An endogastric capsule for measuring tumor markers ingastric juice: an evaluation of the safety and efficacy of a new diagnostic tool

Muretto, Pietro; Graziano, Francesco; Staccioli, Maria Pia; Barbanti, I; Bartolucci, A. A.; Paolini, G.; Giordano, Daniela; Testa, E.; Gaetano, Andrea De

doi:10.1093/annonc/mdg027

Cited by 23 publications

(23 citation statements)

References 28 publications

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“…As previously demonstrated by the pertinent literature, too many variables seem to impact on the intragastric concentrations of tumor markers thereby affecting the results of a systematic analysis: above all, the inconsistent and uncontrollable direction of cancer cell dissemination (apical vs. basolateral secretion, i.e. the endoluminal vs. the classical route of metastasis), the presence of endoluminal obstruction determined by the tumor itself, the coexistence of other inflammatory, infectious, or precancerous lesions involving gastric mucosa, as well as endoscopic maneuvers (taking biopsies with diagnostic intent) [15,20,21,22,23,24]. …”

Section: Discussionmentioning

confidence: 99%

“…The first three, which are currently routinely investigated in the serum of GC patients for diagnosis and surveillance, have already been evaluated in GL of GC patients in previous studies [9,10,11,12,13,14,15]. Of interest, only one previous study dealt with the detection of CA 50 in biological fluids of GC patients other than serum, and that was not GL [16].…”

Section: Methodsmentioning

confidence: 99%

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Analyzing Gastric Lavage of Gastric Cancer Patients: A Prospective Observational Study on Cytopathology and Determination of Intragastric CEA, CA 19.9, CA 72.4, and CA 50

Virgilio¹,

Giarnieri²,

Montagnini³

et al. 2016

Acta Cytologica

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Objectives: To investigate gastric lavage (GL) cytopathology and immunometric analysis as novel clinicopathologic and prognostic parameters for gastric cancer (GC). Study Design: In 38 patients with gastric adenocarcinoma, we performed a cytopathologic analysis and an immunometric assay of GL using four tumor markers (CEA, CA 19.9, CA 72.4, and CA 50). The intragastric tumor marker levels were compared with a control group consisting of 41 non-GC patients to determine a statistically significant cutoff value. Results: GL cytopathology demonstrated the presence of cancer cells in 13 (34.2%) of the 38 GC patients: such a finding correlated to the parameters pT and pN with a statistically significant validity (p < 0.0267 and p < 0.0306, respectively). Measurement of intragastric CA 19.9 and CA 50 attained a statistically significant cutoff value (p < 0.002 and p < 0.0096, respectively), which was invalidated by the low sensitivity of the ROC curve analysis. Conclusions: In contrast to determination of its tumor markers, GL cytopathology correlated well with pT and pN staging parameters. Should this and other features be corroborated by future studies, the GL cytology test could be routinely used to detect aggressive types of GC even at early stages and result in important progress in the knowledge, staging, prediction, as well as management and follow-up of this inauspicious type of cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Analyzing Gastric Lavage of Gastric Cancer Patients: A Prospective Observational Study on Cytopathology and Determination of Intragastric CEA, CA 19.9, CA 72.4, and CA 50

Virgilio¹,

Giarnieri²,

Montagnini³

et al. 2016

Acta Cytologica

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“…Recently, a small gelatin capsule containing a pierced plastic cover surrounding a piece of absorbent paper was developed to obtain gastric juice (5). Additionally, a noninvasive string test has also been reported useful to obtain gastric juice samples for the diagnosis of H. pylori infection (6).…”

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confidence: 99%

α1-Antitrypsin Precursor in Gastric Juice Is a Novel Biomarker for Gastric Cancer and Ulcer

