An Electron-Microscope Study Of The Trachea Of The Fowl Infected With Avian Infectious Bronchitis Virus

Uppal, P. K.; Chu, H. P.

doi:10.1099/00222615-3-4-643

Cited by 16 publications

(14 citation statements)

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“…Replication of IBV occurs in the ciliated epithelia and mucous cells within 24 hr after intratracheal or aerosol inoculation, and viral particles are confined to small vacuoles of cytoplasm [3,12,17]. Following aerosol inoculation of IBV, virus is recovered from the trachea at 24 hr and through 7 or 8 days [6].…”

Section: Discussionmentioning

confidence: 99%

Kinetics of Lymphocytic Subsets in Chicken Tracheal Lesions Infected with Infectious Bronchitis Virus.

Kotani

Wada

Tsukamoto

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. The kinetics of T-cells (CD3 positive (+), CD4+ and CD8+ cells) and B-cells (IgG+, IgM+ and IgA+ cells) in chicken trachea were studied immunohistochemically and histopathologically following an intratracheal inoculation of infectious bronchitis virus (IBV). Viral antigen was detected in the cytoplasm of tracheal epithelium from 16 hr to 6 days post-inoculation (p.i.) with a peak on 4 days p.i. A few IgG+, IgM+ and IgA+ cells were detected in the submucosa from 8 hr p.i. Thereafter IgG+ and IgM+ cells were gradually increased in number, and dramatically increased from 3 days p.i., peaked on 4 days p.i., and gradually decreased after 5 days p.i. IgA+ cells were detected in a small number than IgG+ and IgM+ cells during the all experimental period. These B cells mainly existed in the lamina propria, and some cells were recognized in the interepithelial space. After 14 days p.i., small number of IgG+ and IgM+ cells were detected in the germinal center of lymph follicles in the lamina propria. From 24 to 60 hr p.i., a few number of CD3+, CD4+ and CD8+ cells were detected at the perivascular area in the lamina propria. After 3 or 4 days p.i., each positive T-cells increased rapidly in number, and reached on the peak at 5 days p.i. CD3+, CD4+ and CD8+ cells tend to distribute diffusely, perivascular area, and surrounding area of CD4+ cells, respectively. CD4+ cells were dramatically decreased from 7 days p.i., and CD3+ and CD8+ cells were decreased from 14 days p.i. No T-cells were detected in the lymph follicles in the lamina propria.-KEY WORDS: B lymphocyte, chicken, infectious bronchitis virus, T lymphocyte, trachea.

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Section: Discussionmentioning

confidence: 99%

Kinetics of Lymphocytic Subsets in Chicken Tracheal Lesions Infected with Infectious Bronchitis Virus.

Kotani

Wada

Tsukamoto

et al. 2000

The Journal of Veterinary Medical Science

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“…Replication of IBV occurs in the ciliated epithelium and mucous cell within 24 hr after intratracheal or aerosol inoculation, and viral particles are confined to small vacuoles of cytoplasm [3,16,22]. Following aerosol inoculation of IBV, virus is recovered from the trachea at 24 hr and through 7 or 8 days [8].…”

Section: Discussionmentioning

confidence: 99%

Epithelial Cell Kinetics in the Inflammatory Process of Chicken Trachea Infected with Infectious Bronchitis Virus

