An endophytic fungus efficiently producing paclitaxel isolated from Taxus wallichiana var. mairei

Zaiyou, Jian; Li, Meng; Xi-qiao, HU

doi:10.1097/md.0000000000007406

Cited by 56 publications

(21 citation statements)

References 15 publications

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“…Endophytes of 18 fungal taxonomic groups, for example, Aspergillus fumigatus , Colletotrichum gloeosporioides , and Fusarium sp. , were found to produce paclitaxel . These fungi were also found, with different abundance, in Taxus rhizosphere of this study.…”

Section: Discussionsupporting

confidence: 53%

The first Taxus rhizosphere microbiome revealed by shotgun metagenomic sequencing

2018

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In the present study, the shotgun high throughput metagenomic sequencing was implemented to globally capture the features of Taxus rhizosphere microbiome. Total reads could be assigned to 6925 species belonging to 113 bacteria phyla and 301 species of nine fungi phyla. For archaea and virus, 263 and 134 species were for the first time identified, respectively. More than 720,000 Unigenes were identified by clean reads assembly. The top five assigned phyla were Actinobacteria (363,941 Unigenes), Proteobacteria (182,053), Acidobacteria (44,527), Ascomycota (fungi; 18,267), and Chloroflexi (15,539). KEGG analysis predicted numerous functional genes; 7101 Unigenes belong to "Xenobiotics biodegradation and metabolism." A total of 12,040 Unigenes involved in defense mechanisms (e.g., xenobiotic metabolism) were annotated by eggNOG. Talaromyces addition could influence not only the diversity and structure of microbial communities of Taxus rhizosphere, but also the relative abundance of functional genes, including metabolic genes, antibiotic resistant genes, and genes involved in pathogen-host interaction, bacterial virulence, and bacterial secretion system. The structure and function of rhizosphere microbiome could be sensitive to non-native microbe addition, which could impact on the pollutant degradation. This study, complementary to the amplicon sequencing, more objectively reflects the native microbiome of Taxus rhizosphere and its response to environmental pressure, and lays a foundation for potential combination of phytoremediation and bioaugmentation.

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Section: Discussionsupporting

confidence: 53%

The first Taxus rhizosphere microbiome revealed by shotgun metagenomic sequencing

2018

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“…By HPLC analyzes, they obtained 76 μg L -1 of the compound. Also Zaiyou et al (2015) found in the extract of the endophyte Fusarium sp. The compound paclitaxel at the concentration of 0.0153 mg L -1 .…”

Section: Discussionmentioning

confidence: 99%

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Ferreira¹,

Santos²,

Soares³

et al. 2017

J. Med. Plants Res.

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Lippia sidoidesCham. (Verbenaceae) is a species native to the Brazilian northeast, widely used in popular medicine. Its leaves were used for the isolation of endophytic fungi and extraction of metabolites. Among them, three were selected according to fungitoxicity tests against the maize phytopathogenic fungus, Curvularia lunata (Wakker). However, the objective of this study was to identify the role of L. sidoides extracts associated with their endophytic fungi, necessary to reduce excess of fungicides applied on the maize crop. Metabolites were evaluated for antioxidant activity by 2.2-diphenyl-1-picrylhydrazyl (DPPH), phenols, total flavonoids and one of it endophytic fungus were evaluated for synergism (Verticilium sp. and plant extracts). The endophytic fungi and plant extracts evaluated for phenolic content ranged from 0.29 ± 0.05 to 96.94 ± 11.86 mgEAG/g, the content of flavonoids from 14.31 ± 1.56 to 192.33 ± 4.58 mgER/g, and antioxidant activity could only be observed for the plant extract with EC 50 81 ± 0.3%. The secondary metabolites identified by HPLC in the plant extract were catechin, quercetin, gallic acid and naringin. Naringenin, catechin, epigallocatechin gallate and quercetin were identified in the extract of the fungi viz. Verticillium sp. and Fusarium sp. Synergistic analysis between a 1:1 proportion of plant and fungal extracts has shown more efficient (79.0%) inhibition of C. lunata. Thus, alternative control of phytopathogenic fungi can be accomplished using plant extracts associated with their endophytic fungi, reducing the excess of fungicides applied on the maize crop.

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“…Paclitaxel production from endophytes is reported often in submerged culture (Wang et al, 2001 ; Zaiyou et al, 2017 ). Paclitaxel in the fungal extract can be detected by various analytical (Thin layer chromatography, High-performance liquid chromatography), spectroscopic (Matrix-assisted laser desorption/ionization—Time of flight [MALDI-TOF], Nuclear magnetic resonance [NMR], Fast atom bombardment [FAB], Electron spray ionization [ESI]), and immunological techniques (using monoclonal antibodies specific for paclitaxel) (Flores-Bustamante et al, 2010 ).…”

Section: Clinically Used Anticancer Drugs and Their Precursors From Ementioning

confidence: 99%

Endophytic Fungi—Alternative Sources of Cytotoxic Compounds: A Review

et al. 2018

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Cancer is a major cause of death worldwide, with an increasing number of cases being reported annually. The elevated rate of mortality necessitates a global challenge to explore newer sources of anticancer drugs. Recent advancements in cancer treatment involve the discovery and development of new and improved chemotherapeutics derived from natural or synthetic sources. Natural sources offer the potential of finding new structural classes with unique bioactivities for cancer therapy. Endophytic fungi represent a rich source of bioactive metabolites that can be manipulated to produce desirable novel analogs for chemotherapy. This review offers a current and integrative account of clinically used anticancer drugs such as taxol, podophyllotoxin, camptothecin, and vinca alkaloids in terms of their mechanism of action, isolation from endophytic fungi and their characterization, yield obtained, and fungal strain improvement strategies. It also covers recent literature on endophytic fungal metabolites from terrestrial, mangrove, and marine sources as potential anticancer agents and emphasizes the findings for cytotoxic bioactive compounds tested against specific cancer cell lines.

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An endophytic fungus efficiently producing paclitaxel isolated from Taxus wallichiana var. mairei

Cited by 56 publications

References 15 publications

The first Taxus rhizosphere microbiome revealed by shotgun metagenomic sequencing

The first Taxus rhizosphere microbiome revealed by shotgun metagenomic sequencing

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Endophytic Fungi—Alternative Sources of Cytotoxic Compounds: A Review

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