An ELISA for recombinant <i>Lepidoglyphus destructor</i>, Lep d 2, and the monitoring of exposure to dust mite allergens in farming households

Parvaneh, Shahram; Johansson, E.K.; Elfman, Lena; Hage, Marianne van

doi:10.1046/j.0022-0477.2001.01255.x

Cited by 13 publications

(8 citation statements)

References 24 publications

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“…Exposure to mite allergens has been recognised worldwide as an important risk factor for development of sensitisation and allergic disease [23]. The significant source of exposure to house dust mite allergens is mattresses [24], which is in line with the current findings and our recent report where we have shown that farmers' mattresses contain high levels of mite allergens [10]. Our patient was a part-time, small-scale farmer from a relatively humid area in southeastern Sweden.…”

Section: Discussionsupporting

confidence: 90%

“…A value of >0.35 kU/l is regarded as positive. Mite allergens levels (Dermatophagoides pteronyssinus, Der p1, Dermatophagoides farinae, Der f1 and Lepidoglyphus destructor, Lep d2) were measured in a dust sample collected from the bed mattress by suctioning 1 m 2 for 2 min with a vacuum cleaner using a separate filter bag (Medeca AB, Uppsala, Sweden) and prepared and stored as described earlier [10]. Values ≥55 ng/g are regarded as positive for Der p/f1 and ≥90 ng/g for Lep d2.…”

Section: Methodsmentioning

confidence: 99%

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Death in anaphylaxis in a man with house dust mite allergy

Edston

Hage

2003

Int J Legal Med

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Up to recently the post-mortem diagnosis of anaphylaxis has been based solely on circumstantial evidence. With the development of assays for mast cell tryptase it is now possible to verify cases of suspected anaphylaxis. Here we present one such case, which initially appeared to be due to sudden death of unknown cause. A 47-year-old farmer was found dead in his bathroom around midnight. Hospital records revealed that he had previously been diagnosed with an allergy to house dust mites. He had also had infrequent episodes of airway symptoms, nausea, hypotension and diarrhoea usually after going to bed. The forensic autopsy did not give any clue to the cause of death. Serum tryptase in post-mortem blood was found to be substantially elevated in two samples (170 and >200 microg/L). Analysis of allergen-specific IgE showed high values for Dermatophagoides pteronyssinus and farinae. High mite allergen levels were found in dust obtained from the patient's mattress. The results of the immunological tests support the assumption that he died of anaphylactic shock. The circumstances and the patient's history of previous attacks after going to bed point to the fact that exposure to mite contaminated food and/or exposure to mite allergens in bed might have caused his death.

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Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Death in anaphylaxis in a man with house dust mite allergy

Edston

Hage

2003

Int J Legal Med

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“…These were analyzed using quantitative polymerase chain reaction (qPCR) techniques [7]. Allergen levels were determined using two-site sandwich ELISA for house dust mite (Der p1, Der f1), cat (Fel d1), dog (Can f1) while horse allergen (Equ cx) was measured using monoclonal antibodies [8] [9]. Due to the lack of established guidelines, data has been reported in quartiles and schools having indoor concentrations within the upper quartile were considered as having elevated levels of the particular microbiological or allergen contaminant.…”

Section: Microbiology and Allergensmentioning

confidence: 99%

The Impact of Microbiological Pollutants on School Indoor Air Quality

Fsadni¹,

Frank²,

Fsadni³

et al. 2017

GEP

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Asthma is common in children with allergens and mould influencing the development of the disease. Since children spend most of their time outside their homes within the school environment, school indoor air quality can directly influence their respiratory health. This study aims to identify microbiological contaminants in Maltese schools. The association between contaminants, respiratory health and school characteristics has been analysed. Five primary schools were selected with 9 to 11 years old students participating. Standardised health questionnaires, lung function tests, and school characterisation were performed. Dust samples were analysed for fungi, bacteria and allergens were performed. Penicillium/Aspergillus/Paecilomyces/Variotii (PenAsp) group had the highest median indoor concentration followed by Mycobacterial and Streptomyces species. There was a significant negative correlation between PenAsp and Mycobacterium spp levels in all the participating schools (r = −0.42; p = 0.03). Cat allergen in classroom dust correlated positively with the number of cat owners (r = 0.43; p = 0.041). High exposure to fungi, bacteria and allergens was significantly associated with upper and lower airway atopy. School/classroom characteristics and cleaning protocols were significantly associated with exposure to these pollutants. In conclusion, fungi, bacteria, endotoxin, cat and dog allergens have been found to have a direct influence on school indoor air quality in the Maltese Islands. A significant association was observed between these contaminants and upper and lower airway atopy. Specific school, classroom, cleaning and maintenance characteristics have been identified as having a direct impact on indoor air quality.

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“…Attention should be paid to proper cleaning practices and protection against mites. The allergens from the storage mite Lepidoglyphus destrutor, such as Lep d 1 and Lep d 2, have been purified and characterized, and a method for measuring allergens in dust samples has been developed (24)(25)(26)(27)(28). Furthermore, we need new commercial methods for measuring storage mite allergens in order to study comprehensively the possible role of mites in sensitizing laboratory animal workers.…”

Section: Discussionmentioning

confidence: 99%