An enhanced analytical procedure to discover table grape DNA adulteration in industrial musts

Rienzo, Valentina di; Miazzi, Monica Marilena; Fanelli, Valentina; Savino, V.; Pollastro, Stefania; Colucci, Francesco; Miccolupo, Angela; Blanco, Antonio; Pasqualone, Antonella; Montemurro, Cinzia

doi:10.1016/j.foodcont.2015.07.015

Cited by 36 publications

(23 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years there has been an increasing number of reports of the use of HRM for species identification, genotyping, and food identification . The HRM method was also used to quantify the presence of adulterants such as different species or botanical varieties . Montemurro et al .…”

Section: Resultsmentioning

confidence: 99%

“…33,40 The HRM method was also used to quantify the presence of adulterants such as different species or botanical varieties. 41,42 Montemurro et al 43 used HRM analysis to distinguish Terra di Bario olive oil from non-Terradi Bari olive oil using different mixtures. Recently, the bar-HRM method, which combines plant barcoding with HRM analysis, was developed to identify the seven native Chinese Hippophae species and to authenticate commercial sea buckthorn products.…”

Section: Hrm Analysis Of Laboratory Experimental Mixtures and Walnut mentioning

confidence: 99%

See 1 more Smart Citation

DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication

et al. 2020

View full text Add to dashboard Cite

Background Walnut (Juglans regia L.) is one of the most widely cultivated nuts. Walnut milk beverage is very popular in China due to its nutritional value. However, adulterated walnut milk ingredients have been detected in the Chinese market. Peanut and soybean are sold at much lower prices than walnut and are reported to be commonly used for adulteration in the industrial chain of walnut milk production. The purpose of this study is therefore to develop an accurate and efficient method for detecting the authenticity of the raw materials used in walnut milk beverage. Results DNA barcoding and high‐resolution melting (HRM) analyses were used to identify common adulterated raw ingredients such as peanut and soybean in commercial walnut milk beverage samples. The chloroplast psbA‐trnH gene was used for sequencing, and HRM analysis was performed. We also prepared experimental mixtures, in the laboratory, with different quantities of walnut, peanut, and soybean. High‐resolution melting analysis of the experimental mixtures clearly distinguished all of them. The results revealed that most of the walnut milk beverage samples fell in the same cluster of walnut species. Several samples fell in the peanut cluster, confirming that they were adulterated products. Conclusion The results revealed that HRM analysis based on the psbA‐trnH barcode sequence can be used to identify raw ingredients in walnut milk beverages. © 2020 Society of Chemical Industry

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Hrm Analysis Of Laboratory Experimental Mixtures and Walnut mentioning

confidence: 99%

DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication

et al. 2020

View full text Add to dashboard Cite

show abstract

“…More recently, microsatellites have been analysed by taking advantage of HRM, which allowed to detect admixtures of single‐cultivar oils with alien olive cultivars, at levels as low as 10%, by comparing the temperature‐shifted difference curves . This result evidenced the possibility to use the HRM technique not only for qualitative application but also for quantitative detection of the adulteration level, as already done in other food chains …”

Section: Application Of Dna Markers To Extra Virgin Olive Oil and Tabmentioning

confidence: 90%

“…99 This result evidenced the possibility to use the HRM technique not only for qualitative application but also for quantitative detection of the adulteration level, as already done in other food chains. 199,200 Real-time quantitative PCR (qRT-PCR) is the technique commonly used for detection and quantification of nucleic acids. This technique has been used in selecting primers for olive oil authentication, so as to help in discarding primers with low PCR efficiency, 182 and could represent an effective tool for quantitative analysis of cultivar contribution in blended olive oils, as already done in vegetable oils for species traceability and GMO detection.…”

Section: Application Of Dna Markers To Extra Virgin Olive Oil and Tabmentioning

confidence: 99%

Evolution and perspectives of cultivar identification and traceability from tree to oil and table olives by means of DNA markers

et al. 2016

Self Cite

View full text Add to dashboard Cite

In recent years, an increasing number of typicality marks has been awarded to high-quality olive oils produced from local cultivars. In this case, quality control requires effective varietal checks of the starting materials. Moreover, accurate cultivar identification is essential in vegetative-propagated plants distributed by nurseries and is a pre-requisite to register new cultivars. Food genomics provides many tools for cultivar identification and traceability from tree to oil and table olives. The results of the application of different classes of DNA markers to olive with the purpose of checking cultivar identity and variability of plant material are extensively discussed in this review, with special regard to repeatability issues and polymorphism degree. The characterization of olive germplasm from all countries of the Mediterranean basin and from less studied geographical areas is described and innovative high-throughput molecular tools to manage reference collections are reviewed. Then the transferability of DNA markers to processed products - virgin olive oils and table olives - is overviewed to point out strengths and weaknesses, with special regard to (i) the influence of processing steps and storage time on the quantity and quality of residual DNA, (ii) recent advances to overcome the bottleneck of DNA extraction from processed products, (iii) factors affecting whole comparability of DNA profiles between fresh plant materials and end-products, (iv) drawbacks in the analysis of multi-cultivar versus single-cultivar end-products and (v) the potential of quantitative polymerase chain reaction (PCR)-based techniques. © 2016 Society of Chemical Industry.

show abstract

“…To be effective, these techniques need to take into account numerous factors that might influence the product, such as the climate and soil type, or the use of fertilizers and pesticides, not to mention the wine-making practices. As an example, techniques based on DNA analysis, which is independent from environmental influences, can be considered for traceability, especially for Protected Designation of Origin (PDO) products [3,4].…”

Section: Introductionmentioning

confidence: 99%

Wine Traceability with Rare Earth Elements

et al. 2018

View full text Add to dashboard Cite

Abstract:The traceability of foodstuffs is now a relevant aspect of the food market. Scientific research has been devoted to addressing this issue by developing analytical protocols in order to find the link between soil and food items. In this view, chemical parameters that can act as soil markers are being sought. In this work, the role of rare earth elements (REEs) as geochemical markers in the traceability of red wine is discussed. The REE distribution in samples from each step of the wine making process of Primitivo wine (produced in Southern Italy) was determined using the highly sensitive inductively coupled plasma-mass spectrometry (ICP-MS) technique. Samples analyzed include grapes, must, and wine samples after every step in the vinification process. The resulting data were compared to the REE distribution in the soil, revealing that the soil fingerprint is maintained in the intermediate products up to and including grape must. Fractionation occurs thereafter as a consequence of further external interventions, which tends to modify the REE profile.

show abstract

An enhanced analytical procedure to discover table grape DNA adulteration in industrial musts

Cited by 36 publications

References 44 publications

DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication

DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication

Evolution and perspectives of cultivar identification and traceability from tree to oil and table olives by means of DNA markers

Wine Traceability with Rare Earth Elements

Contact Info

Product

Resources

About