Kotani

Shiraishi

Tsukamoto

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. All stages of degeneration and regeneration in chicken tracheal epithelium were studied morphologically following an intratracheal inoculation of infectious bronchitis virus (IBV). Viral antigen was detected in the cytoplasm of tracheal epithelium from 1 to 7 days post-inoculation (d.p.i.) with a peak on 3 d.p.i. At 1 d.p.i., almost all epithelial cells were involved in the degeneration. At this time, labelling index of bromodeoxyuridine (BrdU) in the basal cells showed significantly high value compared with control. At 2 and 3 d.p.i., a great number of basal cells were recognized, but the BrdU labelling index tended to decrease. At 4 and 5 d.p.i., the BrdU labelling index of basal cells significantly decreased than 1 d.p.i., and a few number of regenerated immature ciliated epithelia appeared. At 6 to 11 d.p.i., the ciliated columnar epithelia increased rapidly in number, and returned to the normal appearance except for non-ciliated patch by 13 d.p.i. These results suggested that the tracheal epithelial cells infected with IBV degenerated within 24 hours and proliferating activity of basal cells functioned immediately, and 3 to 4 days later, these basal cells were differentiated to the ciliated epithelia.-KEY WORDS: bromodeoxyuridine (BrdU), chicken, infectious bronchitis virus, regeneration, trachea.J. Vet. Med. Sci. 62(2): 129-134, 2000 (SPF) White Leghorn chickens (Line LMG) that were kindly provided by a certain breeder in Aburahi of Shiga prefecture were used in this study. Virus: The A1 strain of IBV (IBV-A1) was used in this experiment. This virus was isolated from nephritic kidneys of a 30-day-old chicken of a broiler flock in Hyogo prefecture, and it has been passaged six times in embryonated eggs and twice in chicken kidney cell culture.Experimental infection and autopsy: Thirty-six chickens were inoculated intratrachealy with 0.2 ml of viral inoculum containing 3.2 × 10 3 EID50/0.2 ml of IBV-A1. As controls, three chickens were autopsied before IBV inoculation. Clinical signs were recorded twice a day in the morning and evening. At 1-5, 7, 9, 11, 13, 17 and 21 days postinoculation (d.p.i.), three chickens were killed by pentobarbital anesthesia and the larynx and trachea were immediately removed. One hour before killing, BrdU (5-bromo-2'-deoxyuridine; Sigma Chemical) was injected subcutaneously (100 mg/kg body weight). The dorsal part of the trachea was cut vertically, and the mucous membrane was examined grossly. The tracheal specimens for light microscope (LM), for detection of viral antigen and scanning electron microscope (SEM) were collected in the respective order, 2 cm, 0.5 cm and 0.5 cm in length from the anterior part of trachea.Detection of viral antigen: The viral antigen in trachea was detected by a direct immunofluorescent method. Frozen sections, fixed with cold acetone, were incubated with fluorescein isothiocyanate (FITC)-conjugated chicken anti-IBV antibody in a moist chamber at 4°C overnight and specific fluorescence was examined under a fluorescent Since the respirator...

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“…The budding pro cess has been described in some detail in the case of avian infectious bronchitis virus, the human agent (229E [4]), and other isolates [5][6][7][8][9][10][11][12][13][14], Although most data favor budding as the assembly mechanism, some reservations have been made about the significance of this process [15,16]. During replication of the neurotropic strain JHM of mouse hepatitis virus in oligodendrocytes, within the central nervous system of rats manifesting chronic demyelinating disease, there are evident few virions and large cytoplasmic inclusions con sisting of elements resembling virus cores [17].…”

mentioning

confidence: 99%

In vivo and in vitro Models of Demyelinating Diseases

Massalski

Coulter‐Mackie

et al. 1982

Intervirology

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The developmental sequence of a neurotropic strain (JHM) of mouse hepatitis virus was examined by transmission electron microscopy and immunocytology. The nucleo-protein core of this coronavirus, which contains RNA of positive polarity and is helical in configuration, becomes incorporated into enveloped particles in the same manner as the nucleocapsids of the orthomyxo- and paramyxoviruses. However, JHM virus is assembled intracellularly by budding at surfaces of smooth membranous vacuoles. A comparison of JHMvirus replication in L2 and 17C1-1 cell lines revealed that L2 cells undergo more rapid cytopathology and cease virus production much sooner than 17C1-1 cells. In L2 cells the accumulation of core material appears to continue after the abrupt cessation of virus assembly. This is evident by the massive cytoplasmic accumulation of structures resembling nucleocapsids, which react with hybridoma antibody to the nucleocapsid antigen as demonstrated by the immunoperoxidase procedure. The current findings are consistent with our previously published demonstration, using cells of neural and other deviation, of the fundamental role of the host cell type in regulating the replication and expression of coronaviruses.

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An Electron-Microscope Study Of The Trachea Of The Fowl Infected With Avian Infectious Bronchitis Virus

Cited by 16 publications

References 4 publications

Kinetics of Lymphocytic Subsets in Chicken Tracheal Lesions Infected with Infectious Bronchitis Virus.

Kinetics of Lymphocytic Subsets in Chicken Tracheal Lesions Infected with Infectious Bronchitis Virus.

Epithelial Cell Kinetics in the Inflammatory Process of Chicken Trachea Infected with Infectious Bronchitis Virus

In vivo and in vitro Models of Demyelinating Diseases

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