An Electrochemical DNA Biosensor for the Detection of the Apa I Polymorphism in the Vitamin D Receptor Gene Using Meldola's Blue as a Hybridization Indicator

Aladağ, Nilay; Ozkan-Ariksoysal, Dilsat; Gezen-Ak, Duygu; Yılmazer, Selma; Ozsoz, Mehmet

doi:10.1002/elan.200900405

Cited by 9 publications

(7 citation statements)

References 55 publications

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“…Vyskyocil et al 196 used a DNA biosensor based on a screen printed CPE immobilized with double-stranded DNA for in detection of genotoxic nitro derivatives. Aladag et al 197 used DNA biosensors to detect Apa I polymorphism, which are involved in Alzhiemer’s Disease, within 30 minutes, which is more rapid than other current assays. Zhang et al 198 used DNA biosensors for simultaneous detections of both HIV-1 and HIV-2 oligonucleotides.…”

Section: Biosensorsmentioning

confidence: 99%

Electrochemical Sensors and Biosensors

et al. 2011

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By using a solid KCl melt in conjunction with an Ag/AgCl component, Vanau et al. 8 were able to fabricate an SSRE designed specifically for beverage industry applications. They found that this SSRE had a wide range of pH use, stable potentials, and small drift potentials (1 mV at room temperature over 3 months time). The reported stability and lifetime of this reference electrode make it particularly applicable for the food industry. In addition to food industry, engine diagnostics are utilizing SSRE to assess efficiency. Oxygen sensors have long been utilizing yttria-stabilized zirconia-based potentiometric components. Recently, Mn-based oxides have been studied to find a suitable SSRE to ensure accurate on-board diagnosis for engines. Miura et al. 9 found Mn 2 O 3 -sensing electrodes to function with excellent sensitivity and has great potential for miniaturization.Rius-Ruiz et al. 10 recently reported a carbon nanotube (CNT)-based SSRE. The most successful design tested utilized a photopolymerized n-butyl acrylate polymer in conjuction with SWCNTs, acting as the transducer layer. This type of transducer layer was often superior to alternative solid transducers, and the resulting SSRE proved insensitive to room lighting. The superior characteristics of this reference electrode as well as its ease of fabrication allow for potential widespread usage in a multitude of systems.Other Papers of Interest. To ensure reference electrodes keep up with current miniaturization trends, it has become challenging to find suitable materials for all potential applications. One such challenging arena is that of steel corrosion monitoring. Muralidharan et al. 11 developed a NiFe 2 O 4 reference electrode due to its superior fabrication cost and ease and its resistance to corrosion. The resulting electrode exhibited stability and low polarization currents in a calcium hydroxide solution, which is commonly used in concrete environments.Shibata et al. 12 examined the stability of an Ag/AgCl reference electrode with the novel salt bridge 1-methyl-3-octylimidazolium bis(trifluoromethanesulfonyl)-amide. This bridge was proposed and found to be superior in order to avoid the interference found using alternate ionic liquid salt bridges, such as KCl, while experimenting in phthalate buffer. Further evaluation of stability is necessary; however, the initial findings suggest this ionic liquid salt bridge is promising. Huang et al. 13 recently developed a working system using a Nafion strip membrane as an ion-conducting bridge, allowing for electrochemical measurements in pure water. By using this bridge, it is possible to avoid potential leaching contaminants and gain higher accuracy.Measuring the electrodeposition of boron melts is a challenge to researchers due to the unknown chemistry of KF-KCl-KBrF 4 . Pal et al. 14 fabricated an Ag/AgCl reference electrode that was found to be reversible and to have suitable stability and nonpolarizability. This will allow for the first available measurements of KF-KCl-KBrF 4 chemistry at high tempera...

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Section: Biosensorsmentioning

confidence: 99%

Electrochemical Sensors and Biosensors

et al. 2011

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“…However, the reduction signal of MDB increases following the hybridization because MDB can selectively bind to duplex form of DNA. 13 In addition, when using PGA coated electrode, biosensor has shown superior electrocatalytic activity on MDB reduction signal (Figure 2).…”

Section: Resultsmentioning

confidence: 95%

“…Following hybridization, the DNA biosensors were immersed for 5 min in 20 mM tris buffer solution (TBS) (pH 7.0) containing 20 mM MDB. 12,13 Then, the biosensors were immersed in washing buffer (1×SSC+ 0.1% SDS, pH 7.0) for 5 min. The DNA sensor was then dipped into the TBS (pH 7.0) for voltammetric measurement.…”

Section: Interaction With the Intercalator Molecule And Transduction mentioning

confidence: 99%

Development of an electrochemical DNA biosensor for detection of specific Mycobacterium tuberculosis sequence based on poly(L-glutamic acid) modified electrode

2016

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An electrochemical DNA biosensor was developed by avidin-biotin interaction of a biotinylated probe and avidin-attached, poly(L-glutamic) acid coated pencil graphite electrode (PGA/PGE) for detection of specific Mycobacterium tuberculosis DNA sequence. The discrimination of fully complementary hybridization and mismatch hybridization was carried out by electrochemical reduction current of Meldola's Blue (MDB) in square wave voltammetry (SWV). The calibration graph of the DNA biosensor was linear between 1.5-12.5 nM and the detection limit was calculated as 1.3 nM. The proposed biosensor successfully discriminated short and long oligonucleotides related to DNA sequence of Mycobacterium tuberculosis in optimal condition.

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“…These mutations can be used to trace generational inheritance patterns associated with specific diseases [55]. In addition, the developed biosensor compares favorably to other DNA biosensors based on a similar method for the hybridization detection (Table 2SI), considering important analytical properties, such as sensitivity and detection limit [51,43,[56][57][58][59]. Scheme 2.…”

Section: Screening Of Specific Gene Mutations Associated With Cystic mentioning

confidence: 99%

Carbon nanodots based biosensors for gene mutation detection

García‐Mendiola

Bravo

López-Moreno

et al. 2018

Sensors and Actuators B: Chemical

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An electrochemical DNA biosensor based on a carbon nanodots (CDs) modified screenprinted gold electrode as a transducer is reported in this work. CDs were synthesized by thermal carbonization of ethyleneglycol bis-(2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) and characterized by different techniques (DLS, TEM, FTIR, Raman). The electrode surface modification was accomplished by drop-casting a suspension of CDs. SEM analysis and cyclic voltammetry were used to characterize the resulting modified electrode. Synthetic 25-mer or 100-mer DNA capture probes, capable to hybridize with a specific sequence of the pathogen Helicobacter pylori or the cystic fibrosis transmembrane regulator (CFTR) gene were attached to the CDs-gold surface. A 25-bases synthetic fully complementary sequence or a single nucleotide polymorphism to the DNA capture probe and a 373-bases PCR amplicon of exon 11 of CFTR containing a sequence complementary to the capture probe, were employed as target. The hybridization event was electrochemically monitored by using safranine as redox indicator, which selectively binds to double stranded DNA (dsDNA). A

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An Electrochemical DNA Biosensor for the Detection of the Apa I Polymorphism in the Vitamin D Receptor Gene Using Meldola's Blue as a Hybridization Indicator

Cited by 9 publications

References 55 publications

Electrochemical Sensors and Biosensors

Electrochemical Sensors and Biosensors

Development of an electrochemical DNA biosensor for detection of specific Mycobacterium tuberculosis sequence based on poly(L-glutamic acid) modified electrode

Carbon nanodots based biosensors for gene mutation detection

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