An electrochemical DNA sensor for sequence-specific DNA recognization in a homogeneous solution

A novel photoelectrochemical sensor based on photocathode of PbS quantum dots utilizing catalase mimetics of bio-bar-coded platinum nanoparticles/G-quadruplex/hemin for signal amplification

“…The detection limit was 0.08 pM. This detection limit was not only much lower than other assays for the detection of target DNA with different measurement protocols such as electrochemistry (Wan et al, 2014;Cui et al, 2014;Dharuman and Hahn, 2008), fluorimetry Sun et al, 2014;Sharon et al, 2010;Zhang et al, 2013), colorimetry (Nie et al, 2014), but also much lower than those PEC detection with different amplification strategies (Golub et al, 2012;Liu et al, 2006Liu et al, , 2008. The detailed comparison for different methods is listed in Table S2.…”

Electrochemical DNA Biosensors Based on Labeling with Nanoparticles

“…Numerous processes on the synthesis of NPs and QDs have been introduced and reviewed recently [4,13]. Regarding DNA applications, the typical synthesis of AuNPs relies on the reduction of Au(III) (from hydrogen tetrachloroacurate, HAuCl 4 ) to Au(0) in the presence of a capping agent, such as sodium borohydride, D-glucose, or sodium citrate—which is the most applied [4,13,25,26,27,28]. According to these protocols, an aqueous solution of HAuCl 4 is boiled in a conical flask under stirring.…”

Ag(I)-coordinated hairpin DNA for homogenous electronic monitoring of hepatitis C virus accompanying isothermal cycling signal amplification strategy

“…Lei et al (2014) reported a new application of supercharged proteins as a versatile biosensing platform for homogeneous DNA detection and methylation analysis. Unfortunately, most of the existed homogeneous assay formats for nucleic acids are based on the fluorescencebased signal-transduction principles, and there are a few reports focusing on electrochemical measurement (Miranda-Castro et al, 2012;Luo and Hsing, 2010;Cui et al, 2014;Liu et al, 2014;Nie et al, 2014;Xuan et al, 2012). One important challenge for development of homogeneous electrochemical DNA sensors is the readout method since the electronic signal mainly originates from the redox reaction of electroactive indicators on the electrode surface (i.e., the solid-solution interface).…”