An endoplasmic reticulum magnesium transporter is essential for pollen development in Arabidopsis

Li, Jian; Huang, Yuan; Tan, H.S.; Yang, Xiao; Tian, Lianfu; Luan, Sheng; Chen, Liangbi; Li, Dong-Ping

doi:10.1016/j.plantsci.2014.12.008

Cited by 54 publications

(35 citation statements)

References 44 publications

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“…The Pro NCED 3 -GUS transgenic plants (T 1 ) were used to histochemical staining analysis. Histochemical staining was conducted as previously described (Li et al, 2015 ). The tissues of Pro NCED 3 -GUS transgenic plants (T 1 ) were incubated in the GUS staining solution (50 mM sodium phosphate at pH 7.0, 10 mM EDTA, 0.5 mM potassium ferricyanide, 0.5 mM potassium ferrocyanide, 10% methanol, 0.1% Triton X-100, and 1 mg mL −1 of X-Gluc (Sangon, Shanghai, China) dissolved in N,N-dimethyl formamide) at 37°C for 10 h, the samples were incubated in 75% (v/v) ethanol to clear the chlorophyll.…”

Section: Methodsmentioning

confidence: 99%

9-cis-Epoxycarotenoid Dioxygenase 3 Regulates Plant Growth and Enhances Multi-Abiotic Stress Tolerance in Rice

et al. 2018

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Although abscisic acid (ABA) is an important hormone that regulates seed dormancy, stomatal closure, plant development, as well as responses to environmental stimuli, the physiological mechanisms of ABA response to multiple stress in rice remain poorly understood. In the ABA biosynthetic pathway, 9-cis-epoxycarotenoid dioxygenase (NCED) is the key rate-limiting enzyme. Here, we report important functions of OsNCED3 in multi-abiotic stress tolerance in rice. The OsNCED3 is constitutively expressed in various tissues under normal condition, Its expression is highly induced by NaCl, PEG, and H2O2 stress, suggesting the roles for OsNCED3 in response to the multi-abiotic stress tolerance in rice. Compared with wild-type plants, nced3 mutants had earlier seed germination, longer post-germination seedling growth, increased sensitivity to water stress and H2O2 stress and increased stomata aperture under water stress and delayed leaf senescence. Further analysis found that nced3 mutants contained lower ABA content compared with wild-type plants, overexpression of OsNCED3 in transgenic plants could enhance water stress tolerance, promote leaf senescence and increase ABA content. We conclude that OsNCED3 mediates seed dormancy, plant growth, abiotic stress tolerance, and leaf senescence by regulating ABA biosynthesis in rice; and may provide a new strategy for improving the quality of crop.

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Section: Methodsmentioning

confidence: 99%

9-cis-Epoxycarotenoid Dioxygenase 3 Regulates Plant Growth and Enhances Multi-Abiotic Stress Tolerance in Rice

et al. 2018

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“…Other pollen wall development-associated transporters include sugar transporter RPG1 for primexine deposition [20], magnesium transporters (AtMGT4, AtMGT[ 1 0 _ T D $ D I F F ] 5, and AtMGT9) for pollen development [66], and REPRESSOR OF CYTOKININ DEFICIENCY 1 (ROCK1), which transfers UDP-GlcNAc and UDPGalNAc for sporopollenin and primexine formation [67]. These findings suggest that the functions of proteins responsible for pollen wall precursor allocation are diversified in plants, and need to be characterized further at the biochemical and anatomical levels.…”

Section: Transport Of Precursors Across Anther Tissuesmentioning

confidence: 99%

Genetic and Biochemical Mechanisms of Pollen Wall Development

Shi

Cui

et al. 2015

Trends in Plant Science

346

410

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“…MGT2 and MGT3 are associated with vacuolar membrane and possibly involved in Mg 2+ homeostasis in leaf mesophyll cells ( Conn et al, 2011 ). Quite a few MGTs including MGT4, MGT5, and MGT9 are highly expressed in pollen and anther cells, and are required for plant reproduction, suggesting that active Mg 2+ transport is critical for pollen development ( Li et al, 2008 , 2015 ; Chen et al, 2009 ; Xu et al, 2015 ). MGT10 is localized in the chloroplast envelope, and is strongly expressed in the rosette and cauline leaves, indicating its possible function in Mg 2+ translocation into chloroplasts ( Drummond et al, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Magnesium Transporter MGT6 Plays an Essential Role in Maintaining Magnesium Homeostasis and Regulating High Magnesium Tolerance in Arabidopsis

et al. 2018

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Magnesium (Mg) is one of the essential nutrients for all living organisms. Plants acquire Mg from the environment and distribute within their bodies in the ionic form via Mg2+-permeable transporters. In Arabidopsis, the plasma membrane-localized magnesium transporter MGT6 mediates Mg2+ uptake under Mg-limited conditions, and therefore is important for the plant adaptation to low-Mg environment. In this study, we further assessed the physiological function of MGT6 using a knockout T-DNA insertional mutant allele. We found that MGT6 was required for normal plant growth during various developmental stages when the environmental Mg2+ was low. Interestingly, in addition to the hypersensitivity to Mg2+ limitation, mgt6 mutants displayed dramatic growth defects when external Mg2+ was in excess. Compared with wild-type plants, mgt6 mutants generally contained less Mg2+ under both low and high external Mg2+ conditions. Reciprocal grafting experiments further underpinned a role of MGT6 in a shoot-based mechanism for detoxifying excessive Mg2+ in the environment. Moreover, we found that mgt6 mgt7 double mutant showed more severe phenotypes compared with single mutants under both low- and high-Mg2+ stress conditions, suggesting that these two MGT-type transporters play an additive role in controlling plant Mg2+ homeostasis under a wide range of external Mg2+ concentrations.

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An endoplasmic reticulum magnesium transporter is essential for pollen development in Arabidopsis

Cited by 54 publications

References 44 publications

9-cis-Epoxycarotenoid Dioxygenase 3 Regulates Plant Growth and Enhances Multi-Abiotic Stress Tolerance in Rice

9-cis-Epoxycarotenoid Dioxygenase 3 Regulates Plant Growth and Enhances Multi-Abiotic Stress Tolerance in Rice

Genetic and Biochemical Mechanisms of Pollen Wall Development

Magnesium Transporter MGT6 Plays an Essential Role in Maintaining Magnesium Homeostasis and Regulating High Magnesium Tolerance in Arabidopsis

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