An ELISA technique for the measurement of C1q in cerebrospinal fluid

Delamarche, Christian; Berger, F.; Pouplard, A; Emile, J.

doi:10.1016/0022-1759(88)90160-3

Cited by 23 publications

(18 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some published protocols also use a sandwich ELISA method, however, they use polyclonal Ab coated to the plate [3, 10]. We replicated this method as well and were unable to reproduce it.…”

Section: Resultsmentioning

confidence: 99%

“…The bottom trend line represents a standard curve obtained using a polyclonal capture Ab ( i.e. published methods [3, 10]). Using polyclonal as the capture antibody is not preferred due to the ability of polyclonal to bind many epitopes of C1q and prevent follow-up binding of primary antibodies.…”

Section: Figures and Tablesmentioning

confidence: 99%

“…The assays from [6, 9, 11, 12] use immunodiffusion which tends to have low throughput, resolution, and precision. The three ELISA assays [3, 10, 13] were not reproducible with our described components (refer to section 3.2, figure 3, and figure 4). Our assay is best suited for large numbers of sample, short assay times, and reproducibility.…”

Section: Figures and Tablesmentioning

confidence: 99%

See 2 more Smart Citations

Systemic lupus erythematosus and C1q: A quantitative ELISA for determining C1q levels in serum

Dillon

D’Souza

Kurien

et al. 2009

Biotechnology Journal

View full text Add to dashboard Cite

C1q is of interest in SLE research due to deficiencies in its activity being associated with the disease. Current published protocols for measuring C1q vary greatly in their results and ease of reproducibility. Due to this, average C1q concentrations have been reported between 56 and 276 µg/ml in non-SLE serum. We present an improved method for quantifying C1q concentrations that employs a sandwich ELISA. This method has improved precision, cost efficiency, up-scaling, reproducibility, and uses significantly lesser volumes of serum sample when compared to RID and other methods for quantifying C1q. We report an average concentration of 113±40 µg/ml for C1q in non-SLE serum. The assay designed here will be useful in the high-throughput measurement of serum C1q in SLE cases.

show abstract

“…Some published protocols also use a sandwich ELISA method, however, they use polyclonal Ab coated to the plate [3, 10]. We replicated this method as well and were unable to reproduce it.…”

Section: Resultsmentioning

confidence: 99%

Section: Figures and Tablesmentioning

confidence: 99%

Section: Figures and Tablesmentioning

confidence: 99%

See 1 more Smart Citation

Systemic lupus erythematosus and C1q: A quantitative ELISA for determining C1q levels in serum

Dillon

D’Souza

Kurien

et al. 2009

Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…Complement parameters in serum samples of the patient were measured every second month. In this case, we found continuous low levels of the classical pathway activity of complements characterized by depressed CH50, C4, C3, antigenic concentration of C1-inhibitor by methods used worldwide [9][10][11]. The level of C1q was normal.…”

Section: Case Reportmentioning

confidence: 64%

Coexistent systemic mastocytosis and essential thrombocythemia complicated with monoclonal gammopathy and hypocomplementaemia

et al. 2012

View full text Add to dashboard Cite

AbstractHematological neoplasms associated with systemic mast cell disease are most frequently of myeloid origin. There are a few reports, however, of systemic mastocytosis (SM) cases associated with lymphoid or plasma cell neoplasms as well. In this report, the authors present a case of SM (with D816V mutation in the c-KIT gene) associated with JAK2 V617F mutation negative essential thrombocythemia. The leading symptom of the 78-year-old female was recurring hydrothorax that responded only to interferon alpha therapy. During the first year of therapy, the patient developed insulin-dependent diabetes and hypothyroidism. The hematological workup also revealed IgG kappa monoclonal gammopathy that was non-progressive in the following next three years. Low levels of complements without known clinical significance accompanied the entire picture.

show abstract

“…To quantify C4‐, C3‐ and C1INH levels, radial immunodiffusion was performed using antibodies from DiaSorin (DiaSorin Inc., Stillwater, MN, USA) and a human calibator serum (Dako Denmark A/S, Glostrup, Denmark) for C3 and C4, as well as an in‐house calibrator for C1INH. Serum C1q level was determined by the ELISA method [21]. The concentration of functional C1INH was measured with a C1INH enzyme immunoassay kit (Quidel, San Diego, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Depressed activation of the lectin pathway of complement in hereditary angioedema

Varga

Laki

Kocsis

et al. 2008

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryThe possibility of simultaneous measurement of the classical pathway (CP), mannan-binding lectin (MBL)-lectin pathway (LP) and alternative pathway (AP) of complement activation by the recently developed Wielisa method allowed us to investigate the in vivo significance of the C1-inhibitor (C1INH) in three complement activation pathways. Functional activity of the CP, LP and AP were measured in the sera of 68 adult patients with hereditary angioedema (HAE) and 64 healthy controls. In addition, the level of C1q, MBL, MBL-associated serine protease-2 (MASP-2), C4-, C3-and C1INH was measured by standard laboratory methods. MBL-2 genotypes were determined by polymerase chain reaction. Besides the complement alterations (low CP and C1INH activity, low C4-, C1INH concentrations), which characterize HAE, the level of MASP-2 was also lower (P = 0·0001) in patients compared with controls. Depressed LP activity was found in patients compared with controls (P = 0·0008) in homozygous carriers of the normal MBL genotype (A/A), but not in carriers of variant genotypes (A/O, O/O). Activity of CP correlated with LP in patients (Spearman's r = 0·64; P < 0·0001), but no significant correlation was found in the control group and no correlation with AP was observed. In contrast, the activity of CP and AP correlated (Spearman's r = 0·47; P < 0·0001) in healthy controls, but there was no significant correlation in the HAE patients. We conclude that the activation of LP might also occur in subjects with C1INH deficiency, which is reflected by the low MASP-2 and C4 levels.

show abstract

An ELISA technique for the measurement of C1q in cerebrospinal fluid

Cited by 23 publications

References 15 publications

Systemic lupus erythematosus and C1q: A quantitative ELISA for determining C1q levels in serum

Systemic lupus erythematosus and C1q: A quantitative ELISA for determining C1q levels in serum

Coexistent systemic mastocytosis and essential thrombocythemia complicated with monoclonal gammopathy and hypocomplementaemia

Depressed activation of the lectin pathway of complement in hereditary angioedema

Contact Info

Product

Resources

About