An Engineered Protein-Based Building Block (Albumin Methacryloyl) for Fabrication of a 3D In Vitro Cryogel Model

Niu, Xianjun; Lin, Mian; Lee, Bae Hoon

doi:10.3390/gels8070404

Cited by 7 publications

(8 citation statements)

References 76 publications

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“…Therefore, CCK‐8 results and CLSM images demonstrated that HSAMA 4 hydrogels displayed good biocompatibility regardless of different HSAMA 4 concentrations, which is desired for tissue engineering applications. [ 106,107 ]…”

Section: Resultsmentioning

confidence: 99%

Human Albumin‐Based Hydrogels for Their Potential Xeno‐Free Microneedle Applications

Rong

Mehwish

Niu

et al. 2023

Macromolecular Bioscience

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Nowadays, hydrogels-based microneedles (MNs) have attracted a great interest owing to their outstanding qualities for biomedical applications. For the fabrication of hydrogels-based microneedles as tissue engineering scaffolds and drug delivery carriers, various biomaterials have been tested. They are required to feature tunable physiochemical properties, biodegradability, biocompatibility, nonimmunogenicity, high drug loading capacity, and sustained drug release. Among biomaterials, human proteins are the most ideal biomaterials for fabrication of hydrogels-based MNs; however, they are mechanically weak and poorly processible. To the best of the knowledge, there are no reports of xeno-free human protein-based MNs so far. Here, human albumin-based hydrogels and microneedles for tissue engineering and drug delivery by using relatively new processible human serum albumin methacryloyl (HSAMA) are engineered. The resultant HSAMA hydrogels display tunable mechanical properties, biodegradability, and good biocompatibility. Moreover, the xeno-free HSAMA microneedles display a sustained drug release profile and significant mechanical strength to penetrate the model skin. In vitro, they also show good biocompatibility and anticancer efficacy. Sustainable processible human albumin-based biomaterials may be employed as a xeno-free platform in vivo for tissue engineering and drug delivery in clinical trials in the future.

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Section: Resultsmentioning

confidence: 99%

Human Albumin‐Based Hydrogels for Their Potential Xeno‐Free Microneedle Applications

Rong

Mehwish

Niu

et al. 2023

Macromolecular Bioscience

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“…Bovine serum albumin methacryloyl (BSAMA) cryogels were prepared according to a previous report [ 8 ]. Briefly, BSAMA powder was dissolved in PBS to form a clear BSAMA solution of 3%, 5%, and 10%.…”

Section: Methodsmentioning

confidence: 99%

“…Cryogels, as an example, differ from conventional hydrogels in that they display a 3D network of massive interconnected pores, highly crosslinked polymer walls, and excellent mechanical capabilities [ 6 ]. Cryogels can also be made for suturable intraoral grafts [ 7 ], and in vitro models that may replicate the microenvironments of the liver [ 8 ]. More importantly, cryogels support a somewhat strong resilience to autoclave (moist heat) sterilization, which is used as one of the terminal sterilization methods [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Autoclavable Albumin-Based Cryogels with Uncompromising Properties

Duan,

Mehwish,

et al. 2023

Gels

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The development of autoclavable hydrogels has been driven by the need for materials that can withstand the rigors of sterilization without compromising their properties or functionality. Many conventional hydrogels cannot withstand autoclave treatment owing to the breakdown of their composition or structure under the high-temperature and high-pressure environment of autoclaving. Here, the effect of autoclaving on the physical, mechanical, and biological properties of bovine serum albumin methacryloyl (BSAMA) cryogels at three protein concentrations (3, 5, and 10%) was extensively studied. We found that BSAMA cryogels at three concentrations remained little changed after autoclaving in terms of gross shape, pore structure, and protein secondary structure. Young’s modulus of autoclaved BSAMA cryogels (BSAMAA) at low concentrations (3 and 5%) was similar to that of BSAMA cryogels, whereas 10% BSAMAA exhibited a higher Young’s modulus value, compared with 10% BSAMA. Interestingly, BSAMAA cryogels prolonged degradation. Importantly, cell viability, drug release, and hemolytic behaviors were found to be similar among the pre- and post-autoclaved cryogels. Above all, autoclaving proved to be more effective in sterilizing BSAMA cryogels from bacteria contamination than UV and ethanol treatments. Thus, autoclavable BSAMA cryogels with uncompromising properties would be useful for biomedical applications.

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“…9,102 Since specific ECM compounds can be very expensive, there is an option to produce scaffolds from more cost-efficient polymers and afterwards applying a bio-active coating to enhance cell adhesion and spreading. 103 Often used coating compounds in this regard include laminin, Fibronectin and collagen. [104][105][106] Alternative strategies described in literature include the application of decellularized native ECM (dECM) compounds used as biomaterial ink for scaffold fabrication/ printing or the decellularization and subsequent recellularization of natural liver tissue matrix.…”

Section: Reviewmentioning

confidence: 99%