein [9] , [10] has provided the possibility of early diagnosis of SARS-CoV-2.…”