An electrochemical DNA biosensor based on nitrogen-doped graphene/Au nanoparticles for human multidrug resistance gene detection

Chen, Mei; Hou, Changjun; Huo, Dehong; Bao, Jing; Fa, Huanbao; Shen, Caihong

doi:10.1016/j.bios.2016.05.051

Cited by 112 publications

(45 citation statements)

References 31 publications

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“…In their model, the decreased output signal represented perfect match (Off), while a mismatch gives an opposite signal (On). In this context our results are in line with other studies [17,18,24], as hybridization of CC amplicons with the specific MGB-probe presented a lower current, with a decreased on redox current peaks as compared to TT amplicons. In this context our results are in line with other studies [17,18,24], as hybridization of CC amplicons with the specific MGB-probe presented a lower current, with a decreased on redox current peaks as compared to TT amplicons.…”

Section: Ultrasensitive Genosensor Based On Minor Grove Binding (Mgb)supporting

confidence: 92%

“…In label-free electrochemical genosensor approach, the use of DNA intercalating redox probes has been described [16,17]. DNA hybridization event using methylene blue as an electrochemical indicator have been used to detect human multidrug resistance gene [18]. However, this system presents a low sensitivity to detect one mismatch, SNPs.…”

Section: Ultrasensitive Genosensor Based On Minor Grove Binding (Mgb)mentioning

confidence: 99%

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Ultrasensitive Genosensor Based on Minor Grove Binding (MGB) Probe for IL28B Single Nucleotide Polymorphism (SNP) Detection Using SYBR Green as Electrochemical Indicator

2018

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A genosensor for typing the IL28B single nucleotide polymorphism (rs12979860), an important marker predictive of the treatment response to hepatitis C (HCV) infection was developed. The electrochemical sensor was based on a nanohybrid poly‐pyrrole‐carbon multiwall nanotubes film functionalized with minor groove binding (MGB) oligonucleotides probes to allele C of the IL28B. Individuals with genotype CC are more likely to achieve the virus clearance after HCV treatment (60 %), while homozygous TT (variant) is associated to a poor therapy response (20 %). A conventional SYBR green PCR was performed to obtain amplicons of CC and TT genotypes to test the DNA binding response of an MBG‐genosensor device. The redox response was monitored as hybridization match arose between probes and amplicons. The results showed a significant increase in the current redox peaks after TT genotype amplicons incubations in comparison to CC genotypes, ascribed to the weak SYBR Green intercalation between strands, and exposing the its reactive groups, favouring an increased electrocatalytic response. In conclusion, it was presented a new architecture of a genosensor with On‐Off polarity exploring the high specificity and sensitivity of MGB‐probes with promise application for single nucleotide polymorphism, using Syber Green as electrochemical indicator.

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Section: Ultrasensitive Genosensor Based On Minor Grove Binding (Mgb)supporting

confidence: 92%

Section: Ultrasensitive Genosensor Based On Minor Grove Binding (Mgb)mentioning

confidence: 99%

Ultrasensitive Genosensor Based on Minor Grove Binding (MGB) Probe for IL28B Single Nucleotide Polymorphism (SNP) Detection Using SYBR Green as Electrochemical Indicator

2018

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“…The current trend in DNA sensing technology mostly involves heterogeneous hybridization between a surface‐bound single stranded DNA with known genomic sequence (the probe) and a free target DNA with complementary/mismatched genomic sequence. Common transduction techniques associated with DNA biosensing mostly include the use of optical, mass‐sensitive and electrochemical methods . Additionally, several advanced methods have also been developed in the past few years that have contributed to the advancements in cancer research .…”

