An electrochemical immunosensor for sensitive detection of Escherichia coli O157:H7 using C60 based biocompatible platform and enzyme functionalized Pt nanochains tracing tag

Li, Yan; Fang, Lichao; Cheng, Ping; Deng, Jun; Jiang, Lili; Huang, Hui; Zheng, Junsong

doi:10.1016/j.bios.2013.06.008

Cited by 68 publications

(38 citation statements)

References 29 publications

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“…A complex amperometric sensor was constructed to detect heat-killed E. coli strains spiked into synthetic stool samples (78). First, a biocompatible nanolayer of fullerene (C60), ferrocene (Fc), and thiolated chitosan (CHI-SH) composite was deposited on top of glassy carbon electrodes, followed by conjugation of Au-SiO 2 -streptavidin-biotinyl primary antibodies.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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SUMMARY Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, and security. Bacterial infection is a common cause of morbidity and mortality worldwide. In spite of the availability of antibiotics, these infections are often misdiagnosed or there is an unacceptable delay in diagnosis. Current methods of bacterial detection rely upon laboratory-based techniques such as cell culture, microscopic analysis, and biochemical assays. These procedures are time-consuming and costly and require specialist equipment and trained users. Portable stand-alone biosensors can facilitate rapid detection and diagnosis at the point of care. Biosensors will be particularly useful where a clear diagnosis informs treatment, in critical illness (e.g., meningitis) or to prevent further disease spread (e.g., in case of food-borne pathogens or sexually transmitted diseases). Detection of bacteria is also becoming increasingly important in antibioterrorism measures (e.g., anthrax detection). In this review, we discuss recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing. There is a particular focus on electrochemical biosensors, especially impedance-based systems, as these present key advantages in terms of ease of miniaturization, lack of reagents, sensitivity, and low cost.

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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“…However, this hollow carbon sphere, rich in conjugated π electrons with excellent electron acceptor capability to accelerate electron transfer [1,8,13], can react with amines [14] and be successfully decorated with other materials [6,12,[15][16][17]. This explains the increasing interest in the preparation of C 60 composites with other nanomaterials to achieve new physical and chemical properties especially relevant for the application of C 60 in novel electrode materials and the fabrication of sensors and biosensors [18].…”

Section: Fullerenes In Electrochemical Biosensingmentioning

confidence: 99%

“…This explains the increasing interest in the preparation of C 60 composites with other nanomaterials to achieve new physical and chemical properties especially relevant for the application of C 60 in novel electrode materials and the fabrication of sensors and biosensors [18]. Indeed, the combination of C 60 with nanomaterials such as nitrogen-doped carbon nanotubes (NCNTs) [18], AuNPs [1,6,15,19], and Pt-Pd nanoparticles [17] has been shown to be advantageous either because combining the unique properties of these two kinds of materials or the emergence of new properties leads to novel applications [12]. Moreover, C 60 owned its inner redox activity [15], being able to act as an electron acceptor with a dual nature of electrophilic and nucleophilic characteristics, which has led to significant interest from researchers to investigate the possibilities of using this material as a mediator in biosensing devices [4,15,20].…”

Section: Fullerenes In Electrochemical Biosensingmentioning

confidence: 99%

“…The following subsections show as C60 and their derivatives have been used as electrode modifiers in the design of electrochemical affinity biosensors [1,5,11,12,24,[36][37][38], as well as nanocarriers [15,17,19], and redox nanoprobes [15].…”

Section: Fullerenes In Affinity Biosensingmentioning

confidence: 99%

“…The current demands for reliable electrochemical biosensing require the development of sensing (bio)platforms exhibiting large surface area to allow for the high loading of capture molecules, good biocompatibility for biological activity preservation, and excellent conductivity for electron transportation [1].…”

Section: Introductionmentioning

confidence: 99%

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Fullerenes in Electrochemical Catalytic and Affinity Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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Nanotechnology is becoming increasingly important in the field of (bio)sensors. The performance and sensitivity of electrochemical biosensors can be greatly improved by the integration of nanomaterials into their construction. In this sense, carbon nanomaterials have been widely used for preparation of biosensors due to their ability to enhance electron-transfer kinetics, high surface-to-volume ratios, and biocompatibility. Fullerenes are a very promising family of carbon nanomaterials and have attracted great interest in recent years in the design of novel biosensing systems due to fullerenes' exceptional properties. These include multiple redox states, stability in many redox forms, easy functionalization and signal mediation. This paper outlines the state-of-the-art and future directions in the use and functionalization of fullerene-C 60 and its derivatives, both as electrode modifiers and advanced labels in electrochemical catalytic and affinity biosensors through selected applications.

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Recent Advances in Electrochemical Biosensors Based on Fullerene‐C60 Nano‐structured Platforms

Pilehvar

Wael

2017

Nanocarbons for Electroanalysis

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Nanotechnology is becoming increasingly important in the field of (bio)sensors. The performance and sensitivity of biosensors is greatly improved with the integration of nanomaterials into their construction. Since its first discovery, fullerene-C60 has been the object of extensive research. Its unique and favorable characteristics of easy chemical modification, conductivity, and electrochemical properties has led to its tremendous use in (bio)sensor applications. This paper provides a concise review of advances in fullerene-C60 research and its use as a nanomaterial for the development of biosensors. We examine the research work reported in the literature on the synthesis, functionalization, approaches to nanostructuring electrodes with fullerene, and outline some of the exciting applications in the field of (bio)sensing.

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An electrochemical immunosensor for sensitive detection of Escherichia coli O157:H7 using C60 based biocompatible platform and enzyme functionalized Pt nanochains tracing tag

Cited by 68 publications

References 29 publications

Biosensors for Whole-Cell Bacterial Detection

Biosensors for Whole-Cell Bacterial Detection

Fullerenes in Electrochemical Catalytic and Affinity Biosensing: A Review

Recent Advances in Electrochemical Biosensors Based on Fullerene‐C60 Nano‐structured Platforms

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