Hsu

Chen

Hsieh

et al. 2007

Clinical Cancer Research

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Purpose: To search for novel disease-specific markers in gastric juice by investigating the protein concentrations and components in gastric juice from patients with various gastroduodenal diseases. Experimental Design: Protein concentrations and pH values in fasting gastric juice were examined in 120 healthy subjects and 39 gastric ulcer, 38 duodenal ulcer, and 31 gastric cancer patients. The protein components in gastric juice were studied by two-dimensional PAGE and mass spectrometric analysis. Results: Protein concentrations in gastric juice of patients with gastric ulcers and gastric cancer were significantly higher than those in healthy subjects (1.06 and 2.61 mg/mL versus 0.48 mg/mL; P = 0.001and P < 0.001, respectively), and duodenal ulcer patients had lower gastric juice protein concentrations compared with healthy subjects (0.26 versus 0.48 mg/mL; P < 0.05). Gastric hypoacidity and advanced age were independent factors affecting the protein concentrations in gastric juice with odds ratios of 32.9 (95% confidence interval, 11.8-90.9) and 3.2 (95% confidence interval, 1.3-8.3), respectively. Each electrophoresis images of gastric juice could be classified into one of three patterns: basic band, specific band, or nonspecific band. The frequencies of specific band pattern in healthy subjects, gastric ulcer, duodenal ulcer, and gastric cancer patients were 6%, 42%, 6%, and 93%, respectively. Proteomic analysis revealed that a1-antitrypsin precursor was the principal peptide in the specific band. Conclusions: a1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer. A noninvasive method to obtain gastric juice followed by proteomic analysis may serve as a new tool to screen for gastric malignancies.

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“…Various noninvasive methods have recently been developed for obtaining gastric juices (13)(14)(15). For example, a noninvasive string test has been reported to get gastric juice samples for the diagnosis of Helicobacter pylori infection (13,14).…”

Section: Introductionmentioning

confidence: 99%

“…For example, a noninvasive string test has been reported to get gastric juice samples for the diagnosis of Helicobacter pylori infection (13,14). Additionally, a capsule method containing a pierced plastic cover and a piece of absorbent paper has also been developed to get gastric juice (15). We therefore designed this study to investigate the differences of α1-antitrypsin concentrations in gastric juice between patients with gastric cancer and benign gastroduodenal diseases, and to develop a novel noninvasive gastric juice α1-antitrypsin test for cancer screening using a string test to obtain gastric juice.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Gastric Malignancy Using Gastric Juice α1-antitrypsin

Hsu

Chen

Hsiao

et al. 2010

Cancer Epidemiology, Biomarkers &Amp; Prevention

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No accurate, inexpensive, and noninvasive test for gastric cancer screening is currently available. Our recent study identified α1-antitrypsin as a potential biomarker of gastric cancer in gastric juice. The aim of this study was to develop a novel noninvasive modality for detecting gastric cancer by measurement of α1-antitrypsin concentration in gastric juice. The work consisted of two parts: (a) investigating the differences in gastric juice α1-antitrypsin concentrations between gastric cancer patients and controls, and (b) screening gastric cancer using string test to obtain gastric juice followed by immunoassay for α1-antitrypsin concentration. The data showed that gastric juice α1-antitrypsin concentration was markedly higher in gastric cancer patients than in healthy subjects, gastric ulcer patients, and duodenal ulcer patients (all P < 0.001). The area under the receiver operating characteristic curve for identifying gastric cancer cases was 0.96 (95% confidence interval, 0.93-0.99; P < 0.001). The sensitivity and specificity of gastric juice α1-antitrypsin concentration were 96% and 92%, respectively. Gastric juice α1-antitrypsin assay through string test was validated in 93 consecutive patients for gastric cancer screening. The sensitivity and specificity of gastric juice α1-antitrypsin string test at 85% accuracy were 74% and 88%, respectively. The area under the receiver operating characteristic curve for identifying gastric cancer was 0.84. In conclusion, gastric juice α1-antitrypsin concentration in gastric cancer patients markedly exceeds those in healthy subjects and patients with benign gastrointestinal diseases. A noninvasive α1-antitrypsin string test may serve as a new screening tool for identifying gastric cancer patients. Cancer Epidemiol Biomarkers Prev; 19(2); 405-11. ©2010 AACR.

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An endogastric capsule for measuring tumor markers ingastric juice: an evaluation of the safety and efficacy of a new diagnostic tool

Cited by 23 publications

References 28 publications

Analyzing Gastric Lavage of Gastric Cancer Patients: A Prospective Observational Study on Cytopathology and Determination of Intragastric CEA, CA 19.9, CA 72.4, and CA 50

Analyzing Gastric Lavage of Gastric Cancer Patients: A Prospective Observational Study on Cytopathology and Determination of Intragastric CEA, CA 19.9, CA 72.4, and CA 50

α1-Antitrypsin Precursor in Gastric Juice Is a Novel Biomarker for Gastric Cancer and Ulcer

Diagnosis of Gastric Malignancy Using Gastric Juice α1-antitrypsin

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