Section: Introductionsupporting

confidence: 58%

Graphene Doped Mn₂O₃ Nanofibers as a Facile Electroanalytical DNA Point Mutation Detection Platform for Early Diagnosis of Breast/Ovarian Cancer

et al. 2018

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This paper demonstrates a simple, label‐free detection methodology for detecting single point DNA mutations. Single point mutation detection is a key enabler for diagnosis and prevention of several genetic disorders that manifest into cancers. Specifically for this purpose, herein, an electrochemical biosensor utilizing electrospun graphene doped manganese III oxide nanofibers (GMnO) is developed. The charge transfer resistance offered by GMnO is extremely sensitive to the localized change in the conductivity. This sensitivity, attributed to the low band gap of Mn2O3 and high charge transfer kinetics of graphene, is explored in the proposed mutation detection platform. As a proof of concept, ultrasensitive detection of BRCA1 gene specific point mutation is demonstrated. The target specific single stranded probe DNA is immobilized onto GMnO modified glassy carbon working electrodes via chemisorption. Post target‐DNA hybridization, differential pulse voltammetry is employed to facilitate detection of targeted point mutation, wherein, difference in peak currents is used to distinguish the target DNA as normal or mutant. Efficiency of the proposed method is evaluated against a target concentration ranging from 10 pM−1 μM. With respect to the mutated target DNA, the LoD of the proposed device is found to be 0.8±0.069 pM. The proposed approach can be extended for detecting any mutation/hybridization of interest by simply adapting an appropriate functionalization protocol.

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“…It demonstrates that the prepared biosensor can provide a rapid detection process. Table 1 shows the comparison of several methods for determination of DNA [38][39][40]. The results derived from the PEC biosensor are corresponded to the existing methods currently.…”

Section: Photoeletrochemical Detection Of Pec Biosensormentioning

confidence: 94%

A photoelectrochemical biosensor for determination of DNA based on flower rod-like zinc oxide heterostructures

et al. 2017

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The work describes a photoelectrochemical (PEC) biosensor for the detection of DNA. It employs oriented hierarchical ZnO flower-rod architectures (ZnO FRs) and DNA dendrimers. ZnO FRs act as photoactive material that yields a photocurrent of up to 23 μA. The photogenerated electron transfer is inhibited once the probe DNA and the target DNA oligonucleotides hybridize, and this results in a reduced photocurrent. The use of DNA dendrimers with scores of DNA branches further amplifies the signal of the PEC biosensor. The PEC sensor displays a response that is linear in the DNA concentration range from 10 fM to 0.1 μM with a detection limit of 3.7 fM (at S/N = 3). The sensor was applied to the determination of DNA in human serum samples and was found to work with acceptable accuracy. Due to the use of ZnO FRs and DNA dendrimers, the assay is highly sensitive, rapid, and repeatability.

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An electrochemical DNA biosensor based on nitrogen-doped graphene/Au nanoparticles for human multidrug resistance gene detection

Cited by 112 publications

References 31 publications

Ultrasensitive Genosensor Based on Minor Grove Binding (MGB) Probe for IL28B Single Nucleotide Polymorphism (SNP) Detection Using SYBR Green as Electrochemical Indicator

Ultrasensitive Genosensor Based on Minor Grove Binding (MGB) Probe for IL28B Single Nucleotide Polymorphism (SNP) Detection Using SYBR Green as Electrochemical Indicator

Graphene Doped Mn₂O₃ Nanofibers as a Facile Electroanalytical DNA Point Mutation Detection Platform for Early Diagnosis of Breast/Ovarian Cancer

A photoelectrochemical biosensor for determination of DNA based on flower rod-like zinc oxide heterostructures

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An electrochemical DNA biosensor based on nitrogen-doped graphene/Au nanoparticles for human multidrug resistance gene detection

Cited by 112 publications

References 31 publications

Ultrasensitive Genosensor Based on Minor Grove Binding (MGB) Probe for IL28B Single Nucleotide Polymorphism (SNP) Detection Using SYBR Green as Electrochemical Indicator

Ultrasensitive Genosensor Based on Minor Grove Binding (MGB) Probe for IL28B Single Nucleotide Polymorphism (SNP) Detection Using SYBR Green as Electrochemical Indicator

Graphene Doped Mn2O3 Nanofibers as a Facile Electroanalytical DNA Point Mutation Detection Platform for Early Diagnosis of Breast/Ovarian Cancer

A photoelectrochemical biosensor for determination of DNA based on flower rod-like zinc oxide heterostructures

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Graphene Doped Mn₂O₃ Nanofibers as a Facile Electroanalytical DNA Point Mutation Detection Platform for Early Diagnosis of Breast/Ovarian Cancer